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Protein

Type-2 restriction enzyme XhoI

Gene
N/A
Organism
Xanthomonas vasicola
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi2136. XhoI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme XhoI (EC:3.1.21.4)
Short name:
R.XhoI
Alternative name(s):
Endonuclease XhoI
Type II restriction enzyme XhoI
OrganismiXanthomonas vasicola
Taxonomic identifieri56459 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248Type-2 restriction enzyme XhoIPRO_0000077372Add
BLAST

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR007636. Restrct_endonuc_II_XhoI.
[Graphical view]
PfamiPF04555. XhoI. 1 hit.
[Graphical view]
PIRSFiPIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KVZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALDLAEYDR LARLGVAQFW DGRSSALEND EERSQGGERS GVLGGRNMDG
60 70 80 90 100
FLAMIEGIVR KNGLPDAEVC IKGRPNLTLP GYYRPTKLWD VLVFDGKKLV
110 120 130 140 150
AAVELKSHVG PSFGNNFNNR AEEAIGTAHD LATAIREGIL GDQLPPFTGW
160 170 180 190 200
LILVEDCEKS KRAVRDSSPH FPVFPDFKGA SYLTRYEVLC RKLILKGFTP
210 220 230 240
RPQSLLRPAL RVLGATIASF RKPRVCAHLR HGWPAMYPVG QSRIRVNF
Length:248
Mass (Da):27,575
Last modified:October 1, 2000 - v1
Checksum:i81D3B2B231BE6622
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046567 Genomic DNA. Translation: BAB03597.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046567 Genomic DNA. Translation: BAB03597.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL2439945.

Protein family/group databases

REBASEi2136. XhoI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR007636. Restrct_endonuc_II_XhoI.
[Graphical view]
PfamiPF04555. XhoI. 1 hit.
[Graphical view]
PIRSFiPIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequences of the XhoI methylase and endonuclease genes."
    Morita M., Sugino Y.
    Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13461 / XH 3 / LMG 7416.

Entry informationi

Entry nameiT2X1_XANVA
AccessioniPrimary (citable) accession number: Q9KVZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 1, 2000
Last modified: October 1, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.