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Protein

DNA-directed RNA polymerase subunit beta

Gene

rpoB

Organism
Thermus aquaticus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit betaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit betaUniRule annotation
Alternative name(s):
RNA polymerase subunit betaUniRule annotation
Transcriptase subunit betaUniRule annotation
Gene namesi
Name:rpoBUniRule annotation
OrganismiThermus aquaticus
Taxonomic identifieri271 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000479831 – 1119DNA-directed RNA polymerase subunit betaAdd BLAST1119

Proteomic databases

PRIDEiQ9KWU7.

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

Protein-protein interaction databases

STRINGi498848.TaqDRAFT_4913.

Structurei

Secondary structure

11119
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi18 – 30Combined sources13
Turni36 – 38Combined sources3
Beta strandi41 – 43Combined sources3
Helixi44 – 51Combined sources8
Beta strandi54 – 57Combined sources4
Helixi59 – 62Combined sources4
Beta strandi64 – 73Combined sources10
Helixi80 – 84Combined sources5
Turni85 – 87Combined sources3
Beta strandi91 – 102Combined sources12
Turni103 – 105Combined sources3
Beta strandi108 – 119Combined sources12
Beta strandi123 – 125Combined sources3
Beta strandi127 – 129Combined sources3
Beta strandi132 – 135Combined sources4
Beta strandi138 – 142Combined sources5
Beta strandi144 – 146Combined sources3
Beta strandi152 – 155Combined sources4
Beta strandi158 – 163Combined sources6
Beta strandi164 – 169Combined sources6
Beta strandi172 – 175Combined sources4
Beta strandi178 – 180Combined sources3
Beta strandi184 – 186Combined sources3
Beta strandi189 – 191Combined sources3
Helixi193 – 199Combined sources7
Helixi204 – 211Combined sources8
Beta strandi213 – 216Combined sources4
Helixi218 – 222Combined sources5
Helixi224 – 229Combined sources6
Helixi231 – 242Combined sources12
Beta strandi243 – 245Combined sources3
Helixi252 – 255Combined sources4
Turni256 – 258Combined sources3
Beta strandi259 – 264Combined sources6
Beta strandi269 – 271Combined sources3
Helixi274 – 280Combined sources7
Beta strandi281 – 283Combined sources3
Beta strandi287 – 291Combined sources5
Turni294 – 296Combined sources3
Helixi303 – 311Combined sources9
Turni312 – 315Combined sources4
Beta strandi316 – 318Combined sources3
Beta strandi330 – 334Combined sources5
Helixi336 – 351Combined sources16
Turni352 – 354Combined sources3
Helixi355 – 358Combined sources4
Beta strandi360 – 362Combined sources3
Turni364 – 366Combined sources3
Helixi369 – 372Combined sources4
Helixi376 – 385Combined sources10
Beta strandi390 – 394Combined sources5
Helixi400 – 407Combined sources8
Beta strandi409 – 412Combined sources4
Beta strandi414 – 418Combined sources5
Turni426 – 428Combined sources3
Turni435 – 437Combined sources3
Beta strandi445 – 448Combined sources4
Turni449 – 451Combined sources3
Beta strandi452 – 455Combined sources4
Beta strandi456 – 458Combined sources3
Beta strandi467 – 469Combined sources3
Beta strandi471 – 475Combined sources5
Beta strandi478 – 483Combined sources6
Turni490 – 493Combined sources4
Beta strandi499 – 501Combined sources3
Beta strandi511 – 517Combined sources7
Beta strandi520 – 524Combined sources5
Helixi526 – 528Combined sources3
Beta strandi531 – 534Combined sources4
Helixi536 – 539Combined sources4
Helixi544 – 546Combined sources3
Helixi550 – 552Combined sources3
Helixi555 – 562Combined sources8
Helixi564 – 567Combined sources4
Beta strandi578 – 580Combined sources3
Helixi583 – 590Combined sources8
Beta strandi594 – 596Combined sources3
Beta strandi598 – 600Combined sources3
Beta strandi602 – 606Combined sources5
Beta strandi608 – 614Combined sources7
Beta strandi616 – 618Combined sources3
Beta strandi621 – 624Combined sources4
Beta strandi650 – 652Combined sources3
Beta strandi654 – 657Combined sources4
Beta strandi659 – 662Combined sources4
Beta strandi668 – 677Combined sources10
Turni680 – 683Combined sources4
Beta strandi688 – 691Combined sources4
Helixi693 – 696Combined sources4
Beta strandi703 – 713Combined sources11
Turni727 – 730Combined sources4
Turni731 – 733Combined sources3
Helixi734 – 736Combined sources3
Beta strandi741 – 743Combined sources3
Beta strandi755 – 757Combined sources3
Helixi771 – 777Combined sources7
Beta strandi798 – 806Combined sources9
Beta strandi808 – 810Combined sources3
Beta strandi819 – 830Combined sources12
Beta strandi838 – 840Combined sources3
Beta strandi842 – 844Combined sources3
Beta strandi847 – 853Combined sources7
Beta strandi868 – 871Combined sources4
Helixi873 – 875Combined sources3
Turni876 – 878Combined sources3
Helixi883 – 897Combined sources15
Beta strandi899 – 902Combined sources4
Turni905 – 907Combined sources3
Helixi911 – 932Combined sources22
Helixi938 – 949Combined sources12
Beta strandi955 – 957Combined sources3
Helixi959 – 968Combined sources10
Beta strandi977 – 982Combined sources6
Beta strandi987 – 996Combined sources10
Turni998 – 1000Combined sources3
Turni1001 – 1003Combined sources3
Beta strandi1008 – 1010Combined sources3
Turni1015 – 1017Combined sources3
Beta strandi1024 – 1026Combined sources3
Helixi1034 – 1042Combined sources9
Helixi1046 – 1053Combined sources8
Turni1054 – 1058Combined sources5
Helixi1060 – 1070Combined sources11
Turni1071 – 1073Combined sources3
Helixi1083 – 1093Combined sources11
Beta strandi1100 – 1102Combined sources3
Beta strandi1106 – 1108Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HQMX-ray3.30C1-1119[»]
1I6VX-ray3.30C1-1119[»]
1L9UX-ray4.00C/L1-1119[»]
1L9ZX-ray6.50C1-1119[»]
1YNJX-ray3.20C1-1119[»]
1YNNX-ray3.30C1-1119[»]
2GHOX-ray5.00C1-1119[»]
3MLQX-ray2.91A/B/C/D17-139[»]
A/B/C/D334-395[»]
4XAXX-ray2.40A17-139[»]
A334-392[»]
4XLNX-ray4.00C/I1-1119[»]
4XLPX-ray4.00C/I1-1119[»]
4XLQX-ray4.60C/I1-1119[»]
4XLRX-ray4.30C/I1-1119[»]
4XLSX-ray4.01C/I1-1119[»]
ProteinModelPortaliQ9KWU7.
SMRiQ9KWU7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KWU7.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QIH. Bacteria.
COG0085. LUCA.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
HAMAPiMF_01321. RNApol_bact_RpoB. 1 hit.
InterProiIPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR010243. RNA_pol_bsu_bac.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 2 hits.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02013. rpoB. 1 hit.
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KWU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIKRFGRIR EVIPLPPLTE IQVESYKKAL QADVPPEKRE NVGIQAAFKE
60 70 80 90 100
TFPIEEGDKG KGGLVLDFLE YRIGDPPFSQ DECREKDLTY QAPLYARLQL
110 120 130 140 150
IHKDTGLIKE DEVFLGHLPL MTEDGSFIIN GADRVIVSQI HRSPGVYFTP
160 170 180 190 200
DPARPGRYIA SIIPLPKRGP WIDLEVEASG VVTMKVNKRK FPLVLLLRVL
210 220 230 240 250
GYDQETLVRE LSAYGDLVQG LLDEAVLAMR PEEAMVRLFT LLRPGDPPKK
260 270 280 290 300
DKALAYLFGL LADPKRYDLG EAGRYKAEEK LGVGLSGRTL VRFEDGEFKD
310 320 330 340 350
EVFLPTLRYL FALTAGVPGH EVDDIDHLGN RRIRTVGELM ADQFRVGLAR
360 370 380 390 400
LARGVRERMV MGSPDTLTPA KLVNSRPLEA ALREFFSRSQ LSQFKDETNP
410 420 430 440 450
LSSLRHKRRI SALGPGGLTR ERAGFDVRDV HRTHYGRICP VETPEGANIG
460 470 480 490 500
LITSLAAYAR VDALGFIRTP YRRVKNGVVT EEVVYMTASE EDRYTIAQAN
510 520 530 540 550
TPLEGDRIAT DRVVARRRGE PVIVAPEEVE FMDVSPKQVF SLNTNLIPFL
560 570 580 590 600
EHDDANRALM GSNMQTQAVP LIRAQAPVVM TGLEERVVRD SLAALYAEED
610 620 630 640 650
GEVVKVDGTR IAVRYEDGRL VEHPLRRYAR SNQGTAFDQR PRVRVGQRVK
660 670 680 690 700
KGDLLADGPA SEEGFLALGQ NVLVAIMPFD GYNFEDAIVI SEELLKRDFY
710 720 730 740 750
TSIHIERYEI EARDTKLGPE RITRDIPHLS EAALRDLDEE GIVRIGAEVK
760 770 780 790 800
PGDILVGRTS FKGEQEPSPE ERLLRSIFGE KARDVKDTSL RVPPGEGGIV
810 820 830 840 850
VGRLRLRRGD PGVELKPGVR EVVRVFVAQK RKLQVGDKLA NRHGNKGVVA
860 870 880 890 900
KILPVEDMPH LPDGTPVDVI LNPLGVPSRM NLGQILETHL GLAGYFLGQR
910 920 930 940 950
YISPVFDGAT EPEIKELLAE AFNLYFGKRQ GEGFGVDKRE KEVLARAEKL
960 970 980 990 1000
GLVSPGKSPE EQLKELFDLG KVVLYDGRTG EPFEGPIVVG QMFIMKLYHM
1010 1020 1030 1040 1050
VEDKMHARST GPYSLITQQP LGGKAQFGGQ RFGEMEVWAL EAYGAAHTLQ
1060 1070 1080 1090 1100
EMLTIKSDDI EGRNAAYQAI IKGEDVPEPS VPESFRVLVK ELQALALDVQ
1110
TLDEKDNPVD IFEGLASKR
Length:1,119
Mass (Da):124,758
Last modified:October 1, 2000 - v1
Checksum:i2CEF66FA79C77F33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y19223 Genomic DNA. Translation: CAB65465.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y19223 Genomic DNA. Translation: CAB65465.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HQMX-ray3.30C1-1119[»]
1I6VX-ray3.30C1-1119[»]
1L9UX-ray4.00C/L1-1119[»]
1L9ZX-ray6.50C1-1119[»]
1YNJX-ray3.20C1-1119[»]
1YNNX-ray3.30C1-1119[»]
2GHOX-ray5.00C1-1119[»]
3MLQX-ray2.91A/B/C/D17-139[»]
A/B/C/D334-395[»]
4XAXX-ray2.40A17-139[»]
A334-392[»]
4XLNX-ray4.00C/I1-1119[»]
4XLPX-ray4.00C/I1-1119[»]
4XLQX-ray4.60C/I1-1119[»]
4XLRX-ray4.30C/I1-1119[»]
4XLSX-ray4.01C/I1-1119[»]
ProteinModelPortaliQ9KWU7.
SMRiQ9KWU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498848.TaqDRAFT_4913.

Proteomic databases

PRIDEiQ9KWU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4107QIH. Bacteria.
COG0085. LUCA.

Miscellaneous databases

EvolutionaryTraceiQ9KWU7.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
HAMAPiMF_01321. RNApol_bact_RpoB. 1 hit.
InterProiIPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR010243. RNA_pol_bsu_bac.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 2 hits.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02013. rpoB. 1 hit.
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPOB_THEAQ
AccessioniPrimary (citable) accession number: Q9KWU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.