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Protein

DNA-directed RNA polymerase subunit beta

Gene

rpoB

Organism
Thermus aquaticus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit betaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit betaUniRule annotation
Alternative name(s):
RNA polymerase subunit betaUniRule annotation
Transcriptase subunit betaUniRule annotation
Gene namesi
Name:rpoBUniRule annotation
OrganismiThermus aquaticus
Taxonomic identifieri271 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11191119DNA-directed RNA polymerase subunit betaPRO_0000047983Add
BLAST

Proteomic databases

PRIDEiQ9KWU7.

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

Protein-protein interaction databases

STRINGi498848.TaqDRAFT_4913.

Structurei

Secondary structure

1
1119
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 54Combined sources
Helixi18 – 3013Combined sources
Turni36 – 383Combined sources
Beta strandi41 – 433Combined sources
Helixi44 – 518Combined sources
Beta strandi54 – 574Combined sources
Helixi59 – 624Combined sources
Beta strandi64 – 7310Combined sources
Helixi80 – 845Combined sources
Turni85 – 873Combined sources
Beta strandi91 – 10212Combined sources
Turni103 – 1053Combined sources
Beta strandi108 – 11912Combined sources
Beta strandi123 – 1253Combined sources
Beta strandi127 – 1293Combined sources
Beta strandi132 – 1354Combined sources
Beta strandi138 – 1425Combined sources
Beta strandi144 – 1463Combined sources
Beta strandi152 – 1554Combined sources
Beta strandi158 – 1636Combined sources
Beta strandi164 – 1696Combined sources
Beta strandi172 – 1754Combined sources
Beta strandi178 – 1803Combined sources
Beta strandi184 – 1863Combined sources
Beta strandi189 – 1913Combined sources
Helixi193 – 1997Combined sources
Helixi204 – 2118Combined sources
Beta strandi213 – 2164Combined sources
Helixi218 – 2225Combined sources
Helixi224 – 2296Combined sources
Helixi231 – 24212Combined sources
Beta strandi243 – 2453Combined sources
Helixi252 – 2554Combined sources
Turni256 – 2583Combined sources
Beta strandi259 – 2646Combined sources
Beta strandi269 – 2713Combined sources
Helixi274 – 2807Combined sources
Beta strandi281 – 2833Combined sources
Beta strandi287 – 2915Combined sources
Turni294 – 2963Combined sources
Helixi303 – 3119Combined sources
Turni312 – 3154Combined sources
Beta strandi316 – 3183Combined sources
Beta strandi330 – 3345Combined sources
Helixi336 – 35116Combined sources
Turni352 – 3543Combined sources
Helixi355 – 3584Combined sources
Beta strandi360 – 3623Combined sources
Turni364 – 3663Combined sources
Helixi369 – 3724Combined sources
Helixi376 – 38510Combined sources
Beta strandi390 – 3945Combined sources
Helixi400 – 4078Combined sources
Beta strandi409 – 4124Combined sources
Beta strandi414 – 4185Combined sources
Turni426 – 4283Combined sources
Turni435 – 4373Combined sources
Beta strandi445 – 4484Combined sources
Turni449 – 4513Combined sources
Beta strandi452 – 4554Combined sources
Beta strandi456 – 4583Combined sources
Beta strandi467 – 4693Combined sources
Beta strandi471 – 4755Combined sources
Beta strandi478 – 4836Combined sources
Turni490 – 4934Combined sources
Beta strandi499 – 5013Combined sources
Beta strandi511 – 5177Combined sources
Beta strandi520 – 5245Combined sources
Helixi526 – 5283Combined sources
Beta strandi531 – 5344Combined sources
Helixi536 – 5394Combined sources
Helixi544 – 5463Combined sources
Helixi550 – 5523Combined sources
Helixi555 – 5628Combined sources
Helixi564 – 5674Combined sources
Beta strandi578 – 5803Combined sources
Helixi583 – 5908Combined sources
Beta strandi594 – 5963Combined sources
Beta strandi598 – 6003Combined sources
Beta strandi602 – 6065Combined sources
Beta strandi608 – 6147Combined sources
Beta strandi616 – 6183Combined sources
Beta strandi621 – 6244Combined sources
Beta strandi650 – 6523Combined sources
Beta strandi654 – 6574Combined sources
Beta strandi659 – 6624Combined sources
Beta strandi668 – 67710Combined sources
Turni680 – 6834Combined sources
Beta strandi688 – 6914Combined sources
Helixi693 – 6964Combined sources
Beta strandi703 – 71311Combined sources
Turni727 – 7304Combined sources
Turni731 – 7333Combined sources
Helixi734 – 7363Combined sources
Beta strandi741 – 7433Combined sources
Beta strandi755 – 7573Combined sources
Helixi771 – 7777Combined sources
Beta strandi798 – 8069Combined sources
Beta strandi808 – 8103Combined sources
Beta strandi819 – 83012Combined sources
Beta strandi838 – 8403Combined sources
Beta strandi842 – 8443Combined sources
Beta strandi847 – 8537Combined sources
Beta strandi868 – 8714Combined sources
Helixi873 – 8753Combined sources
Turni876 – 8783Combined sources
Helixi883 – 89715Combined sources
Beta strandi899 – 9024Combined sources
Turni905 – 9073Combined sources
Helixi911 – 93222Combined sources
Helixi938 – 94912Combined sources
Beta strandi955 – 9573Combined sources
Helixi959 – 96810Combined sources
Beta strandi977 – 9826Combined sources
Beta strandi987 – 99610Combined sources
Turni998 – 10003Combined sources
Turni1001 – 10033Combined sources
Beta strandi1008 – 10103Combined sources
Turni1015 – 10173Combined sources
Beta strandi1024 – 10263Combined sources
Helixi1034 – 10429Combined sources
Helixi1046 – 10538Combined sources
Turni1054 – 10585Combined sources
Helixi1060 – 107011Combined sources
Turni1071 – 10733Combined sources
Helixi1083 – 109311Combined sources
Beta strandi1100 – 11023Combined sources
Beta strandi1106 – 11083Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HQMX-ray3.30C1-1119[»]
1I6VX-ray3.30C1-1119[»]
1L9UX-ray4.00C/L1-1119[»]
1L9ZX-ray6.50C1-1119[»]
1YNJX-ray3.20C1-1119[»]
1YNNX-ray3.30C1-1119[»]
2GHOX-ray5.00C1-1119[»]
3MLQX-ray2.91A/B/C/D17-139[»]
A/B/C/D334-395[»]
4XAXX-ray2.40A17-139[»]
A334-392[»]
4XLNX-ray4.00C/I1-1119[»]
4XLPX-ray4.00C/I1-1119[»]
4XLQX-ray4.60C/I1-1119[»]
4XLRX-ray4.30C/I1-1119[»]
4XLSX-ray4.01C/I1-1119[»]
ProteinModelPortaliQ9KWU7.
SMRiQ9KWU7. Positions 1-1114.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KWU7.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QIH. Bacteria.
COG0085. LUCA.

Family and domain databases

Gene3Di2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
HAMAPiMF_01321. RNApol_bact_RpoB.
InterProiIPR010243. DNA-dir_RNA_pol_bsu.
IPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 2 hits.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02013. rpoB. 1 hit.
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KWU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIKRFGRIR EVIPLPPLTE IQVESYKKAL QADVPPEKRE NVGIQAAFKE
60 70 80 90 100
TFPIEEGDKG KGGLVLDFLE YRIGDPPFSQ DECREKDLTY QAPLYARLQL
110 120 130 140 150
IHKDTGLIKE DEVFLGHLPL MTEDGSFIIN GADRVIVSQI HRSPGVYFTP
160 170 180 190 200
DPARPGRYIA SIIPLPKRGP WIDLEVEASG VVTMKVNKRK FPLVLLLRVL
210 220 230 240 250
GYDQETLVRE LSAYGDLVQG LLDEAVLAMR PEEAMVRLFT LLRPGDPPKK
260 270 280 290 300
DKALAYLFGL LADPKRYDLG EAGRYKAEEK LGVGLSGRTL VRFEDGEFKD
310 320 330 340 350
EVFLPTLRYL FALTAGVPGH EVDDIDHLGN RRIRTVGELM ADQFRVGLAR
360 370 380 390 400
LARGVRERMV MGSPDTLTPA KLVNSRPLEA ALREFFSRSQ LSQFKDETNP
410 420 430 440 450
LSSLRHKRRI SALGPGGLTR ERAGFDVRDV HRTHYGRICP VETPEGANIG
460 470 480 490 500
LITSLAAYAR VDALGFIRTP YRRVKNGVVT EEVVYMTASE EDRYTIAQAN
510 520 530 540 550
TPLEGDRIAT DRVVARRRGE PVIVAPEEVE FMDVSPKQVF SLNTNLIPFL
560 570 580 590 600
EHDDANRALM GSNMQTQAVP LIRAQAPVVM TGLEERVVRD SLAALYAEED
610 620 630 640 650
GEVVKVDGTR IAVRYEDGRL VEHPLRRYAR SNQGTAFDQR PRVRVGQRVK
660 670 680 690 700
KGDLLADGPA SEEGFLALGQ NVLVAIMPFD GYNFEDAIVI SEELLKRDFY
710 720 730 740 750
TSIHIERYEI EARDTKLGPE RITRDIPHLS EAALRDLDEE GIVRIGAEVK
760 770 780 790 800
PGDILVGRTS FKGEQEPSPE ERLLRSIFGE KARDVKDTSL RVPPGEGGIV
810 820 830 840 850
VGRLRLRRGD PGVELKPGVR EVVRVFVAQK RKLQVGDKLA NRHGNKGVVA
860 870 880 890 900
KILPVEDMPH LPDGTPVDVI LNPLGVPSRM NLGQILETHL GLAGYFLGQR
910 920 930 940 950
YISPVFDGAT EPEIKELLAE AFNLYFGKRQ GEGFGVDKRE KEVLARAEKL
960 970 980 990 1000
GLVSPGKSPE EQLKELFDLG KVVLYDGRTG EPFEGPIVVG QMFIMKLYHM
1010 1020 1030 1040 1050
VEDKMHARST GPYSLITQQP LGGKAQFGGQ RFGEMEVWAL EAYGAAHTLQ
1060 1070 1080 1090 1100
EMLTIKSDDI EGRNAAYQAI IKGEDVPEPS VPESFRVLVK ELQALALDVQ
1110
TLDEKDNPVD IFEGLASKR
Length:1,119
Mass (Da):124,758
Last modified:October 1, 2000 - v1
Checksum:i2CEF66FA79C77F33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y19223 Genomic DNA. Translation: CAB65465.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y19223 Genomic DNA. Translation: CAB65465.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HQMX-ray3.30C1-1119[»]
1I6VX-ray3.30C1-1119[»]
1L9UX-ray4.00C/L1-1119[»]
1L9ZX-ray6.50C1-1119[»]
1YNJX-ray3.20C1-1119[»]
1YNNX-ray3.30C1-1119[»]
2GHOX-ray5.00C1-1119[»]
3MLQX-ray2.91A/B/C/D17-139[»]
A/B/C/D334-395[»]
4XAXX-ray2.40A17-139[»]
A334-392[»]
4XLNX-ray4.00C/I1-1119[»]
4XLPX-ray4.00C/I1-1119[»]
4XLQX-ray4.60C/I1-1119[»]
4XLRX-ray4.30C/I1-1119[»]
4XLSX-ray4.01C/I1-1119[»]
ProteinModelPortaliQ9KWU7.
SMRiQ9KWU7. Positions 1-1114.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498848.TaqDRAFT_4913.

Proteomic databases

PRIDEiQ9KWU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4107QIH. Bacteria.
COG0085. LUCA.

Miscellaneous databases

EvolutionaryTraceiQ9KWU7.

Family and domain databases

Gene3Di2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
HAMAPiMF_01321. RNApol_bact_RpoB.
InterProiIPR010243. DNA-dir_RNA_pol_bsu.
IPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 2 hits.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02013. rpoB. 1 hit.
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 A resolution."
    Zhang G., Campbell E.A., Minakhin L., Richter C., Severinov K., Darst S.A.
    Cell 98:811-824(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS).

Entry informationi

Entry nameiRPOB_THEAQ
AccessioniPrimary (citable) accession number: Q9KWU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2000
Last modified: December 9, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.