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Protein

Purine nucleoside phosphorylase

Gene

SCO3188

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Purine nucleoside phosphorylase involved in purine salvage.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151PhosphateUniRule annotation
Binding sitei194 – 1941Substrate; via amide nitrogenUniRule annotation
Binding sitei195 – 1951PhosphateUniRule annotation
Sitei231 – 2311Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
  3. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro

GO - Biological processi

  1. purine ribonucleoside salvage Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
Short name:
PNPUniRule annotation
Gene namesi
Ordered Locus Names:SCO3188
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280Purine nucleoside phosphorylasePRO_0000415083Add
BLAST

Proteomic databases

PRIDEiQ9KYV7.

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO3188.

Structurei

3D structure databases

ProteinModelPortaliQ9KYV7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni55 – 562Phosphate bindingUniRule annotation
Regioni218 – 2203Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000228987.
InParanoidiQ9KYV7.
KOiK00772.
OMAiGCDIVGM.
OrthoDBiEOG6KHFXC.
PhylomeDBiQ9KYV7.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KYV7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANKVKAEIG VIGGSGFYSF LDDVTEVRVD TPYGPPSDSL FLGEVAGRRV
60 70 80 90 100
AFLPRHGRGH HLPPHRINYR ANLWALRSVG ARQVLGPCAV GGLRPEYGPG
110 120 130 140 150
TLLVPDQFVD RTRSRPSTYF DGLPMPDGTV PNVVHVSLAD PYCPTGRAAA
160 170 180 190 200
LKAARGREWE PVDGGTLVVV EGPRFGTRAE SLWHRAQGWS VVGMTGHPEA
210 220 230 240 250
ALARELELCY TSLTLVTDLD AGAESGEGVS HEEVLRVFAA NVDRLRGVLF
260 270 280
DAVAALPASG ERDCPCGAAL GGMDPGIALP
Length:280
Mass (Da):29,755
Last modified:October 1, 2000 - v1
Checksum:iCA99954C27D27D8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939115 Genomic DNA. Translation: CAB90972.1.
RefSeqiNP_627402.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB90972; CAB90972; CAB90972.
GeneIDi1098622.
KEGGisco:SCO3188.
PATRICi23736118. VBIStrCoe124346_3248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939115 Genomic DNA. Translation: CAB90972.1.
RefSeqiNP_627402.1. NC_003888.3.

3D structure databases

ProteinModelPortaliQ9KYV7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO3188.

Proteomic databases

PRIDEiQ9KYV7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB90972; CAB90972; CAB90972.
GeneIDi1098622.
KEGGisco:SCO3188.
PATRICi23736118. VBIStrCoe124346_3248.

Phylogenomic databases

HOGENOMiHOG000228987.
InParanoidiQ9KYV7.
KOiK00772.
OMAiGCDIVGM.
OrthoDBiEOG6KHFXC.
PhylomeDBiQ9KYV7.

Enzyme and pathway databases

UniPathwayiUPA00606.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiPNPH_STRCO
AccessioniPrimary (citable) accession number: Q9KYV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it lacks several conserved amino acids in the substrate binding pocket that confer specificity towards MTA.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.