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Q9L4K1 (BETB_CHRSD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD/NADP-dependent betaine aldehyde dehydrogenase

Short name=BADH
EC=1.2.1.8
Gene names
Name:betB
OrganismChromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Taxonomic identifier290398 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHalomonadaceaeChromohalobacter

Protein attributes

Sequence length489 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid By similarity. HAMAP-Rule MF_00804

Catalytic activity

Betaine aldehyde + NAD+ + H2O = betaine + NADH. HAMAP-Rule MF_00804

Cofactor

Binds 2 potassium ions per subunit By similarity. HAMAP-Rule MF_00804

Pathway

Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine from betaine aldehyde: step 1/1. HAMAP-Rule MF_00804

Subunit structure

Dimer of dimers By similarity. HAMAP-Rule MF_00804

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 489489NAD/NADP-dependent betaine aldehyde dehydrogenase HAMAP-Rule MF_00804
PRO_0000056545

Regions

Nucleotide binding150 – 1534NAD/NADP By similarity
Nucleotide binding176 – 1794NAD/NADP By similarity
Nucleotide binding228 – 2336NAD/NADP By similarity

Sites

Active site1621Charge relay system By similarity
Active site2511Proton acceptor By similarity
Active site4631Charge relay system By similarity
Metal binding931Potassium 1 By similarity
Metal binding1801Potassium 1; via carbonyl oxygen By similarity
Metal binding2451Potassium 2; via carbonyl oxygen By similarity
Metal binding4561Potassium 2; via carbonyl oxygen By similarity
Metal binding4591Potassium 2; via carbonyl oxygen By similarity
Binding site2091NAD/NADP; via amide nitrogen By similarity
Binding site2851NAD/NADP By similarity
Binding site3861NAD/NADP By similarity
Site2471Seems to be a necessary countercharge to the potassium cations By similarity

Amino acid modifications

Modified residue2851Cysteine sulfenic acid (-SOH) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9L4K1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 42AFE892DA57DD4B

FASTA48952,333
        10         20         30         40         50         60 
MTAQSAQPLY IGGRPVDATS GETFTVTNPY DGSLLATIGQ ASQADVDSAV QAAQRGQREW 

        70         80         90        100        110        120 
AAMTGMERSR ILLRAVALLR ERNDELAELE TRNTGKPISE TASVDIVTGA DALEYYAGLA 

       130        140        150        160        170        180 
PAIEGSQIPL RDTRLVYTRR EPLGVIGAIG AWNYPIQIAC WKAAPALAAG NAIVFKPSEV 

       190        200        210        220        230        240 
TPLTALKLAE IFTEAGLPDG VFNVVQGDGR VGAMLTEHEG IAKVSFTGEV GTGKKVMAAA 

       250        260        270        280        290        300 
GGSTLKDVTM ELGGKSPLIV FADADLDRAA DAAMMANFYS SGQICTNGTR VFVERSAKEA 

       310        320        330        340        350        360 
FEAKLVERVA RIKAGDPMDP SVNFGPLVSF EHQEKVLSYI ALGKEQGARV LAGGDAWNSG 

       370        380        390        400        410        420 
EWAKGAWAAP TVFTDCTDEM RVVKEEIFGP VMSVLAFDDE EEVIRRANNT KYGLAAGVFS 

       430        440        450        460        470        480 
ESLNRAHRVI HQLEAGICWI NTWGESPSEM PVGGYKESGI GRENGVETLN HYTQTKSVQI 


EMGPFESVF 

« Hide

References

[1]"Genes for the synthesis of the osmoprotectant glycine betaine from choline in the moderately halophilic bacterium Halomonas elongata DSM 3043."
Canovas D., Vargas C., Kneip S., Moron M.J., Ventosa A., Bremer E., Nieto J.J.
Microbiology 146:455-463(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ238780 Genomic DNA. Translation: CAB77175.1.

3D structure databases

ProteinModelPortalQ9L4K1.
SMRQ9L4K1. Positions 8-489.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00529; UER00386.

Family and domain databases

Gene3D3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPMF_00804. BADH.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. SSF53720. 1 hit.
TIGRFAMsTIGR01804. BADH. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBETB_CHRSD
AccessionPrimary (citable) accession number: Q9L4K1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways