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Protein

Chorismate synthase

Gene

aroC

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.UniRule annotation

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.UniRule annotation

Cofactori

FMNH2UniRule annotationNote: Reduced FMN (FMNH(2)).UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (SCO3210)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. no protein annotated in this organism
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase 1 (aroA1), 3-phosphoshikimate 1-carboxyvinyltransferase 2 (aroA2)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421NADPUniRule annotation
Binding sitei48 – 481NADPUniRule annotation
Binding sitei302 – 3021FMN; via amide nitrogenUniRule annotation
Binding sitei343 – 3431FMNUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi137 – 1393FMNUniRule annotation
Nucleotide bindingi258 – 2592FMNUniRule annotation
Nucleotide bindingi317 – 3215FMNUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthaseUniRule annotation (EC:4.2.3.5UniRule annotation)
Short name:
CSUniRule annotation
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyaseUniRule annotation
Gene namesi
Name:aroCUniRule annotation
Synonyms:aroF
Ordered Locus Names:SCO1496
ORF Names:SC9C5.20c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Chorismate synthasePRO_0000140653Add
BLAST

Proteomic databases

PRIDEiQ9KXQ4.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO1496.

Structurei

3D structure databases

ProteinModelPortaliQ9KXQ4.
SMRiQ9KXQ4. Positions 4-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060334.
InParanoidiQ9KXQ4.
KOiK01736.
OMAiADPLRCF.
OrthoDBiPOG091H00EG.
PhylomeDBiQ9KXQ4.

Family and domain databases

CDDicd07304. Chorismate_synthase. 1 hit.
Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth. 1 hit.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 2 hits.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KXQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLRWLTAG ESHGPALVAT LEGLPAGVPI TTEMVADHLA RRRLGYGRGA
60 70 80 90 100
RMKFERDEVT FLGGVRHGLT MGSPVAVMVG NTEWPKWEQV MAADPVDPEV
110 120 130 140 150
LAGLARNAPL TRPRPGHADL AGMQKYGFDE ARPILERASA RETAARVALG
160 170 180 190 200
AVARSYLKET AGVEIVSHVV ELASAKAPHG VYPTPADVER LDADPVRCLD
210 220 230 240 250
ADASKAMVAE IDQAHKDGDT LGGVVEVLAY GVPVGLGSHV HWDRKLDARL
260 270 280 290 300
AGALMGIQAI KGVEIGDGFE LARVPGSRAH DEIVGTPDGI RRVSGRSGGT
310 320 330 340 350
EGGLTTGELL RVRAAMKPIA TVPRALKTVD VATGEAAQAH HQRSDVSAVP
360 370 380 390
AAGIVAEAMV ALVLADAVAE KFGGDSVTET RRNVRSYLDN LDIR
Length:394
Mass (Da):41,577
Last modified:October 1, 2000 - v1
Checksum:i4603A034A35174B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939109 Genomic DNA. Translation: CAB93376.1.
RefSeqiNP_625776.1. NC_003888.3.
WP_003977330.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB93376; CAB93376; CAB93376.
GeneIDi1096922.
KEGGisco:SCO1496.
PATRICi23732568. VBIStrCoe124346_1505.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939109 Genomic DNA. Translation: CAB93376.1.
RefSeqiNP_625776.1. NC_003888.3.
WP_003977330.1. NC_003888.3.

3D structure databases

ProteinModelPortaliQ9KXQ4.
SMRiQ9KXQ4. Positions 4-387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO1496.

Proteomic databases

PRIDEiQ9KXQ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB93376; CAB93376; CAB93376.
GeneIDi1096922.
KEGGisco:SCO1496.
PATRICi23732568. VBIStrCoe124346_1505.

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060334.
InParanoidiQ9KXQ4.
KOiK01736.
OMAiADPLRCF.
OrthoDBiPOG091H00EG.
PhylomeDBiQ9KXQ4.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090.

Family and domain databases

CDDicd07304. Chorismate_synthase. 1 hit.
Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth. 1 hit.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 2 hits.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROC_STRCO
AccessioniPrimary (citable) accession number: Q9KXQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.