Q9L4W4 (Q9L4W4_9ACTO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
NysB EMBL AAF71775.1

Gene names

Name:

nysB EMBL AAF71775.1

Organism

Streptomyces noursei EMBL AAF71775.1

Taxonomic identifier

1971 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	3192 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Ligand	Phosphopantetheine RuleBase RU000721
Molecular function	Transferase RuleBase RU000693
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q9L4W4 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: F88F8864F086DA64	FASTA	3,192	333,922
	10 20 30 40 50 60 MQEPQQGQPD QQEKIVDYLR RVTSDLRRAR RRIGELESKD NEPIAIVGMG CRLPGGVNSP 70 80 90 100 110 120 ESLWDLVRSG GDAISGFPVD RGWDLETLTG NGDGSSATHE GGFLYDAAEF DAAFFGISPR 130 140 150 160 170 180 EATAMDPQQR LLLEVAWEAL ERAGIAPTAL RGSRSGVFVG SYHWGAPSAD AATELHGHAL 190 200 210 220 230 240 TGTAASVLSG RLAYTLGLEG PAVTVDTACS SSLVALHLAA QSLRVGESSL AVIGGVTILT 250 260 270 280 290 300 EPSVFVEFSA QGGLAPDGRC KAFSDAADGT GWAEGVGVLV AERLSDAQRN GHPVLAVLRG 310 320 330 340 350 360 SAVNQDGASN GLTAPNGPSQ ERVIQQALAR TGLTPADIDA VEAHGTGTRL GDPIEAQALL 370 380 390 400 410 420 ATYGQGHTPD QPLWLGSLKS NIGHTQAAAG VAGVIKMVMA LRHGHLPPTL HADAPSSHVD 430 440 450 460 470 480 WSAGSVRLLT EGQQWPETGR PRRAAVSSFG ISGTNAHALL EQAPHPADTA DAGDDAAPTE 490 500 510 520 530 540 PAGAPAALPW IVSGHSPQAL RDQAAALAAR VETDPALRPQ DIGHTLHTAR ALLERRAVVV 550 560 570 580 590 600 APDRAELLAA THELAAGRSA NAVVEGLADV EGRTVFVFPG QGSQWVGMGA QLLDESAVFA 610 620 630 640 650 660 ERIAECAAAL AEFTDWSLVD VLRGVVGAPS LERVDVVQPA SFAVMVSLAA LWGSRGVLPD 670 680 690 700 710 720 AVVGHSQGEI AAAVVSGALS LRDGARVVAL RSQAIGRALA GRGGMMSVAL SVDVLEPRLV 730 740 750 760 770 780 EFEGRVSVAA VNGPRSVVVA GEPEALDALH ARLTADDIRA RRIAVDYASH SHQVEDLHEE 790 800 810 820 830 840 LLEVLAELAP RTSEVPFFST VTGDWLDTAR MDAGYWFRNL RGRVRFADAV ADLLAAEYRA 850 860 870 880 890 900 FVEVSSHPVL TMAVLDLIEE AGVTAVATGT LRRDQGGAGR FLLSAAEVFV RGVDVDWAGA 910 920 930 940 950 960 FEGTGAARVD LPTYAFQRER YWNTRTAADR TPADAPMDAE FWAAVEQADV SALTAALGTD 970 980 990 1000 1010 1020 EDSVAAILPG LTSWRRARSQ RTTLDSWRYR VTWTPLAQVP RATLTGTWLL VTTDGIDDTD 1030 1040 1050 1060 1070 1080 VAGALESYGA EVRRLVLDEE CTDRAVLRER LAGAEDVTGI VSVLAAAEDD AARHPGLTRG 1090 1100 1110 1120 1130 1140 LALTVSLVQA LGDAEATAPL WFLTRGAFAT GPSDPVTRPL QSQIAGVGWT TALEHPQRWG 1150 1160 1170 1180 1190 1200 GTVDLPDTLD ARAAQRLAAA LSGALGAEDQ LAVRAAGVLA RRIVRAGHRA GRPARTWAPR 1210 1220 1230 1240 1250 1260 GTTLITGGSG TLAPQLARWL AERGAEHVVL VSRRGADAPG APELIAEAAE SGTEVTVAAC 1270 1280 1290 1300 1310 1320 DITDRDAVAA LLADLTADGR TLRTVIHAAA AIELSALADT TVAEFADVVH AKVTGARILD 1330 1340 1350 1360 1370 1380 ELLDDAELDD FVLYSSTAGM WGSGVHAAYV AGNAYLSALA EQRRARGLRT TSIHWGKWPD 1390 1400 1410 1420 1430 1440 DRARELADPH RIRRSGLEYL DPELALTALQ HVLDDDETVI GLMDIDWDTY HDVFTAGRPA 1450 1460 1470 1480 1490 1500 HLFDQIPEVR RRLDQASVPD PAGPAADGLA ARLHGLAAAE QDRLLLTLVR TEAAAVLGHA 1510 1520 1530 1540 1550 1560 SAESFPERRA FRDLGFDSVT AVDLRNRLVA GTGLRLPSTM VFDHPNCAAL AAFLKTTALG 1570 1580 1590 1600 1610 1620 VPGAAPQQHA ATGTPADDDP IAVIGMSCRY PGGAATPEEL LRLALDGADV ISEFPADRGW 1630 1640 1650 1660 1670 1680 DARGLYDPDP DRPGHTYSVQ GGFLHEAAGF DPGFFGISPR EAVAMDPQQR LLLETSWEAF 1690 1700 1710 1720 1730 1740 ERAGIDPASL RGSAAGTFFG ASYQDYSSTV QNGTGESEAH MVTGTAASVL SGRVSYLLGL 1750 1760 1770 1780 1790 1800 EGPAVTVDTA CSSSLVALHL ACQSLRDGES SLALAGGAAV MATPHAFVGF SRQRALAKDG 1810 1820 1830 1840 1850 1860 RCKPFSDTAD GMTLAEGVGV VLLERLSHAR ANGHRVLAVI RGSAVNQDGA SNGLTAPNGP 1870 1880 1890 1900 1910 1920 SQQRVIRQAL ANAGLTGADV DAVEAHGTGT KLGDPIEAQA LLATYGQDRD AERPLLLGSV 1930 1940 1950 1960 1970 1980 KSNIGHTQAA AGVAGVIKMV LAMDAGELPG TLHLDAPSSH VDWTAGAVEL LRGRTPWPES 1990 2000 2010 2020 2030 2040 GRPRRAGVSS FGISGTNAHL ILEQAPATEP PADPDRLRDT ATDTVVPWPL AAKSPAALRA 2050 2060 2070 2080 2090 2100 QAARLLATVE HDPDLPPAPV GHALATTRAA LEHRAVVVGE RREDFLRGLA ALSTGASTAG 2110 2120 2130 2140 2150 2160 LVSGIAGPDP EGAVFVFPGQ GSQWWGMGRE LLATSEVFRT AIDDCATALA PYVDWSLHDV 2170 2180 2190 2200 2210 2220 LAGEGDPALL ERVDVVQPAL FAMMVGLSAL WRSHGVVPAA VVGHSQGEIA AACVAGALSL 2230 2240 2250 2260 2270 2280 ADAARVVALR SQALPQLSGR GGMMSVSAPV ERVTALLAPW QEALSVAAVN GPSSVVVSGD 2290 2300 2310 2320 2330 2340 TDALDALHTA CQEQGVRARK VSVDYASHGR HVEAVRDELA RVLAPVDPRA PEVPFYSTVT 2350 2360 2370 2380 2390 2400 GDRVDDAAFD GAYWYTNLRQ TVRMEEATRA LLAAGHRVFI EVSPHPVLAA PIQETQEAVA 2410 2420 2430 2440 2450 2460 EATGGSAVVL GSLRRDEGGP RRFLTSLAEA HTHGAPVDWT TTFARSAYQP VDLPTYPFQR 2470 2480 2490 2500 2510 2520 QDFWPEARPA TPAAGADASD AAFWQLVENQ DLAALADALG VPADDEHTAL GTVLPALSAW 2530 2540 2550 2560 2570 2580 RAKAQARTRI DELRYHVQWT RVAEPAAAPT TGRLLVAVPP DHADAPWVAA ALDALGTDTV 2590 2600 2610 2620 2630 2640 RFEAKGTDRA GWAAQIAQLV EDGEEFTGVV SLLAAAEDLH PDFGSVPLGL GQTLVLVQAL 2650 2660 2670 2680 2690 2700 GDAGLTAPLW CLTRGAVATG RDDALDSPTQ GALWGLGRVV ALEHPDRWGG LIDLPATLDA 2710 2720 2730 2740 2750 2760 RAAARLTGLL ADPAGEDQLA VRATGVLARR MVHAAPSAPR TGRRWRGRGT CLITGGTGGI 2770 2780 2790 2800 2810 2820 GGRVARWMAE HGAAHLVLTS RRGPDAPGAA ALRAELEALG ARVTLAACDV ADRDALAALL 2830 2840 2850 2860 2870 2880 ADLPADQPLT SVFHSAGVAD GDARAADLTL DQLDALLRAK LTAAHHLHEL TAPLDLDAFV 2890 2900 2910 2920 2930 2940 LFSSGAAVWG SGGQPGYAAA NAYLDALAAH RRSLDLPGAS VAWGTWGEVG MATVPEVHER 2950 2960 2970 2980 2990 3000 LHRQGVRAME PDHAIGALQQ MLEDDDTTLA VTLMDWEAFA PSFTATRPSA LFSTVPEAVR 3010 3020 3030 3040 3050 3060 AVTGDPGTTA GDDVDSATPP LRRHLEELSA AERGRALVEA VRAEASATLG HDTPDAIPAG 3070 3080 3090 3100 3110 3120 RAFRDVGFDS VTAVELRNRL RTALGLPLPA ALVFDHPTPT ALAGHLGALL FGTAPEDAGT 3130 3140 3150 3160 3170 3180 GRPDDPDARI REALATVPIG RLRKAGLLDM VLKLADGDAT DAPAPEADAP SESLDDMDAE 3190 ALLRLATENS AN

References

[1]	"Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway." Brautaset T., Sekurova O.N., Sletta H., Ellingsen T.E., StrLm A.R., Valla S., Zotchev S.B. Chem. Biol. 7:395-403(2000) [PubMed: 10873841] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 11455 EMBL AAF71775.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF263912 Genomic DNA. Translation: AAF71775.1.
3D structure databases
HSSP	HSSP built from PDB template 1NQ4 based on UniProtKB P43677.
ProteinModelPortal	Q9L4W4.
SMR	Q9L4W4. Positions 12-923, 1467-1556, 1577-2466.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001227. Ac_transferase_dom. IPR009081. Acyl_carrier_prot-like. IPR014043. Acyl_transferase. IPR016035. Acyl_Trfase/lysoPLipase. IPR000794. Beta-ketoacyl_synthase. IPR002198. DH_sc/Rdtase_SDR. IPR018201. Ketoacyl_synth_AS. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016036. Malonyl_transacylase_ACP-bd. IPR016040. NAD(P)-bd_dom. IPR006163. Phsphopanteth-bd. IPR020842. PKS/FAS_KR. IPR020801. PKS_acyl_transferase. IPR020841. PKS_Beta-ketoAc_synthase_dom. IPR013968. PKS_KR. IPR020806. PKS_PP-bd. IPR015083. Polyketide_synth_docking. IPR006162. PPantetheine_attach_site. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.366.10. Ac_transferase_reg. 4 hits. G3DSA:1.10.1200.10. ACP_like. 2 hits. G3DSA:3.40.50.720. NAD(P)-bd. 2 hits. G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
PANTHER	PTHR11712. Ketoacyl_synth. 1 hit.
Pfam	PF00698. Acyl_transf_1. 2 hits. PF00106. adh_short. 1 hit. PF08990. Docking. 1 hit. PF00109. ketoacyl-synt. 2 hits. PF02801. Ketoacyl-synt_C. 2 hits. PF08659. KR. 1 hit. PF00550. PP-binding. 2 hits. [Graphical view]
SMART	SM00827. PKS_AT. 2 hits. SM00822. PKS_KR. 2 hits. SM00825. PKS_KS. 2 hits. SM00823. PKS_PP. 2 hits. [Graphical view]
SUPFAM	SSF47336. ACP_like. 2 hits. SSF52151. Acyl_Trfase/lysoPlipase. 2 hits. SSF55048. Malonyl_transacylase_ACP-bd. 2 hits. SSF53901. Thiolase-like. 2 hits.
PROSITE	PS50075. ACP_DOMAIN. 2 hits. PS00606. B_KETOACYL_SYNTHASE. 2 hits. PS00012. PHOSPHOPANTETHEINE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q9L4W4_9ACTO

Accession

Primary (citable) accession number: Q9L4W4

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2000
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)