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Protein

Iron-dependent extradiol dioxygenase

Gene

hsaC

Organism
Rhodococcus jostii (strain RHA1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the meta-cleavage of 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) to produce 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA). Also involved in biphenyl and polychlorinated biphenyls (PCBs) degradation.2 Publications

Catalytic activityi

3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 = 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate.

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity

Pathwayi: cholesterol metabolism

This protein is involved in the pathway cholesterol metabolism, which is part of Steroid metabolism.
View all proteins of this organism that are known to be involved in the pathway cholesterol metabolism and in Steroid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi145IronBy similarity1
Binding sitei200SubstrateBy similarity1
Metal bindingi215IronBy similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei250SubstrateBy similarity1
Binding sitei256SubstrateBy similarity1
Metal bindingi266IronBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism, Cholesterol metabolism, Lipid degradation, Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16964.
UniPathwayiUPA00296.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-dependent extradiol dioxygenase (EC:1.13.11.25)
Gene namesi
Name:hsaC
Synonyms:bphC5
Ordered Locus Names:RHA1_ro04541
OrganismiRhodococcus jostii (strain RHA1)
Taxonomic identifieri101510 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus
Proteomesi
  • UP000008710 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene grow on cholesterol at a rate that is 60% that of the wild-type and develop a pink color consistent with the accumulation and nonenzymatic oxidation of a catechol.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004045081 – 300Iron-dependent extradiol dioxygenaseAdd BLAST300

Proteomic databases

PRIDEiQ9KWQ5.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi101510.RHA1_ro04541.

Structurei

3D structure databases

ProteinModelPortaliQ9KWQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105H37. Bacteria.
COG0346. LUCA.
HOGENOMiHOG000052193.
KOiK16049.
OMAiKMSATLG.
OrthoDBiPOG091H0B2E.

Family and domain databases

Gene3Di3.10.180.10. 2 hits.
InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR000486. Xdiol_ring_cleave_dOase_1/2.
[Graphical view]
PfamiPF00903. Glyoxalase. 1 hit.
[Graphical view]
SUPFAMiSSF54593. SSF54593. 2 hits.
PROSITEiPS00082. EXTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KWQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIRSLAYMR IEATDMSAWR EYGLKVLGMV EGKGSDPDAL YLRMDDFPAR
60 70 80 90 100
LVIFPGEHDR LSVSGWETAN AAELQEVRDN LSAAGVAFKE GTAEQLQDRR
110 120 130 140 150
VDELITFEDP SGNTLEAFHG AALEHRRVVS PYGHKFVTGE QGLGHVVLST
160 170 180 190 200
TDDEASLRFY RDVLGFRLRD SMRLPPQLVG RPADGKPAWL RFFGCNPRHH
210 220 230 240 250
SLAFLPMPTP SGIVHLMIEV ENSDDVGLCL DRALRKKVKM SATLGRHVND
260 270 280 290 300
LMLSFYMKTP GGFDIEFGCE GRQVEDESWI ARESTAVSLW GHDFSVGMQP
Length:300
Mass (Da):33,515
Last modified:October 1, 2000 - v1
Checksum:i6F46E2DDCC986C2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000431 Genomic DNA. Translation: ABG96327.1.
AB030672 Genomic DNA. Translation: BAA98137.1.
RefSeqiWP_011596919.1. NC_008268.1.

Genome annotation databases

EnsemblBacteriaiABG96327; ABG96327; RHA1_ro04541.
GeneIDi4222092.
KEGGirha:RHA1_ro04541.
PATRICi23207985. VBIRhoJos26306_4577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000431 Genomic DNA. Translation: ABG96327.1.
AB030672 Genomic DNA. Translation: BAA98137.1.
RefSeqiWP_011596919.1. NC_008268.1.

3D structure databases

ProteinModelPortaliQ9KWQ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi101510.RHA1_ro04541.

Proteomic databases

PRIDEiQ9KWQ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG96327; ABG96327; RHA1_ro04541.
GeneIDi4222092.
KEGGirha:RHA1_ro04541.
PATRICi23207985. VBIRhoJos26306_4577.

Phylogenomic databases

eggNOGiENOG4105H37. Bacteria.
COG0346. LUCA.
HOGENOMiHOG000052193.
KOiK16049.
OMAiKMSATLG.
OrthoDBiPOG091H0B2E.

Enzyme and pathway databases

UniPathwayiUPA00296.
BioCyciMetaCyc:MONOMER-16964.

Family and domain databases

Gene3Di3.10.180.10. 2 hits.
InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR000486. Xdiol_ring_cleave_dOase_1/2.
[Graphical view]
PfamiPF00903. Glyoxalase. 1 hit.
[Graphical view]
SUPFAMiSSF54593. SSF54593. 2 hits.
PROSITEiPS00082. EXTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHSAC_RHOJR
AccessioniPrimary (citable) accession number: Q9KWQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.