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Q9KWU1

- Q9KWU1_SPHSX

UniProt

Q9KWU1 - Q9KWU1_SPHSX

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Protein
Submitted name:

Alginate lyase

Gene
aly
Organism
Sphingomonas sp.
Status
Unreviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei239 – 2391Transition state stabilizerImported
Sitei287 – 2871Important for catalytic activityImported
Active sitei294 – 2941Proton donor/acceptorImported

GO - Molecular functioni

  1. poly(beta-D-mannuronate) lyase activity Source: InterPro

GO - Biological processi

  1. alginic acid catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LyaseImported

Protein family/group databases

CAZyiPL5. Polysaccharide Lyase Family 5.
PL7. Polysaccharide Lyase Family 7.

Names & Taxonomyi

Protein namesi
Submitted name:
Alginate lyaseImported
Gene namesi
Name:alyImported
OrganismiSphingomonas sp.Imported
Taxonomic identifieri28214 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: InterPro
Complete GO annotation...

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HV6X-ray2.00A54-402[»]
1QAZX-ray1.78A54-402[»]
4E1YX-ray2.10A/B54-404[»]
4F10X-ray2.20A/B54-404[»]
4F13X-ray2.21A/B54-404[»]
ProteinModelPortaliQ9KWU1.
SMRiQ9KWU1. Positions 53-402, 415-641.

Miscellaneous databases

EvolutionaryTraceiQ9KWU1.

Family & Domainsi

Family and domain databases

Gene3Di1.50.10.110. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR014895. Alginate_lyase_2.
IPR008397. Alginate_lyase_dom.
IPR008929. Chondroitin_lyas.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
[Graphical view]
PfamiPF05426. Alginate_lyase. 1 hit.
PF08787. Alginate_lyase2. 1 hit.
[Graphical view]
SUPFAMiSSF48230. SSF48230. 1 hit.
SSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KWU1-1 [UniParc]FASTAAdd to Basket

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MPLACLATTR VGAAREKSGD SSMFDIPFPG HGRRLAVAAL AFAGCAFAGS    50
LQAHPFDQAV VKDPTASYVD VKARRTFLQS GQLDDRLKAA LPKEYDCTTE 100
ATPNPQQGEM VIPRRYLSGN HGPVNPDYEP VVTLYRDFEK ISATLGNLYV 150
ATGKPVYATC LLNMLDKWAK ADALLNYDPK SQSWYQVEWS AATAAFALST 200
MMAEPNVDTA QRERVVKWLN RVARHQTSFP GGDTSCCNNH SYWRGQEATI 250
IGVISKDDEL FRWGLGRYVQ AMGLINEDGS FVHEMTRHEQ SLHYQNYAML 300
PLTMIAETAS RQGIDLYAYK ENGRDIHSAR KFVFAAVKNP DLIKKYASEP 350
QDTRAFKPGR GDLNWIEYQR ARFGFADELG FMTVPIFDPR TGGSGTLLAY 400
KPQGAAAQAP VSAPAAAHSS IDLSKWKLQI PVDPIDVATR DLLKGYQDKY 450
FYVDKDGSLA FWCPASGFKT TANTKYPRSE LREMLDPDNH AVNWGWQGTH 500
EMNLRGAVMH VSPSGKTIVM QIHAVMPDGS NAPPLVKGQF YKNTLDFLVK 550
NSAAGGKDTH YVFEGIELGK PYDAQIKVVD GVLSMTVNGQ TKTVDFVAKD 600
AGWKDLKFYF KAGNYLQDRQ ADGSDTSALV KLYKLDVKHS S 641
Length:641
Mass (Da):71,003
Last modified:October 1, 2000 - v1
Checksum:i6C3C655070B7AD01
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB011415 Genomic DNA. Translation: BAB03312.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB011415 Genomic DNA. Translation: BAB03312.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HV6 X-ray 2.00 A 54-402 [» ]
1QAZ X-ray 1.78 A 54-402 [» ]
4E1Y X-ray 2.10 A/B 54-404 [» ]
4F10 X-ray 2.20 A/B 54-404 [» ]
4F13 X-ray 2.21 A/B 54-404 [» ]
ProteinModelPortali Q9KWU1.
SMRi Q9KWU1. Positions 53-402, 415-641.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi PL5. Polysaccharide Lyase Family 5.
PL7. Polysaccharide Lyase Family 7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q9KWU1.

Family and domain databases

Gene3Di 1.50.10.110. 1 hit.
2.60.120.200. 1 hit.
InterProi IPR014895. Alginate_lyase_2.
IPR008397. Alginate_lyase_dom.
IPR008929. Chondroitin_lyas.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
[Graphical view ]
Pfami PF05426. Alginate_lyase. 1 hit.
PF08787. Alginate_lyase2. 1 hit.
[Graphical view ]
SUPFAMi SSF48230. SSF48230. 1 hit.
SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Crystal structure of alginate lyase A1-III from Sphingomonas species A1 at 1.78 A resolution."
    Yoon H.J., Mikami B., Hashimoto W., Murata K.
    J. Mol. Biol. 290:505-514(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 54-402, ACTIVE SITE.
  2. "A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules."
    Momma K., Okamoto M., Mishima Y., Mori S., Hashimoto W., Murata K.
    J. Bacteriol. 182:3998-4004(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: A1Imported.
  3. "Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution."
    Yoon H.J., Hashimoto W., Miyake O., Murata K., Mikami B.
    J. Mol. Biol. 307:9-16(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 54-402.
  4. "Induced-fit motion of a lid loop involved in catalysis in alginate lyase A1-III."
    Mikami B., Ban M., Suzuki S., Yoon H.J., Miyake O., Yamasaki M., Ogura K., Maruyama Y., Hashimoto W., Murata K.
    Acta Crystallogr. D 68:1207-1216(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 54-404.

Entry informationi

Entry nameiQ9KWU1_SPHSX
AccessioniPrimary (citable) accession number: Q9KWU1
Entry historyi
Integrated into UniProtKB/TrEMBL: October 1, 2000
Last sequence update: October 1, 2000
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3

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