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Q9L1L8

- PFKA2_STRCO

UniProt

Q9L1L8 - PFKA2_STRCO

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Protein
ATP-dependent 6-phosphofructokinase 2
Gene
pfkA2, pfk2, SCO5426, SC6A11.02
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.1 Publication

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 Publication

Cofactori

Magnesium.1 Publication

Enzyme regulationi

Allosterically inhibited by phosphoenolpyruvate.1 Publication

Kineticsi

  1. KM=0.4 mM for ATP1 Publication

Vmax=165 µmol/min/mg enzyme

pH dependencei

Optimum pH is 7.5-8.

Temperature dependencei

Optimum temperature is 30-50 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATP; via amide nitrogen By similarity
Metal bindingi103 – 1031Magnesium; catalytic By similarity
Sitei104 – 1041Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity
Active sitei127 – 1271Proton acceptor By similarity
Binding sitei162 – 1621Substrate; shared with dimeric partner By similarity
Binding sitei222 – 2221Substrate By similarity
Binding sitei266 – 2661Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATP By similarity
Nucleotide bindingi102 – 1054ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 2 (EC:2.7.1.11)
Short name:
ATP-PFK 2
Short name:
Phosphofructokinase 2
Alternative name(s):
Phosphohexokinase 2
Gene namesi
Name:pfkA2
Synonyms:pfk2
Ordered Locus Names:SCO5426
ORF Names:SC6A11.02
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Accumulates glucose 6-phosphate and fructose 6-phosphate. Has an increased carbon flux through the pentose phosphate pathway, resulting in a higher production of the pigmented antibiotics actinorhodin and undecylprodigiosin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341ATP-dependent 6-phosphofructokinase 2UniRule annotation
PRO_0000111987Add
BLAST

Proteomic databases

PRIDEiQ9L1L8.

Interactioni

Subunit structurei

Homodimer or homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO5426.

Structurei

3D structure databases

ProteinModelPortaliQ9L1L8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1273Substrate binding By similarity
Regioni169 – 1713Substrate binding By similarity
Regioni272 – 2754Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiRGDFGRM.
OrthoDBiEOG644ZRM.
PhylomeDBiQ9L1L8.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9L1L8-1 [UniParc]FASTAAdd to Basket

« Hide

MRIGVLTAGG DCPGLNAVIR SVVHRAVDNY GDEVIGFEDG YAGLLDGRYR    50
ALDLNAVSGI LARGGTILGS SRLERDRLRE ACENAGDMIQ NFGIDALIPI 100
GGEGTLTAAR MLSDAGLPVV GVPKTIDNDI SSTDRTFGFD TAVGVATEAM 150
DRLKTTAESH QRVMVVEVMG RHAGWIALES GMAAGAHGIC LPERPFDPAD 200
LVKMVEERFS RGKKFAVVCV AEGAHPAEGS MDYGKGAIDK FGHERFQGIG 250
TALAFELERR LGKEAKPVIL GHVQRGGVPT AYDRVLATRF GWHAVEAAHR 300
GDFGRMTALR GTDVVMVPLA EAVTELKTVP KDRMDEAESV F 341
Length:341
Mass (Da):36,434
Last modified:October 1, 2000 - v1
Checksum:i6C0B09B76FE90BBD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939123 Genomic DNA. Translation: CAB72402.1.
RefSeqiNP_629564.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB72402; CAB72402; CAB72402.
GeneIDi1100866.
KEGGisco:SCO5426.
PATRICi23740702. VBIStrCoe124346_5507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939123 Genomic DNA. Translation: CAB72402.1 .
RefSeqi NP_629564.1. NC_003888.3.

3D structure databases

ProteinModelPortali Q9L1L8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO5426.

Proteomic databases

PRIDEi Q9L1L8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB72402 ; CAB72402 ; CAB72402 .
GeneIDi 1100866.
KEGGi sco:SCO5426.
PATRICi 23740702. VBIStrCoe124346_5507.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248869.
KOi K00850.
OMAi RGDFGRM.
OrthoDBi EOG644ZRM.
PhylomeDBi Q9L1L8.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_01976. Phosphofructokinase_III.
InterProi IPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  2. "Identification of ATP-dependent phosphofructokinase as a regulatory step in the glycolytic pathway of the actinomycete Streptomyces coelicolor A3(2)."
    Alves A.M.C.R., Euverink G.J.W., Bibb M.J., Dijkhuizen L.
    Appl. Environ. Microbiol. 63:956-961(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE OF 1-28, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, ENZYME REGULATION.
    Strain: A3(2) / 1109.
  3. "Antibiotic overproduction in Streptomyces coelicolor A3(2) mediated by phosphofructokinase deletion."
    Borodina I., Siebring J., Zhang J., Smith C.P., van Keulen G., Dijkhuizen L., Nielsen J.
    J. Biol. Chem. 283:25186-25199(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPFKA2_STRCO
AccessioniPrimary (citable) accession number: Q9L1L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The nucleotide sequence reported in 1 Publication was not that of the characterized enzyme. Inspection of the original N-terminal sequence showed that the characterized enzyme was pfkA2 (SCO5426) and not pfkA1 (SCO2119), another isozyme in S.coelicolor (1 Publication).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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