Q9L5C8 (Q9L5C8_ECOLX) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Beta-lactamase EMBL CAJ13580.1

Submitted name:
Beta-lactamase CTX-M EMBL ADJ19345.1
Submitted name:
Beta-lactamase CTX-M-9a EMBL AAF72529.1
Submitted name:
Betalactamase CTX-M-9 EMBL AAF05311.2
Submitted name:
CTX-M-9 beta-lactamase EMBL CAC95176.1
Submitted name:
CTX-M-9 extended-spectrum beta-lactamase EMBL ACM04461.1

Gene names

Name:	blaCTX-M-9a EMBL AAF72529.1
Synonyms:	blaCTX-M-9 EMBL AAF05311.2, blaCTX-M-9b EMBL ACM04461.1

Encoded on

Plasmid pOZ173 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.14 EMBL AAF72529.1
Plasmid pMSP071 Ref.1 Ref.10 EMBL AAF05311.2

Organism

Escherichia coli EMBL AAF72529.1

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid. SAAS SAAS012338

Ontologies

Keywords
Biological process	Antibiotic resistance SAAS SAAS012338
Molecular function	Hydrolase SAAS SAAS023650
Technical term	3D-structure PDB 1YLJ PDB 1YM1 PDB 1YMS PDB 3G2Z PDB 3HUO PDB 3HLW PDB 3HRE PDB 1YMX PDB 3G2Y PDB 3HVF PDB 3G34 PDB 3G35 PDB 2P74 PDB 3G30 PDB 3G31 PDB 3G32 Plasmid EMBL AAF72529.1
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Binding site	73	1	Cefoxitin (covalent) PDB 1YMX
	Binding site	103	1	Sucrose; via carbonyl oxygen PDB 1YLJ
	Binding site	139	1	Sucrose; via carbonyl oxygen PDB 1YLJ
	Binding site	168	1	Sucrose PDB 1YLJ

Sequences

	Sequence		Length	Mass (Da)
	Q9L5C8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C8CB0A7739B9469E	FASTA	291	30,951
	10 20 30 40 50 60 MVTKRVQRMM FAAAACIPLL LGSAPLYAQT SAVQQKLAAL EKSSGGRLGV ALIDTADNTQ 70 80 90 100 110 120 VLYRGDERFP MCSTSKVMAA AAVLKQSETQ KQLLNQPVEI KPADLVNYNP IAEKHVNGTM 130 140 150 160 170 180 TLAELSAAAL QYSDNTAMNK LIAQLGGPGG VTAFARAIGD ETFRLDRTEP TLNTAIPGDP 190 200 210 220 230 240 RDTTTPRAMA QTLRQLTLGH ALGETQRAQL VTWLKGNTTG AASIRAGLPT SWTAGDKTGS 250 260 270 280 290 GDYGTTNDIA VIWPQGRAPL VLVTYFTQPQ QNAESRRDVL ASAARIIAEG L

References

[1]	"Cloning and sequence of the gene encoding a novel cefotaxime-hydrolyzing beta-lactamase (CTX-M-9) from Escherichia coli in Spain." Sabate M., Tarrago R., Navarro F., Miro E., Verges C., Barbe J., Prats G. Antimicrob. Agents Chemother. 44:1970-1973(2000) [PubMed: 10858363] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 785-D EMBL AAF05311.2.
[2]	"Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals." Saladin M., Bao Cao V.T., Lambert T., Donay J.L., Herrmann J.L., Ould Hocine Z., Verdet C., Delisle F., Philippon A., Arlet G. FEMS Microbiol. Lett. 209:161-168(2002) [PubMed: 12007800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: RAJ EMBL CAC95176.1.
[3]	"Dominance of blaCTX-M within an Australian extended-spectrum beta-lactamase gene pool." Zong Z., Partridge S.R., Thomas L., Iredell J.R. Antimicrob. Agents Chemother. 52:4198-4202(2008) [PubMed: 18725449] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: JIE059 EMBL ACM04461.1.
[4]	"DNA sequence analysis of the genetic environment of various blaCTX-M genes." Eckert C., Gautier V., Arlet G. J. Antimicrob. Chemother. 57:14-23(2006) [PubMed: 16291869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: TN05 EMBL CAJ13580.1.
[5]	"Structure, function, and inhibition along the reaction coordinate of CTX-M beta-lactamases." Chen Y., Shoichet B., Bonnet R. J. Am. Chem. Soc. 127:5423-5434(2005) [PubMed: 15826180] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.12 ANGSTROMS) OF 29-291 IN COMPLEX WITH CEFOXITIN.
[6]	"The acylation mechanism of CTX-M beta-lactamase at 0.88 a resolution." Chen Y., Bonnet R., Shoichet B.K. J. Am. Chem. Soc. 129:5378-5380(2007) [PubMed: 17408273] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (0.88 ANGSTROMS) OF 30-288.
[7]	"Structural insights into substrate recognition and product expulsion in CTX-M enzymes." Delmas J., Leyssene D., Dubois D., Birck C., Vazeille E., Robin F., Bonnet R. J. Mol. Biol. 400:108-120(2010) [PubMed: 20452359] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 29-291.
[8]	"Molecular docking and ligand specificity in fragment-based inhibitor discovery." Chen Y., Shoichet B.K. Nat. Chem. Biol. 5:358-364(2009) [PubMed: 19305397] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.31 ANGSTROMS) OF 29-291.
[9]	"Atomic resolution structures of CTX-M beta-lactamases: extended spectrum activities from increased mobility and decreased stability." Chen Y., Delmas J., Sirot J., Shoichet B., Bonnet R. J. Mol. Biol. 348:349-362(2005) [PubMed: 15811373] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (0.98 ANGSTROMS) OF 29-291 IN COMPLEX WITH SUCROSE.
[10]	"Novel complex sul1-type integron in Escherichia coli carrying bla(CTX-M-9)." Sabate M., Navarro F., Miro E., Campoy S., Mirelis B., Barbe J., Prats G. Antimicrob. Agents Chemother. 46:2656-2661(2002) [PubMed: 12121950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 785-D EMBL AAF05311.2.
[11]	"Three cefotaximases, CTX-M-9, CTX-M-13, and CTX-M-14, among Enterobacteriaceae in the People's Republic of China." Chanawong A., M'Zali F.H., Heritage J., Xiong J.H., Hawkey P.M. Antimicrob. Agents Chemother. 46:630-637(2002) [PubMed: 11850241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ACH08 EMBL AAF72529.1.
[12]	Fursova N.K., Pryamchuk S.D., Abaev I.V., Shishkova N.A., Pecherskikh E.I., Korobova O.V. Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: K-340 EMBL ADJ19345.1.
[13]	Arlet G.J. Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: TN05 EMBL CAJ13580.1.
[14]	"Dynamic view of the early and late steps of the catalytic mechanism mediated by the emerging enzymes CTX-M." Delmas J., Leyssene D., Dubois D., Birck C., Samama J.-P., Robin F., Bonnet R. Submitted (JUN-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 29-291.
[15]	Arlet G.J. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: RAJ EMBL CAC95176.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF174129 Genomic DNA. Translation: AAF05311.2.
AF252621 Genomic DNA. Translation: AAF72529.1.
EU418915 Genomic DNA. Translation: ACM04461.1.
HM569735 Genomic DNA. Translation: ADJ19345.1.
AJ416345 Genomic DNA. Translation: CAC95176.1.
AM040708 Genomic DNA. Translation: CAJ13580.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YLJ	X-ray	0.98	A	29-291	[»]
1YM1	X-ray	1.12	A/B	29-291	[»]
1YMS	X-ray	1.60	A/B	29-291	[»]
1YMX	X-ray	1.70	A/B	29-291	[»]
2P74	X-ray	0.88	A/B	30-288	[»]
3G2Y	X-ray	1.31	A/B	30-291	[»]
3G2Z	X-ray	1.50	A/B	29-291	[»]
3G30	X-ray	1.80	A	29-291	[»]
3G31	X-ray	1.70	A/B	29-291	[»]
3G32	X-ray	1.31	A/B	29-291	[»]
3G34	X-ray	1.31	A/B	29-291	[»]
3G35	X-ray	1.41	A/B	29-291	[»]
3HLW	X-ray	1.50	A/B	29-291	[»]
3HRE	X-ray	1.45	A/B	29-291	[»]
3HUO	X-ray	1.50	A/B	29-291	[»]
3HVF	X-ray	1.50	A/B	29-291	[»]

ProteinModelPortal

Q9L5C8.

SMR

Q9L5C8. Positions 29-291.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]

Gene3D

G3DSA:3.40.710.10. G3DSA:3.40.710.10. 1 hit.

Pfam

PF00144. Beta-lactamase. 1 hit.
[Graphical view]

PRINTS

PR00118. BLACTAMASEA.

SUPFAM

SSF56601. PBP_transp_fold. 1 hit.

PROSITE

PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q9L5C8_ECOLX

Accession

Primary (citable) accession number: Q9L5C8
Secondary accession number(s): Q9RP34

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2000
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)