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Protein

Actinohivin

Gene

ath

Organism
Actinomycete sp. (strain K97-0003)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antiviral protein

Keywords - Ligandi

Lectin

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Actinohivin
Gene namesi
Name:ath
OrganismiActinomycete sp. (strain K97-0003)Curated
Taxonomic identifieri237531 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinomycetales

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51I → A: Partial loss of HIV fusion inhibition. 1 Publication1
Mutagenesisi79Q → A: Partial loss of HIV fusion inhibition. 1 Publication1
Mutagenesisi117Q → A: Partial loss of HIV fusion inhibition. 1 Publication1
Mutagenesisi155Q → A: Complete loss of HIV fusion inhibition. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
PropeptideiPRO_000002065327 – 46Sequence analysis2 PublicationsAdd BLAST20
ChainiPRO_000002065447 – 160ActinohivinAdd BLAST114

Interactioni

Subunit structurei

Interacts with HIV gp120 and SIV gp130.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
envQ757602EBI-8453511,EBI-8453491From a different organism.

Protein-protein interaction databases

IntActiQ9KWN0. 1 interactor.
MINTiMINT-8414661.

Structurei

Secondary structure

1160
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi46 – 49Combined sources4
Beta strandi51 – 53Combined sources3
Turni54 – 56Combined sources3
Beta strandi59 – 62Combined sources4
Beta strandi68 – 71Combined sources4
Helixi77 – 79Combined sources3
Beta strandi81 – 83Combined sources3
Beta strandi85 – 87Combined sources3
Turni92 – 94Combined sources3
Beta strandi97 – 100Combined sources4
Beta strandi105 – 109Combined sources5
Helixi115 – 117Combined sources3
Beta strandi119 – 121Combined sources3
Beta strandi127 – 129Combined sources3
Turni130 – 132Combined sources3
Beta strandi135 – 138Combined sources4
Beta strandi144 – 147Combined sources4
Helixi153 – 155Combined sources3
Beta strandi157 – 159Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A07X-ray1.19A/B43-160[»]
4DENX-ray1.60A47-160[»]
4ENDX-ray1.90A47-160[»]
4G1RX-ray1.57A/C47-160[»]
4P6AX-ray1.40A47-160[»]
ProteinModelPortaliQ9KWN0.
SMRiQ9KWN0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KWN0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 160Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST116
Repeati47 – 8411 PublicationAdd BLAST38
Repeati85 – 12221 PublicationAdd BLAST38
Repeati123 – 16031 PublicationAdd BLAST38

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni47 – 1603 X 38 AA tandem repeatsAdd BLAST114

Sequence similaritiesi

Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

Repeat, Signal

Family and domain databases

InterProiIPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9KWN0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTLTKLTIG AVALTGSFLA AAPASAAPAA DTTASPALGS QVSAQFASVT
60 70 80 90 100
IRNAQTGRLL DSNYNGNVYT LPANGGNYQR WTGPGDGTVR NAQTGRCLDS
110 120 130 140 150
NYDGAVYTLP CNGGSYQKWL FYSNGYIQNV ETGRVLDSNY NGNVYTLPAN
160
GGNYQKWYTG
Length:160
Mass (Da):16,870
Last modified:October 1, 2000 - v1
Checksum:i37F269BD16106240
GO

Mass spectrometryi

Molecular mass is 12520.3 Da from positions 47 - 160. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032371 Genomic DNA. Translation: BAA97578.1.
PIRiJC7622.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032371 Genomic DNA. Translation: BAA97578.1.
PIRiJC7622.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A07X-ray1.19A/B43-160[»]
4DENX-ray1.60A47-160[»]
4ENDX-ray1.90A47-160[»]
4G1RX-ray1.57A/C47-160[»]
4P6AX-ray1.40A47-160[»]
ProteinModelPortaliQ9KWN0.
SMRiQ9KWN0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9KWN0. 1 interactor.
MINTiMINT-8414661.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KWN0.

Family and domain databases

InterProiIPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAHV_ACTSK
AccessioniPrimary (citable) accession number: Q9KWN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Anti-HIV protein that inhibits virus membrane fusion.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.