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Q9KWZ4

- TRMB_STAAC

UniProt

Q9KWZ4 - TRMB_STAAC

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, SACOL1798
Organism
Staphylococcus aureus (strain COL)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431S-adenosyl-L-methionine By similarity
Binding sitei68 – 681S-adenosyl-L-methionine By similarity
Binding sitei95 – 951S-adenosyl-L-methionine By similarity
Active sitei117 – 1171 By similarity
Binding sitei117 – 1171S-adenosyl-L-methionine By similarity
Binding sitei121 – 1211Substrate By similarity
Binding sitei153 – 1531Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSAUR93062:GCEP-1759-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:SACOL1798
    OrganismiStaphylococcus aureus (strain COL)
    Taxonomic identifieri93062 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000530: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 214214tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_0000171388Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi93062.SACOL1798.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9KWZ4.
    SMRiQ9KWZ4. Positions 9-210.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni190 – 1934Substrate binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000251689.
    KOiK03439.
    OMAiDNVMTEY.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9KWZ4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVRYKPWAK DYLKDHPELV DMDGQHAGKM TEWFDKTQPI HIEIGSGMGQ    50
    FITTLAAQNP HINYISMERE KSIVYKVLDK VKEMGLTNLK IICNDAIELN 100
    EYFKDGEVSR IYLNFSDPWP KNRHAKRRLT YHTFLALYQQ ILNDEGDLHF 150
    KTDNRGLFAY SLESMSQFGM YFTKINLNLH QEDDGSNILT EYEKKFSDKG 200
    SRIYRMEAKF HSQK 214
    Length:214
    Mass (Da):25,275
    Last modified:October 1, 2000 - v1
    Checksum:i789C314D41B2CC68
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y14816 Genomic DNA. Translation: CAB82477.1.
    CP000046 Genomic DNA. Translation: AAW38326.1.
    RefSeqiYP_186631.1. NC_002951.2.

    Genome annotation databases

    EnsemblBacteriaiAAW38326; AAW38326; SACOL1798.
    GeneIDi3236052.
    KEGGisac:SACOL1798.
    PATRICi19529799. VBIStaAur112458_1755.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y14816 Genomic DNA. Translation: CAB82477.1 .
    CP000046 Genomic DNA. Translation: AAW38326.1 .
    RefSeqi YP_186631.1. NC_002951.2.

    3D structure databases

    ProteinModelPortali Q9KWZ4.
    SMRi Q9KWZ4. Positions 9-210.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 93062.SACOL1798.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAW38326 ; AAW38326 ; SACOL1798 .
    GeneIDi 3236052.
    KEGGi sac:SACOL1798.
    PATRICi 19529799. VBIStaAur112458_1755.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000251689.
    KOi K03439.
    OMAi DNVMTEY.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci SAUR93062:GCEP-1759-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Antibiotic resistance as a stress response: complete sequencing of a large number of chromosomal loci in Staphylococcus aureus strain COL that impact on the expression of resistance to methicillin."
      de Lencastre H., Wu S.-W., Pinho M.G., Ludovice A.M., Filipe S., Gardete S., Sobral R., Gill S.R., Chung M., Tomasz A.
      Microb. Drug Resist. 5:163-175(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
      Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H.
      , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
      J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: COL.

    Entry informationi

    Entry nameiTRMB_STAAC
    AccessioniPrimary (citable) accession number: Q9KWZ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: October 1, 2000
    Last modified: June 11, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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