Reviewed, UniProtKB/Swiss-Prot Q9L4N5 (CARA_LACLM)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	llmg_0894

Organism

Lactococcus lactis subsp. cremoris (strain MG1363) [Complete proteome] [HAMAP]

Taxonomic identifier

416870 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112285
Regions
	Domain	172 – 357	186	Glutamine amidotransferase type-1
	Region	1 – 168	168	CPSase HAMAP MF_01209
Sites
	Active site	247	1	Nucleophile By similarity
	Active site	331	1	By similarity
	Active site	333	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9L4N5-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 0F12E0A267014757	FASTA	357	39,637
	10 20 30 40 50 60 MSKRLLILED GTIFEGEALG ANLDVTGELV FNTGMTGYQE SITDQSYNGQ ILTFTYPIVG 70 80 90 100 110 120 NYGVNRDDYE SIHPTCKAVV VHEAARRPSN WRMQMSFDEF LKSKNIPGIT GVDTRAITKI 130 140 150 160 170 180 VREHGTMKAS LVQARDEVDH QMSQLQATVL PTNQVETSST ATAYPSPNTG RKVVVVDFGL 190 200 210 220 230 240 KHSILRELSK RECNLTVVPY NTSAKEILEM EPDGVMLTNG PGDPTDVPEA IEMIKEVQGK 250 260 270 280 290 300 IPIFGICLGH QLFSLANGAT TYKMKFGHRG FNHAVREVAT GRIDFTSQNH GYAVSSENLP 310 320 330 340 350 EDLMITHVEI NDNSVEGVRH KYFPAFSVQF HPDAAPGPHD ASYLFDDFMD LMDNFKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The pyrimidine operon pyrRPB-carA from Lactococcus lactis." Martinussen J., Schallert J., Andersen B., Hammer K. J. Bacteriol. 183:2785-2794(2001) [PubMed: 11292797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363." Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J. J. Bacteriol. 189:3256-3270(2007) [PubMed: 17307855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ132624 Genomic DNA. Translation: CAB89872.1. AM406671 Genomic DNA. Translation: CAL97488.1.
RefSeq	YP_001032220.1.
3D structure databases
HSSP	HSSP built from PDB template 1JDB based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	4798479.
GenomeReviews	Gene locus llmg_0894 in contig AM406671_GR.
KEGG	llm:llmg_0894.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9L4N5. LFDGSNC.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_LACLM

Accession

Primary (citable) accession number: Q9L4N5
Secondary accession number(s): A2RJN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)