Q9KVW7 (SYGA_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	VC_0021

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Sequence caution	The sequence AAF93199.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_0000072878

Sequences

	Sequence		Length	Mass (Da)
	Q9KVW7 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 23AA9D1E5DEC742E	FASTA	305	35,038
	10 20 30 40 50 60 MQKYDIKTFQ GMILALQDYW AQQGCTIVQP LDMEVGAGTS HPMTCLRALG PEPIATAYVQ 70 80 90 100 110 120 PSRRPTDGRY GENPNRLQHY YQFQVIIKPS PDNIQELYLG SLRVLGVDPC VHDIRFVEDN 130 140 150 160 170 180 WENPTLGAWG LGWEVWLNGM EVTQFTYFQQ VGGLECKPVT GEITYGIERL AMYIQGVDSV 190 200 210 220 230 240 YDLVWTDGPL GKVTYGDIFH QNEVEQSTYN FEHADVDFLF TYFDQCEKEC KYLLELEKPL 250 260 270 280 290 300 PLPAYERILK AAHAFNLLDA RKAISVTERQ RYILRIRNLT KSVAEAYYAS REALGFPMCK KSEQK

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF93199.1. Different initiation.
PIR	G82373.
RefSeq	NP_229680.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KVW7.
SMR	Q9KVW7. Positions 9-287.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2614961.
GenomeReviews	Gene locus VC_0021 in contig AE003852_GR.
KEGG	vch:VC0021.
PATRIC	20079090. VBIVibCho83274_0020.
TIGR	VC_0021.
Phylogenomic databases
HOGENOM	HBG285316.
OMA	CRRPTDG.
PhylomeDB	Q9KVW7.
ProtClustDB	PRK09348.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_VIBCH

Accession

Primary (citable) accession number: Q9KVW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program