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Q9KWU1

- Q9KWU1_SPHSX

UniProt

Q9KWU1 - Q9KWU1_SPHSX

Protein
Submitted name:

Alginate lyase

Gene

aly

Organism
Sphingomonas sp.
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei239 – 2391Transition state stabilizerImported
    Sitei287 – 2871Important for catalytic activityImported
    Active sitei294 – 2941Proton donor/acceptorImported

    GO - Molecular functioni

    1. poly(beta-D-mannuronate) lyase activity Source: InterPro

    GO - Biological processi

    1. alginic acid catabolic process Source: InterPro

    Keywords - Molecular functioni

    LyaseImported

    Protein family/group databases

    CAZyiPL5. Polysaccharide Lyase Family 5.
    PL7. Polysaccharide Lyase Family 7.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Alginate lyaseImported
    Gene namesi
    Name:alyImported
    OrganismiSphingomonas sp.Imported
    Taxonomic identifieri28214 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

    Subcellular locationi

    GO - Cellular componenti

    1. periplasmic space Source: InterPro

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HV6X-ray2.00A54-402[»]
    1QAZX-ray1.78A54-402[»]
    4E1YX-ray2.10A/B54-404[»]
    4F10X-ray2.20A/B54-404[»]
    4F13X-ray2.21A/B54-404[»]
    ProteinModelPortaliQ9KWU1.
    SMRiQ9KWU1. Positions 53-402, 415-641.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9KWU1.

    Family & Domainsi

    Family and domain databases

    Gene3Di1.50.10.110. 1 hit.
    2.60.120.200. 1 hit.
    InterProiIPR014895. Alginate_lyase_2.
    IPR008397. Alginate_lyase_dom.
    IPR008929. Chondroitin_lyas.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view]
    PfamiPF05426. Alginate_lyase. 1 hit.
    PF08787. Alginate_lyase2. 1 hit.
    [Graphical view]
    SUPFAMiSSF48230. SSF48230. 1 hit.
    SSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9KWU1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLACLATTR VGAAREKSGD SSMFDIPFPG HGRRLAVAAL AFAGCAFAGS    50
    LQAHPFDQAV VKDPTASYVD VKARRTFLQS GQLDDRLKAA LPKEYDCTTE 100
    ATPNPQQGEM VIPRRYLSGN HGPVNPDYEP VVTLYRDFEK ISATLGNLYV 150
    ATGKPVYATC LLNMLDKWAK ADALLNYDPK SQSWYQVEWS AATAAFALST 200
    MMAEPNVDTA QRERVVKWLN RVARHQTSFP GGDTSCCNNH SYWRGQEATI 250
    IGVISKDDEL FRWGLGRYVQ AMGLINEDGS FVHEMTRHEQ SLHYQNYAML 300
    PLTMIAETAS RQGIDLYAYK ENGRDIHSAR KFVFAAVKNP DLIKKYASEP 350
    QDTRAFKPGR GDLNWIEYQR ARFGFADELG FMTVPIFDPR TGGSGTLLAY 400
    KPQGAAAQAP VSAPAAAHSS IDLSKWKLQI PVDPIDVATR DLLKGYQDKY 450
    FYVDKDGSLA FWCPASGFKT TANTKYPRSE LREMLDPDNH AVNWGWQGTH 500
    EMNLRGAVMH VSPSGKTIVM QIHAVMPDGS NAPPLVKGQF YKNTLDFLVK 550
    NSAAGGKDTH YVFEGIELGK PYDAQIKVVD GVLSMTVNGQ TKTVDFVAKD 600
    AGWKDLKFYF KAGNYLQDRQ ADGSDTSALV KLYKLDVKHS S 641
    Length:641
    Mass (Da):71,003
    Last modified:October 1, 2000 - v1
    Checksum:i6C3C655070B7AD01
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB011415 Genomic DNA. Translation: BAB03312.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB011415 Genomic DNA. Translation: BAB03312.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HV6 X-ray 2.00 A 54-402 [» ]
    1QAZ X-ray 1.78 A 54-402 [» ]
    4E1Y X-ray 2.10 A/B 54-404 [» ]
    4F10 X-ray 2.20 A/B 54-404 [» ]
    4F13 X-ray 2.21 A/B 54-404 [» ]
    ProteinModelPortali Q9KWU1.
    SMRi Q9KWU1. Positions 53-402, 415-641.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi PL5. Polysaccharide Lyase Family 5.
    PL7. Polysaccharide Lyase Family 7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q9KWU1.

    Family and domain databases

    Gene3Di 1.50.10.110. 1 hit.
    2.60.120.200. 1 hit.
    InterProi IPR014895. Alginate_lyase_2.
    IPR008397. Alginate_lyase_dom.
    IPR008929. Chondroitin_lyas.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view ]
    Pfami PF05426. Alginate_lyase. 1 hit.
    PF08787. Alginate_lyase2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48230. SSF48230. 1 hit.
    SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Crystal structure of alginate lyase A1-III from Sphingomonas species A1 at 1.78 A resolution."
      Yoon H.J., Mikami B., Hashimoto W., Murata K.
      J. Mol. Biol. 290:505-514(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 54-402, ACTIVE SITE.
    2. "A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules."
      Momma K., Okamoto M., Mishima Y., Mori S., Hashimoto W., Murata K.
      J. Bacteriol. 182:3998-4004(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: A1Imported.
    3. "Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution."
      Yoon H.J., Hashimoto W., Miyake O., Murata K., Mikami B.
      J. Mol. Biol. 307:9-16(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 54-402.
    4. "Induced-fit motion of a lid loop involved in catalysis in alginate lyase A1-III."
      Mikami B., Ban M., Suzuki S., Yoon H.J., Miyake O., Yamasaki M., Ogura K., Maruyama Y., Hashimoto W., Murata K.
      Acta Crystallogr. D 68:1207-1216(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 54-404.

    Entry informationi

    Entry nameiQ9KWU1_SPHSX
    AccessioniPrimary (citable) accession number: Q9KWU1
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 1, 2000
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3