Q9KVZ7 (T2X1_XANVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Type-2 restriction enzyme XhoI Short name=R.XhoI EC=3.1.21.4 Alternative name(s): Endonuclease XhoI Type II restriction enzyme XhoI
Organism	Xanthomonas vasicola
Taxonomic identifier	56459 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Sequence similarities	Belongs to the type II restriction enzyme XhoI family.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Type-2 restriction enzyme XhoI		PRO_0000077372

Sequences

	Sequence		Length	Mass (Da)
	Q9KVZ7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 81D3B2B231BE6622	FASTA	248	27,575
	10 20 30 40 50 60 MALDLAEYDR LARLGVAQFW DGRSSALEND EERSQGGERS GVLGGRNMDG FLAMIEGIVR 70 80 90 100 110 120 KNGLPDAEVC IKGRPNLTLP GYYRPTKLWD VLVFDGKKLV AAVELKSHVG PSFGNNFNNR 130 140 150 160 170 180 AEEAIGTAHD LATAIREGIL GDQLPPFTGW LILVEDCEKS KRAVRDSSPH FPVFPDFKGA 190 200 210 220 230 240 SYLTRYEVLC RKLILKGFTP RPQSLLRPAL RVLGATIASF RKPRVCAHLR HGWPAMYPVG QSRIRVNF

References

[1]	"Nucleotide sequences of the XhoI methylase and endonuclease genes." Morita M., Sugino Y. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13461 / XH 3 / LMG 7416.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB046567 Genomic DNA. Translation: BAB03597.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	2136. XhoI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007636. Restrct_endonuc_II_XhoI. [Graphical view]
Pfam	PF04555. XhoI. 1 hit. [Graphical view]
PIRSF	PIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T2X1_XANVA

Accession

Primary (citable) accession number: Q9KVZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 1, 2000
Last modified:	July 11, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program