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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7MagnesiumBy similarity1
Metal bindingi68MagnesiumBy similarity1
Metal bindingi141MagnesiumBy similarity1
Metal bindingi144MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:SCO1520
ORF Names:SCL2.10c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001831351 – 188Crossover junction endodeoxyribonuclease RuvCAdd BLAST188

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO1520

Structurei

3D structure databases

ProteinModelPortaliQ9L289
SMRiQ9L289
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.Curated

Phylogenomic databases

eggNOGiENOG4105NHU Bacteria
COG0817 LUCA
HOGENOMiHOG000012182
InParanoidiQ9L289
KOiK01159
OMAiGLTRCGI
OrthoDBiPOG091H0202
PhylomeDBiQ9L289

Family and domain databases

Gene3Di3.30.420.10, 1 hit
HAMAPiMF_00034 RuvC, 1 hit
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR020563 X-over_junc_endoDNase_Mg_BS
IPR002176 X-over_junc_endoDNase_RuvC
PANTHERiPTHR30194 PTHR30194, 1 hit
PfamiView protein in Pfam
PF02075 RuvC, 1 hit
PRINTSiPR00696 RSOLVASERUVC
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00228 ruvC, 1 hit
PROSITEiView protein in PROSITE
PS01321 RUVC, 1 hit

Sequencei

Sequence statusi: Complete.

Q9L289-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLGVDPGL TRRGIGVVEG VAGRPLTMIG VGVVRTPADA DLGHRLVAVE
60 70 80 90 100
QGIEQWLDEH RPEFVAVERV FSQHNVRTVM GTAQASAVAI LCASRRGIPV
110 120 130 140 150
ALHTPSEVKA AVTGSGRADK AQVGAMVTRL LRLAAPPKPA DAADALALAI
160 170 180
CHIWRAPAQN RLQQAVALHT AQGPRRPHKL HPSKGRPA
Length:188
Mass (Da):19,890
Last modified:October 1, 2000 - v1
Checksum:iB19A98CF96A64B9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939109 Genomic DNA Translation: CAB70922.1
RefSeqiNP_625799.1, NC_003888.3
WP_011027826.1, NC_003888.3

Genome annotation databases

EnsemblBacteriaiCAB70922; CAB70922; CAB70922
GeneIDi1096946
KEGGisco:SCO1520
PATRICifig|100226.15.peg.1529

Similar proteinsi

Entry informationi

Entry nameiRUVC_STRCO
AccessioniPrimary (citable) accession number: Q9L289
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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