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Protein

ATP-dependent 6-phosphofructokinase 2

Gene

pfkA2

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation1 Publication

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 Publication

Enzyme regulationi

Allosterically inhibited by phosphoenolpyruvate.1 Publication

Kineticsi

  1. KM=0.4 mM for ATP1 Publication
  1. Vmax=165 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 7.5-8.1 Publication

Temperature dependencei

Optimum temperature is 30-50 degrees Celsius.1 Publication

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase 2 (pgi2), Glucose-6-phosphate isomerase 1 (pgi1)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfkA1), ATP-dependent 6-phosphofructokinase 2 (pfkA2), ATP-dependent 6-phosphofructokinase 3 (pfkA3)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10ATP; via amide nitrogenUniRule annotation1
Metal bindingi103Magnesium; catalyticUniRule annotation1
Sitei104Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei127Proton acceptorUniRule annotation1
Binding sitei162Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei222SubstrateUniRule annotation1
Binding sitei266Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 73ATPUniRule annotation2
Nucleotide bindingi102 – 105ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.11. 5998.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 2UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 2UniRule annotation
Short name:
Phosphofructokinase 2UniRule annotation
Alternative name(s):
Phosphohexokinase 2UniRule annotation
Gene namesi
Name:pfkA2UniRule annotation
Synonyms:pfk2
Ordered Locus Names:SCO5426
ORF Names:SC6A11.02
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Accumulates glucose 6-phosphate and fructose 6-phosphate. Has an increased carbon flux through the pentose phosphate pathway, resulting in a higher production of the pigmented antibiotics actinorhodin and undecylprodigiosin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119871 – 341ATP-dependent 6-phosphofructokinase 2Add BLAST341

Proteomic databases

PRIDEiQ9L1L8.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO5426.

Structurei

3D structure databases

ProteinModelPortaliQ9L1L8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 127Substrate bindingUniRule annotation3
Regioni169 – 171Substrate bindingUniRule annotation3
Regioni272 – 275Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248869.
InParanoidiQ9L1L8.
KOiK00850.
OMAiVMGHEAG.
OrthoDBiPOG091H01AC.
PhylomeDBiQ9L1L8.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9L1L8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGVLTAGG DCPGLNAVIR SVVHRAVDNY GDEVIGFEDG YAGLLDGRYR
60 70 80 90 100
ALDLNAVSGI LARGGTILGS SRLERDRLRE ACENAGDMIQ NFGIDALIPI
110 120 130 140 150
GGEGTLTAAR MLSDAGLPVV GVPKTIDNDI SSTDRTFGFD TAVGVATEAM
160 170 180 190 200
DRLKTTAESH QRVMVVEVMG RHAGWIALES GMAAGAHGIC LPERPFDPAD
210 220 230 240 250
LVKMVEERFS RGKKFAVVCV AEGAHPAEGS MDYGKGAIDK FGHERFQGIG
260 270 280 290 300
TALAFELERR LGKEAKPVIL GHVQRGGVPT AYDRVLATRF GWHAVEAAHR
310 320 330 340
GDFGRMTALR GTDVVMVPLA EAVTELKTVP KDRMDEAESV F
Length:341
Mass (Da):36,434
Last modified:October 1, 2000 - v1
Checksum:i6C0B09B76FE90BBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939123 Genomic DNA. Translation: CAB72402.1.
RefSeqiNP_629564.1. NC_003888.3.
WP_003973572.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB72402; CAB72402; CAB72402.
GeneIDi1100866.
KEGGisco:SCO5426.
PATRICi23740702. VBIStrCoe124346_5507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939123 Genomic DNA. Translation: CAB72402.1.
RefSeqiNP_629564.1. NC_003888.3.
WP_003973572.1. NC_003888.3.

3D structure databases

ProteinModelPortaliQ9L1L8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO5426.

Proteomic databases

PRIDEiQ9L1L8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB72402; CAB72402; CAB72402.
GeneIDi1100866.
KEGGisco:SCO5426.
PATRICi23740702. VBIStrCoe124346_5507.

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248869.
InParanoidiQ9L1L8.
KOiK00850.
OMAiVMGHEAG.
OrthoDBiPOG091H01AC.
PhylomeDBiQ9L1L8.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BRENDAi2.7.1.11. 5998.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA2_STRCO
AccessioniPrimary (citable) accession number: Q9L1L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The nucleotide sequence reported in PubMed:9055413 was not that of the characterized enzyme. Inspection of the original N-terminal sequence showed that the characterized enzyme was pfkA2 (SCO5426) and not pfkA1 (SCO2119), another isozyme in S.coelicolor (PubMed:18606812).1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.