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Q9KX40

- ESTB_BURGA

UniProt

Q9KX40 - ESTB_BURGA

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Protein

Esterase EstB

Gene
estB
Organism
Burkholderia gladioli (Pseudomonas marginata) (Phytomonas marginata)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Acts on short-chain (C4-C6) fatty acid esters and triglycerides, including tertiary alcohol esters. Activity on p-nitrophenyl esters is generally higher than on o-nitrophenyl esters. Lacks beta-lactamase activity; it hydrolyzes the ester bond of cephalosporin substrates but there is no opening of the beta-lactam ring observed.

Enzyme regulationi

Strongly inhibited by eserin, NaF, HgCl2, SDS and Triton X-100.

Kineticsi

Esterase, not beta-lactamase activity of the enzyme.

  1. KM=1.3 mM for cephalosporin C
  2. KM=1.0 mM for 7-amino cephalosporinic acid

Vmax=79 µmol/min/mg enzyme for cephalosporin C

Vmax=77 µmol/min/mg enzyme for 7-amino cephalosporinic acid

pH dependencei

Optimum pH is 7.0.

Temperature dependencei

Optimum temperature is 43 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751Acyl-ester intermediate

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Protein family/group databases

MEROPSiS12.950.

Names & Taxonomyi

Protein namesi
Recommended name:
Esterase EstB (EC:3.1.1.-)
Gene namesi
Name:estB
OrganismiBurkholderia gladioli (Pseudomonas marginata) (Phytomonas marginata)
Taxonomic identifieri28095 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia

Subcellular locationi

Cytoplasm Inferred 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Biotechnological usei

Directed evolutionary mutagenesis has created an enzyme with 40-fold higher stability in organic solvents. It contains the following changes: Leu-8, Ser-132, Arg-134, Cys-155, Gly-251, Val-311 and Lys-316 and could have applications in antibiotic synthesis (1 Publication).

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi75 – 751S → A: Complete loss of esterase activity. 1 Publication
Mutagenesisi149 – 1491S → A: Retains 30% esterase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Esterase EstBPRO_0000370699Add
BLAST

Structurei

Secondary structure

1
392
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 3116
Beta strandi36 – 449
Beta strandi47 – 5812
Turni59 – 624
Beta strandi70 – 723
Helixi74 – 763
Helixi77 – 9014
Helixi100 – 1023
Helixi121 – 1255
Helixi134 – 1374
Helixi142 – 1465
Beta strandi151 – 1533
Helixi159 – 16810
Helixi184 – 19613
Helixi200 – 2078
Helixi209 – 2124
Helixi224 – 2263
Beta strandi235 – 2373
Beta strandi245 – 2484
Beta strandi255 – 2595
Helixi261 – 2655
Turni273 – 2753
Helixi281 – 29313
Beta strandi295 – 2984
Helixi300 – 3078
Helixi313 – 3153
Beta strandi322 – 3243
Beta strandi326 – 3327
Helixi334 – 3374
Beta strandi346 – 3505
Turni351 – 3533
Beta strandi354 – 3596
Helixi360 – 3623
Beta strandi364 – 37310
Helixi375 – 3784
Helixi380 – 38910

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CI8X-ray2.00A/B1-392[»]
1CI9X-ray1.80A/B1-392[»]
ProteinModelPortaliQ9KX40.
SMRiQ9KX40. Positions 15-391.

Miscellaneous databases

EvolutionaryTraceiQ9KX40.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KX40-1 [UniParc]FASTAAdd to Basket

« Hide

MTAASLDPTA FSLDAASLAA RLDAVFDQAL RERRLVGAVA IVARHGEILY    50
RRAQGLADRE AGRPMREDTL FRLASVTKPI VALAVLRLVA RGELALDAPV 100
TRWLPEFRPR LADGSEPLVT IHHLLTHTSG LGYWLLEGAG SVYDRLGISD 150
GIDLRDFDLD ENLRRLASAP LSFAPGSGWQ YSLALDVLGA VVERATGQPL 200
AAAVDALVAQ PLGMRDCGFV SAEPERFAVP YHDGQPEPVR MRDGIEVPLP 250
EGHGAAVRFA PSRVFEPGAY PSGGAGMYGS ADDVLRALEA IRANPGFLPE 300
TLADAARRDQ AGVGAETRGP GWGFGYLSAV LDDPAAAGTP QHAGTLQWGG 350
VYGHSWFVDR ALGLSVLLLT NTAYEGMSGP LTIALRDAVY AR 392
Length:392
Mass (Da):41,707
Last modified:October 1, 2000 - v1
Checksum:i60EC4764C3C499B7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U33634 Genomic DNA. Translation: AAF59826.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U33634 Genomic DNA. Translation: AAF59826.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CI8 X-ray 2.00 A/B 1-392 [» ]
1CI9 X-ray 1.80 A/B 1-392 [» ]
ProteinModelPortali Q9KX40.
SMRi Q9KX40. Positions 15-391.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S12.950.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q9KX40.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
Pfami PF00144. Beta-lactamase. 1 hit.
[Graphical view ]
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A novel esterase from Burkholderia gladioli which shows high deacetylation activity on cephalosporins is related to beta-lactamases and DD-peptidases."
    Petersen E.I., Valinger G., Soelkner B., Stubenrauch G., Schwab H.
    J. Biotechnol. 89:11-25(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION AS AN ESTERASE, MUTAGENESIS OF SER-75 AND SER-149, ABSENCE OF BETA-LACTAMASE ACTIVITY, PROBABLE SUBCELLULAR LOCATION.
    Strain: ATCC 10248 / NCPPB 1891.
  2. "Stability and activity improvement of cephalosporin esterase EstB from Burkholderia gladioli by directed evolution and structural interpretation of muteins."
    Valinger G., Hermann M., Wagner U.G., Schwab H.
    J. Biotechnol. 129:98-108(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: RANDOM MUTAGENESIS TO CREATE MORE STABLE ORGANIC SOLVENT-RESISTANT ENZYME.
    Strain: ATCC 10248 / NCPPB 1891.
  3. "Inverting enantioselectivity of Burkholderia gladioli esterase EstB by directed and designed evolution."
    Ivancic M., Valinger G., Gruber K., Schwab H.
    J. Biotechnol. 129:109-122(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: RANDOM MUTAGENESIS TO ALTER ENANTIOSELECTIVITY.
    Strain: ATCC 10248 / NCPPB 1891.
  4. "EstB from Burkholderia gladioli: a novel esterase with a beta-lactamase fold reveals steric factors to discriminate between esterolytic and beta-lactam cleaving activity."
    Wagner U.G., Petersen E.I., Schwab H., Kratky C.
    Protein Sci. 11:467-478(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) WITH AND WITHOUT THE INHIBITOR DIISOPROPYL-FLUOROPHOSPHATE.
    Strain: ATCC 10248 / NCPPB 1891.

Entry informationi

Entry nameiESTB_BURGA
AccessioniPrimary (citable) accession number: Q9KX40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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