Reviewed, UniProtKB/Swiss-Prot Q9KWE5 (HUTI_AGRRH)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
ORF Names:	riorf39

Encoded on

Plasmid pRi1724

Organism

Agrobacterium rhizogenes

Taxonomic identifier

359 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential HAMAP MF_00372.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Imidazolonepropionase HAMAP MF_00372		PRO_0000160941
Sites
	Metal binding	72	1	Zinc or iron By similarity
	Metal binding	74	1	Zinc or iron By similarity
	Metal binding	242	1	Zinc or iron By similarity
	Metal binding	317	1	Zinc or iron By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	144	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KWE5-1 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: A618F937E658D4B3	FASTA	407	43,511
	10 20 30 40 50 60 MIGASKLIRI WINARVYPAI AGAEIINDAV IVAKEGRLTF VGPASALSID DRDAETIDCG 70 80 90 100 110 120 GRLITPGLVD CHTHIVFGGD RAMEFEMRLE GSTYEEVARA GGGIVSSVKA TNALTVDQLV 130 140 150 160 170 180 QASLPRVDTL LSEGVSTLEI KSGYGLTIEG ELNILRAARA LEKVRPVRVV TSYLAAHATP 190 200 210 220 230 240 PEYKGRHADY IADVVLPGMD RGHAEKLIDA VDGFCEGIAF TVDEMRRVFN HAKVLGIPVK 250 260 270 280 290 300 LHAEQLSNLG GAKMAASYGA LSADHLEYLD KEGAEAMAKA GTVAVILPGA FYAINETRKP 310 320 330 340 350 360 PIQTLRDAGV RMAIATDCNP GTSPLTSLLL TMNMSATLFR LTVEECICGA TREAARALGI 370 380 390 400 LDTTGTLEVG KSADLAIWNI ERPAELVYRI GFNPLHSRVF KGKQVST

References

[1]

"Analysis of unique variable region of a plant root inducing plasmid, pRi1724, by the construction of its physical map and library." Moriguchi K., Maeda Y., Satou M., Kataoka M., Tanaka N., Yoshida K. DNA Res. 7:157-163(2000) [PubMed: 10907845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MAFF03-01724.

[2]

"The complete nucleotide sequence of a plant root-inducing (Ri) plasmid indicates its chimeric structure and evolutionary relationship between tumor-inducing (Ti) and symbiotic (Sym) plasmids in Rhizobiaceae." Moriguchi K., Maeda Y., Satou M., Hardayani N.S.N., Kataoka M., Tanaka N., Yoshida K. J. Mol. Biol. 307:771-784(2001) [PubMed: 11273700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MAFF03-01724.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB039932 Genomic DNA. Translation: BAA97750.1. Different initiation. AP002086 Genomic DNA. Translation: BAB16158.1. Different initiation.
RefSeq	NP_066620.1.
3D structure databases
SMR	Q9KWE5. Positions 10-406.
ModBase	Search...
Genome annotation databases
GeneID	874842.
Enzyme and pathway databases
BRENDA	3.5.2.7. 2198.
Family and domain databases
HAMAP	MF_00372. HutI. [Tree]
InterPro	IPR013108. Amidohydro_3. IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
Pfam	PF07969. Amidohydro_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_AGRRH

Accession

Primary (citable) accession number: Q9KWE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)