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Protein

Lantibiotic nukacin

Gene

nukA

Organism
Staphylococcus warneri
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria, such as Lactobacillus sakei, Leuconostoc mesenteroides and Pediococcus pentosaceus. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin, Lantibiotic

Protein family/group databases

TCDBi1.C.21.1.5. the lacticin 481 (lacticin 481) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Lantibiotic nukacin
Alternative name(s):
Bacteriocin ISK-1
Gene namesi
Name:nukA
Encoded oniPlasmid pPI-10 Publication
OrganismiStaphylococcus warneri
Taxonomic identifieri1292 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 30302 PublicationsPRO_0000307094Add
BLAST
Peptidei31 – 5727Lantibiotic nukacinPRO_0000307095Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki39 ↔ 44Beta-methyllanthionine (Thr-Cys)Curated
Cross-linki41 ↔ 55Lanthionine (Ser-Cys)Curated
Cross-linki48 ↔ 56Lanthionine (Ser-Cys)Curated
Modified residuei54 – 5412,3-didehydrobutyrineCurated

Post-translational modificationi

Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.

Keywords - PTMi

Thioether bond

Family & Domainsi

Sequence similaritiesi

Belongs to the type A lantibiotic family.Curated

Family and domain databases

InterProiIPR007682. Lantibiotic_typ-A_Lactobact.
[Graphical view]
PfamiPF04604. L_biotic_typeA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9KWM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENSKVMKDI EVANLLEEVQ EDELNEVLGA KKKSGVIPTV SHDCHMNSFQ

FVFTCCS
Length:57
Mass (Da):6,403
Last modified:October 1, 2000 - v1
Checksum:i9BF0046242995426
GO

Mass spectrometryi

Molecular mass is 2960.1 Da from positions 31 - 57. Determined by FAB. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB034941 Genomic DNA. Translation: BAA95674.1.
AB121757 Genomic DNA. Translation: BAC98759.1.
AB125341 Genomic DNA. Translation: BAD01007.1.
RefSeqiNP_940772.1. NC_005207.3.
WP_011152951.1. NC_005207.3.

Genome annotation databases

GeneIDi2648465.
KEGGipg:2648465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB034941 Genomic DNA. Translation: BAA95674.1.
AB121757 Genomic DNA. Translation: BAC98759.1.
AB125341 Genomic DNA. Translation: BAD01007.1.
RefSeqiNP_940772.1. NC_005207.3.
WP_011152951.1. NC_005207.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.21.1.5. the lacticin 481 (lacticin 481) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2648465.
KEGGipg:2648465.

Family and domain databases

InterProiIPR007682. Lantibiotic_typ-A_Lactobact.
[Graphical view]
PfamiPF04604. L_biotic_typeA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A novel lantibiotic, nukacin ISK-1, of Staphylococcus warneri ISK-1: cloning of the structural gene and identification of the structure."
    Sashihara T., Kimura H., Higuchi T., Adachi A., Matsusaki H., Sonomoto K., Ishizaki A.
    Biosci. Biotechnol. Biochem. 64:2420-2428(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-57, DEHYDRATION AT THR-39; SER-41; SER-48 AND THR-54, LANTHIONINE CROSS-LINKS.
    Strain: ISK-1.
  2. "Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1."
    Aso Y., Sashihara T., Nagao J., Kanemasa Y., Koga H., Hashimoto T., Higuchi T., Adachi A., Nomiyama H., Ishizaki A., Nakayama J., Sonomoto K.
    Biosci. Biotechnol. Biochem. 68:1663-1671(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ISK-1.
  3. "Description of complete DNA sequence of two plasmids from the nukacin ISK-1 producer, Staphylococcus warneri ISK-1."
    Aso Y., Koga H., Sashihara T., Nagao J., Kanemasa Y., Nakayama J., Sonomoto K.
    Plasmid 53:164-178(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ISK-1.
  4. "Purification and partial identification of bacteriocin ISK-1, a new lantibiotic produced by Pediococcus sp. ISK-1."
    Kimura H., Matsusaki H., Sashihara T., Sonomoto K., Ishizaki A.
    Biosci. Biotechnol. Biochem. 62:2341-2345(1998)
    Cited for: PROTEIN SEQUENCE OF 31-37, AMINO-ACID COMPOSITION, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: ISK-1.
  5. "Heterologous expression and functional analysis of the gene cluster for the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1."
    Aso Y., Nagao J., Koga H., Okuda K., Kanemasa Y., Sashihara T., Nakayama J., Sonomoto K.
    J. Biosci. Bioeng. 98:429-436(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
    Strain: ISK-1.
  6. "Lysine-oriented charges trigger the membrane binding and activity of nukacin ISK-1."
    Asaduzzaman S.M., Nagao J., Aso Y., Nakayama J., Sonomoto K.
    Appl. Environ. Microbiol. 72:6012-6017(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: ISK-1.

Entry informationi

Entry nameiLANNA_STAWA
AccessioniPrimary (citable) accession number: Q9KWM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 1, 2000
Last modified: March 16, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-7 is the initiator.Curated

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.