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Q9KWU1

- Q9KWU1_SPHSX

UniProt

Q9KWU1 - Q9KWU1_SPHSX

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Protein
Submitted name:

Alginate lyase

Gene

aly

Organism
Sphingomonas sp.
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei239 – 2391Transition state stabilizerImported
Sitei287 – 2871Important for catalytic activityImported
Active sitei294 – 2941Proton donor/acceptorImported

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LyaseImported

Protein family/group databases

CAZyiPL5. Polysaccharide Lyase Family 5.
PL7. Polysaccharide Lyase Family 7.

Names & Taxonomyi

Protein namesi
Submitted name:
Alginate lyaseImported
Gene namesi
Name:alyImported
OrganismiSphingomonas sp.Imported
Taxonomic identifieri28214 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: InterPro
Complete GO annotation...

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HV6X-ray2.00A54-402[»]
1QAZX-ray1.78A54-402[»]
4E1YX-ray2.10A/B54-404[»]
4F10X-ray2.20A/B54-404[»]
4F13X-ray2.21A/B54-404[»]
ProteinModelPortaliQ9KWU1.
SMRiQ9KWU1. Positions 53-402, 415-641.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KWU1.

Family & Domainsi

Family and domain databases

Gene3Di1.50.10.110. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR014895. Alginate_lyase_2.
IPR008397. Alginate_lyase_dom.
IPR008929. Chondroitin_lyas.
IPR013320. ConA-like_dom.
[Graphical view]
PfamiPF05426. Alginate_lyase. 1 hit.
PF08787. Alginate_lyase2. 1 hit.
[Graphical view]
SUPFAMiSSF48230. SSF48230. 1 hit.
SSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KWU1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLACLATTR VGAAREKSGD SSMFDIPFPG HGRRLAVAAL AFAGCAFAGS
60 70 80 90 100
LQAHPFDQAV VKDPTASYVD VKARRTFLQS GQLDDRLKAA LPKEYDCTTE
110 120 130 140 150
ATPNPQQGEM VIPRRYLSGN HGPVNPDYEP VVTLYRDFEK ISATLGNLYV
160 170 180 190 200
ATGKPVYATC LLNMLDKWAK ADALLNYDPK SQSWYQVEWS AATAAFALST
210 220 230 240 250
MMAEPNVDTA QRERVVKWLN RVARHQTSFP GGDTSCCNNH SYWRGQEATI
260 270 280 290 300
IGVISKDDEL FRWGLGRYVQ AMGLINEDGS FVHEMTRHEQ SLHYQNYAML
310 320 330 340 350
PLTMIAETAS RQGIDLYAYK ENGRDIHSAR KFVFAAVKNP DLIKKYASEP
360 370 380 390 400
QDTRAFKPGR GDLNWIEYQR ARFGFADELG FMTVPIFDPR TGGSGTLLAY
410 420 430 440 450
KPQGAAAQAP VSAPAAAHSS IDLSKWKLQI PVDPIDVATR DLLKGYQDKY
460 470 480 490 500
FYVDKDGSLA FWCPASGFKT TANTKYPRSE LREMLDPDNH AVNWGWQGTH
510 520 530 540 550
EMNLRGAVMH VSPSGKTIVM QIHAVMPDGS NAPPLVKGQF YKNTLDFLVK
560 570 580 590 600
NSAAGGKDTH YVFEGIELGK PYDAQIKVVD GVLSMTVNGQ TKTVDFVAKD
610 620 630 640
AGWKDLKFYF KAGNYLQDRQ ADGSDTSALV KLYKLDVKHS S
Length:641
Mass (Da):71,003
Last modified:October 1, 2000 - v1
Checksum:i6C3C655070B7AD01
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB011415 Genomic DNA. Translation: BAB03312.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB011415 Genomic DNA. Translation: BAB03312.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HV6 X-ray 2.00 A 54-402 [» ]
1QAZ X-ray 1.78 A 54-402 [» ]
4E1Y X-ray 2.10 A/B 54-404 [» ]
4F10 X-ray 2.20 A/B 54-404 [» ]
4F13 X-ray 2.21 A/B 54-404 [» ]
ProteinModelPortali Q9KWU1.
SMRi Q9KWU1. Positions 53-402, 415-641.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi PL5. Polysaccharide Lyase Family 5.
PL7. Polysaccharide Lyase Family 7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q9KWU1.

Family and domain databases

Gene3Di 1.50.10.110. 1 hit.
2.60.120.200. 1 hit.
InterProi IPR014895. Alginate_lyase_2.
IPR008397. Alginate_lyase_dom.
IPR008929. Chondroitin_lyas.
IPR013320. ConA-like_dom.
[Graphical view ]
Pfami PF05426. Alginate_lyase. 1 hit.
PF08787. Alginate_lyase2. 1 hit.
[Graphical view ]
SUPFAMi SSF48230. SSF48230. 1 hit.
SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Crystal structure of alginate lyase A1-III from Sphingomonas species A1 at 1.78 A resolution."
    Yoon H.J., Mikami B., Hashimoto W., Murata K.
    J. Mol. Biol. 290:505-514(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 54-402, ACTIVE SITE.
  2. "A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules."
    Momma K., Okamoto M., Mishima Y., Mori S., Hashimoto W., Murata K.
    J. Bacteriol. 182:3998-4004(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: A1Imported.
  3. "Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution."
    Yoon H.J., Hashimoto W., Miyake O., Murata K., Mikami B.
    J. Mol. Biol. 307:9-16(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 54-402.
  4. "Induced-fit motion of a lid loop involved in catalysis in alginate lyase A1-III."
    Mikami B., Ban M., Suzuki S., Yoon H.J., Miyake O., Yamasaki M., Ogura K., Maruyama Y., Hashimoto W., Murata K.
    Acta Crystallogr. D 68:1207-1216(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 54-404.

Entry informationi

Entry nameiQ9KWU1_SPHSX
AccessioniPrimary (citable) accession number: Q9KWU1
Entry historyi
Integrated into UniProtKB/TrEMBL: October 1, 2000
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3