Q9KVZ8 (MTX1_XANVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Modification methylase XhoI Short name=M.XhoI EC=2.1.1.72 Alternative name(s): Adenine-specific methyltransferase XhoI
Organism	Xanthomonas vasicola
Taxonomic identifier	56459 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the XhoI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 546	546	Modification methylase XhoI		PRO_0000087984

Sequences

	Sequence		Length	Mass (Da)
	Q9KVZ8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D9EF9B9D4E137BD2	FASTA	546	61,025
	10 20 30 40 50 60 MPDERGAIFT RREVVEFILD LVGYTEDRDL AQTKLLEPSA GHADFLLPII GRLVRSYVAH 70 80 90 100 110 120 GGDLTRGAQT LGPAIRAYEV HESSLETARS VVVAELLRLG VKKAAADGLG RTWLVRADFL 130 140 150 160 170 180 MAPLPHTFDF VVGNPPYVRQ ELIPAVLLAR YRARFKTLYD RADLYIPFYE RCLDVLAPGG 190 200 210 220 230 240 RLGFICTDRW TKNKYGGPLR AMVSEEFSLT HFVDLVDTQA FLSNVMTYPA ITVIERPKPK 250 260 270 280 290 300 SKARPTRVAY RPAISAEVFG PLAKAMTGTK LNHKAGVVEM SGVVNGSEPW ILHQADRLAL 310 320 330 340 350 360 VRRLEETLPT LEEAGCKVGI GVATGNDGVY IGDMKTLNVE PSRKLPLART QDLRGGSIDW 370 380 390 400 410 420 QGKGVLNPFE EDGQVVDLAS YPKFAAYLQE HAIQIKARHV AKKNPERWFR TIDRIYPALA 430 440 450 460 470 480 KTPKLLVPDI KGDAHIVYEE GKLYPHHNLY FITANEWDLR ALQAVLMSGV ARLFVGTYST 490 500 510 520 530 540 TMRGGFLRFQ AQYLRRIRVP HWKNVPKPLQ KALREAAVAG DREAANRATY QLYGLNEAER DIVATV

References

[1]	"Nucleotide sequences of the XhoI methylase and endonuclease genes." Morita M., Sugino Y. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13461 / XH 3 / LMG 7416.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB046567 Genomic DNA. Translation: BAB03596.1.
3D structure databases
ProteinModelPortal	Q9KVZ8.
ModBase	Search...
Protein family/group databases
REBASE	3530. M.XhoI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. IPR011639. RM_methylase_Eco57I. IPR025931. TaqI_C. [Graphical view]
Pfam	PF07669. Eco57I. 1 hit. PF12950. TaqI_C. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTX1_XANVA

Accession

Primary (citable) accession number: Q9KVZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 1, 2000
Last modified:	July 11, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program