Reviewed, UniProtKB/Swiss-Prot Q9KX73 (XYOA_STRSI)

Names and origin

Protein names

Recommended name:
    Xylitol oxidase
    EC=1.1.3.41
Alternative name(s):
    Alditol oxidase

Gene names

Name:

xyoA

Organism

Streptomyces sp. (strain IKD472 / FERM P-14339)

Taxonomic identifier

268810 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidation of xylitol to xylose, also acts on D-sorbitol.
Catalytic activity	An alditol + O2 = an aldose + H2O2.
Cofactor	FAD.
Subunit structure	Monomer Probable.
Sequence similarities	Belongs to the oxygen-dependent FAD-linked oxidoreductase family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	D-arabinono-1,4-lactone oxidase activity Inferred from electronic annotation. Source: InterPro FAD binding Inferred from electronic annotation. Source: InterPro xylitol oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Xylitol oxidase		PRO_0000128173
Regions
	Domain	12 – 179	168	FAD-binding PCMH-type
Amino acid modifications
	Modified residue	46	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KX73-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A9550A1B779B6816	FASTA	415	44,732
	10 20 30 40 50 60 MSTAVTNWAG NITYTAKEVH RPATAEELAD VVARSAWGAC AGAAGHSFNE IADPGPDGVL 70 80 90 100 110 120 LRLDALPAET DVDTTARTVR VGGGVRYAEL ARVVHAHGLA LPNMASLPHI SVAGSVATGT 130 140 150 160 170 180 HGSGVTNGPL AAPVREVELV TADGSQVRIA PGERRFGGAV TSLGALGVVT ALTLDLEPAF 190 200 210 220 230 240 EVGQHLFTEL PLRGLDFETV AAAGYSVSLF TDWREPGFRQ VWLKRRTDQE LPDFPWARPA 250 260 270 280 290 300 TVALHPVPGM PAENCTQQFG VPGPWHERLP HFRAEFTPSS GAELQSEYLL PRAHALDALD 310 320 330 340 350 360 AVDRIRDTVA PVLQTCEVRT VAPDEQWLGP SHGRDTVALH FTWVKDTEAV LPVVRRLEEA 370 380 390 400 410 LDAFDPRPHW GKVFTTSAAA LRARYPRLAD FRALARELDP SGKFTNTFLR DLLDG

References

[1]

"Isolation, characterization, and molecular cloning of a thermostable xylitol oxidase from Streptomyces sp. IKD472." Yamashita M., Omura H., Okamoto E., Furuya Y., Yabuuchi M., Fukahi K., Murooka Y. J. Biosci. Bioeng. 89:350-360(2000) [PubMed: 16232758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-32 AND 226-239, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D89822 Genomic DNA. Translation: BAA95146.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR007173. ALO. IPR016166. FAD-bd_2. IPR016168. FAD-linked_Oxase_FAD-bd_sub2. IPR006094. Oxid_FAD_bind_N. IPR006093. Oxy_OxRdtase_FAD_BS. [Graphical view]
Gene3D	G3DSA:3.30.465.20. FAD-linked_oxidase_FAD-bd_sub2. 1 hit.
Pfam	PF04030. ALO. 1 hit. PF01565. FAD_binding_4. 1 hit. [Graphical view]
PROSITE	PS51387. FAD_PCMH. 1 hit. PS00862. OX2_COVAL_FAD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYOA_STRSI

Accession

Primary (citable) accession number: Q9KX73

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	October 1, 2000
Last modified:	March 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)