Reviewed, UniProtKB/Swiss-Prot Q9L4K0 (BETA2_CHRSD)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:

betA

Organism

Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)

Taxonomic identifier

290398 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Halomonadaceae › Chromohalobacter

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine. HAMAP MF_00750
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity. HAMAP MF_00750
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Choline dehydrogenase HAMAP MF_00750		PRO_0000205589
Regions
	Nucleotide binding	8 – 37	30	FAD Probable
Sites
	Active site	475	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9L4K0-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: DE85CD0E7FEF2F14	FASTA	558	62,083
	10 20 30 40 50 60 MSQATEFDYI IIGAGSAGNV LATRLTEDSD VSVLLLEAGG PDYRFDFRTQ MPAALAYPLQ 70 80 90 100 110 120 GKRYNWAFET DPEPHMDNRR MECGRGKGLG GSSLINGMCY IRGNALDYDH WAKQPGLEEW 130 140 150 160 170 180 DYLSCLPYFK KSETRDIGPN DYHGGDGPVS VTTPKAGNNP LYRTFIEAGK QAGYPETEDV 190 200 210 220 230 240 NGYQQEGFGP MDRFVTPKGR RASTARGYLD TAKQRSNLTI ETRAVTDVIE FEGKRAVGVR 250 260 270 280 290 300 YEQKGQPKQA RARREVLLCG GAIASPQILQ RSGVGNPEWL KELGIPVVHE LPGVGENLQD 310 320 330 340 350 360 HLEMYIQYEC KEPISLYPAL KWYNQPKIGA EWLFKGTGVG ASNQFESCGF IRSRDDEEWP 370 380 390 400 410 420 NLQYHFLPIA ISYNGKSAVQ AHGFQAHVGS MRSESRGRIR LTSKDPHAAP SILFNYMAKE 430 440 450 460 470 480 KDWEEFRDAI RLTREIIAQP AFDRYRGREI SPGPDVQSDE ELDNFVKQHA ETAYHPCGSC 490 500 510 520 530 540 RMGEGDMAVT DAQGRVHGLE GLRVVDASLF PVIPTGNLNA PTIMLAEKIA DRIRGREPLP 550 RASVDYYVAN GAPAKQAS

References

[1]

"Genes for the synthesis of the osmoprotectant glycine betaine from choline in the moderately halophilic bacterium Halomonas elongata DSM 3043." Canovas D., Vargas C., Kneip S., Moron M.J., Ventosa A., Bremer E., Nieto J.J. Microbiology 146:455-463(2000) [PubMed: 10708384] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ238780 Genomic DNA. Translation: CAB77176.1.
3D structure databases
SMR	Q9L4K0. Positions 5-533.
ModBase	Search...
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA2_CHRSD

Accession

Primary (citable) accession number: Q9L4K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)