Q9KXP9 (SYA_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	SCO1501
ORF Names:	SC9C5.25c

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	890 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 890	890	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000075224
Sites
	Metal binding	573	1	Zinc Potential
	Metal binding	577	1	Zinc Potential
	Metal binding	675	1	Zinc Potential
	Metal binding	679	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9KXP9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 05B2FD563D35F4DF	FASTA	890	95,786
	10 20 30 40 50 60 MESAEIRRRW LSFFEERGHT VVPSASLIAD DPTLLLVPAG MVPFKPYFLG EVKPPFDRAT 70 80 90 100 110 120 SVQKCVRTPD IEEVGKTTRH GTFFQMCGNF SFGDYFKEGA ITYAWELLTS AQDKGGYGLD 130 140 150 160 170 180 PERLWITVYQ DDDEAERIWH DVVGVPSERI QRLGKKDNYW SMGVPGPCGP CSEINYDRGP 190 200 210 220 230 240 EFGVEGGPAV NDERYVEIWN LVFMQYERGE GIGKEEFEIL GELPSKNIDT GLGLERLAMI 250 260 270 280 290 300 LQGVQNMYEI DTSMAVINKA TELTGVAYGD AQASDVSLRV VTDHMRTSVM LIGDGVTPGN 310 320 330 340 350 360 EGRGYVLRRI MRRAIRNMRL LGATGPVVKD LIDVVIGMMG QQYPELVTDR ERIEKVALAE 370 380 390 400 410 420 ENAFLKTLKA GTNILDTAVS DTKASGGTVL AGDKAFLLHD TWGFPIDLTL EMAAEQGLSV 430 440 450 460 470 480 DEDGFRRLMK EQRERAKADA QAKKTGHAGA GAYREIADKA GATDFVGYTD TESESTIVGI 490 500 510 520 530 540 LVDGASSPAA TEGDEVEIVL DRTPFYAEGG GQIGDTGRIR VDTGAVIEVR DCQKPVPGVY 550 560 570 580 590 600 VHKGVVQVGE VTVGAKAQAS IDDRRRKAIA RAHSATHLTH QALRDALGPT AAQAGSENQP 610 620 630 640 650 660 GRFRFDFGSP SAVPTAVMTD VEQKINEVLA RDLDVRADVM GIDEAKKQGA IAEFGEKYGE 670 680 690 700 710 720 RVRVVTIGDF SKELCGGTHV HNTAQLGLVK LLGESSIGSG VRRIEALVGV DAYSFLAREH 730 740 750 760 770 780 TVVAQLQELI KGRPEELPEK VSAMLGKLKD AEKEIEKFRA EKVLQAAGGL ADSAKDVRGV 790 800 810 820 830 840 ALVTGQVPDG TTADDLRKLV LDVRGRIQGG RAAVVALFTT VNGKPLTVIA TNEAARERGL 850 860 870 880 890 KAGDLVRTAA KTLGGGGGGK PDVAQGGGQN AAAIGDAVDA VERLVADTAK

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL939109 Genomic DNA. Translation: CAB93381.1.
RefSeq	NP_625781.1. NC_003888.3.
3D structure databases
ProteinModelPortal	Q9KXP9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1096927.
GenomeReviews	Gene locus SCO1501 in contig AL645882_GR.
KEGG	sco:SCO1501.
NMPDR	fig|100226.1.peg.1468.
PATRIC	23732578. VBIStrCoe124346_1510.
Phylogenomic databases
HOGENOM	HBG354397.
OMA	GESKTDQ.
PhylomeDB	Q9KXP9.
ProtClustDB	PRK00252.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_STRCO

Accession

Primary (citable) accession number: Q9KXP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program