Q9L4T8 (PYRB_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	EF_1719

Organism

Enterococcus faecalis (Streptococcus faecalis)

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_0000113133

Sequences

	Sequence		Length	Mass (Da)
	Q9L4T8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3472C9C8780C3624	FASTA	308	34,980
	10 20 30 40 50 60 MIITSERISL KHLLTAEALT DREVMGLIRR AGEFKQGAKW HPEERQYFAT NLFFENSTRT 70 80 90 100 110 120 HKSFEVAEKK LGLEVIEFEA SRSSVQKGET LYDTVLTMSA IGVDVAVIRH GKENYYDELI 130 140 150 160 170 180 QSKTIQCSII NGGDGSGQHP TQCLLDLMTI YEEFGGFEGL KVAIVGDITH SRVAKSNMQL 190 200 210 220 230 240 LNRLGAEIYF SGPEEWYDHQ FDVYGQYVPL DEIVEKVDVM MLLRVQHERH DGKESFSKEG 250 260 270 280 290 300 YHLEYGLTNE RATRLQKHAI IMHPAPVNRD VELADELVES LQSRIVAQMS NGVFMRMAIL EAILHGKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Enterococcus faecalis pyrimidine biosynthetic gene (pyrB) encoding aspartate transcarbamoylase." Cooke P.A., Shanley M.S., O'Donovan G.A. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 19433 / DSM 20478 / JCM 8726 / NBRC 100481 / NCIMB 775.
[2]	"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700802 / V583.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF264709 Genomic DNA. Translation: AAF72727.1. AE016830 Genomic DNA. Translation: AAO81495.1.
RefSeq	NP_815425.1. NC_004668.1.
3D structure databases
ProteinModelPortal	Q9L4T8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1200611.
GenomeReviews	Gene locus EF_1719 in contig AE016830_GR.
KEGG	efa:EF1719.
NMPDR	fig|226185.1.peg.1610.
PATRIC	21853782. VBIEntFae7065_1617.
TIGR	EF_1719.
Phylogenomic databases
HOGENOM	HBG579429.
OMA	VGIPIIN.
ProtClustDB	PRK00856.
Enzyme and pathway databases
BioCyc	EFAE226185:EF_1719-MONOMER.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_ENTFA

Accession

Primary (citable) accession number: Q9L4T8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program