Reviewed, UniProtKB/Swiss-Prot Q9KWA3 (ACSA_AGRRH)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase
    Acyl-activating enzyme

Gene names

Name:	acsA
ORF Names:	riorf81

Encoded on

Plasmid pRi1724

Organism

Agrobacterium rhizogenes

Taxonomic identifier

359 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	652 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Plasmid
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 652	652	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_0000208351
Sites
	Active site	517	1	By similarity
Amino acid modifications
	Modified residue	609	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KWA3-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5B97DF7EAB99A8FB	FASTA	652	71,992
	10 20 30 40 50 60 MSEKIYPVAK PVESHALINK AEYQEWYAES VADPEQFWGK HGKRIDWFKP YTSVKDTSFT 70 80 90 100 110 120 GDVSIKWFED GETNVSYNCI DRHLATNGDQ VAIIWEGDDP SLDRKITYRE LYEHVCRMAN 130 140 150 160 170 180 VLKKHGVKKG DRVTIYMPMV PEAAYAMLAC ARIGAIHSVV FGGFSPEALG GRIVDCQSTF 190 200 210 220 230 240 VITCDEGLRG GKPVPLKANV DKAIDIAARG HVMVKNVLVI RRTGSPLSWA PGRDLWHHEE 250 260 270 280 290 300 AATVSADCPP EPMKAEDPLF ILYTSGSTGK PKGVLHTTGG YLVYASMTHE YVFDYQHGDI 310 320 330 340 350 360 YWCTADVGWV TGHSYIVYGP LANCATTLMF EGIPTFPDAG RFWDVIDKHK VNIFYTAPTA 370 380 390 400 410 420 IRSLMGAGDD FVKRSSRSSL RLLGSVGEPI NPEAWEWYYH TVGDGRCPVV DTWWQTETGG 430 440 450 460 470 480 IMITPLPGAT DLKPGSATRP FFGIRPELVD AEGKLIEGAA DGNLCIADSW PGQARSVYGD 490 500 510 520 530 540 HERFIQTYFS TYEGKYFTGD GCRRDADGYY WITGRVDDVL NVSGHRLGTA EVESALVSHH 550 560 570 580 590 600 LVSEAAVVGY PHNIKGQGIY CYVTLMAGSE GSEELRQQLV KHVRAEIGPI ASPDKIQFAP 610 620 630 640 650 GLPKTRSGKI MRRILRKIAE DDFGSLGDTS TLADPAVVDD LIANRQNRAE AA

References

[1]

"Analysis of unique variable region of a plant root inducing plasmid, pRi1724, by the construction of its physical map and library." Moriguchi K., Maeda Y., Satou M., Kataoka M., Tanaka N., Yoshida K. DNA Res. 7:157-163(2000) [PubMed: 10907845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MAFF03-01724.

[2]

"The complete nucleotide sequence of a plant root-inducing (Ri) plasmid indicates its chimeric structure and evolutionary relationship between tumor-inducing (Ti) and symbiotic (Sym) plasmids in Rhizobiaceae." Moriguchi K., Maeda Y., Satou M., Hardayani N.S.N., Kataoka M., Tanaka N., Yoshida K. J. Mol. Biol. 307:771-784(2001) [PubMed: 11273700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MAFF03-01724.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB039932 Genomic DNA. Translation: BAA97792.1. AP002086 Genomic DNA. Translation: BAB16200.1.
RefSeq	NP_066662.1.
3D structure databases
HSSP	HSSP built from PDB template 1PG4 based on UniProtKB Q8ZKF6.
ModBase	Search...
Genome annotation databases
GeneID	874783.
Enzyme and pathway databases
BRENDA	6.2.1.1. 2198.
Family and domain databases
HAMAP	MF_01123. [Tree]
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_AGRRH

Accession

Primary (citable) accession number: Q9KWA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)