Q9KVT7 (PURE_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N5-carboxyaminoimidazole ribonucleotide mutase
Short name=N5-CAIR mutase
EC=5.4.99.18

Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide mutase

Gene names

Name:	purE
Ordered Locus Names:	VC_0052

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR) By similarity.
Catalytic activity	5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the AIR carboxylase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	5-(carboxyamino)imidazole ribonucleotide mutase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	N5-carboxyaminoimidazole ribonucleotide mutase		PRO_0000074981
Sites
	Binding site	9	1	Substrate By similarity
	Binding site	12	1	Substrate By similarity
	Binding site	39	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KVT7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5E843DF107809FCB	FASTA	161	16,645
	10 20 30 40 50 60 MTVGIIMGSK SDWPTMKHAA EMLDQFGVAY ETKVVSAHRT PHLLADYASS AKERGLQVII 70 80 90 100 110 120 AGAGGAAHLP GMTAAFTSLP VLGVPVQSRA LSGLDSLYSI VQMPKGIAVG TLAIGEAGAA 130 140 150 160 NAGLLAAQII GIHNPEVMSK VEAFRAKQTQ SVLDNPNPAE E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of two Vibrio cholerae El Tor genes induced in the estuarine environment." Soares C.A.G., DiRita V.J., Santos E.O., Coelho A. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: El Tor C3294.
[2]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF459402 Genomic DNA. Translation: AAL66729.1. AE003852 Genomic DNA. Translation: AAF93230.1.
PIR	C82370.
RefSeq	NP_229711.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KVT7.
SMR	Q9KVT7. Positions 3-145.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2614427.
GenomeReviews	Gene locus VC_0052 in contig AE003852_GR.
KEGG	vch:VC0052.
PATRIC	20079152. VBIVibCho83274_0051.
TIGR	VC_0052.
Phylogenomic databases
HOGENOM	HBG301965.
OMA	LIMGSDS.
PhylomeDB	Q9KVT7.
ProtClustDB	CLSK2391028.
Family and domain databases
InterPro	IPR024694. N5-CAIR_mutase_PurE. IPR000031. N5-CAIR_Mutase_PurE_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit.
KO	K01588.
Pfam	PF00731. AIRC. 1 hit. [Graphical view]
PIRSF	PIRSF001338. AIR_carboxylase. 1 hit.
SMART	SM01001. AIRC. 1 hit. [Graphical view]
SUPFAM	SSF52255. AIR_carboxyl. 1 hit.
TIGRFAMs	TIGR01162. PurE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURE_VIBCH

Accession

Primary (citable) accession number: Q9KVT7
Secondary accession number(s): Q7B8Z7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program