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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei265-phosphoribose 1-diphosphateUniRule annotation1
Binding sitei995-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei1005-phosphoribose 1-diphosphateUniRule annotation1
Binding sitei1035-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei1055-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei128OrotateUniRule annotation1
Binding sitei156OrotateUniRule annotation1

GO - Molecular functioni

  • magnesium ion binding Source: UniProtKB-HAMAP
  • orotate phosphoribosyltransferase activity Source: TIGR

GO - Biological processi

  • 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  • pyrimidine ribonucleotide biosynthetic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:VC_0211
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001107651 – 213Orotate phosphoribosyltransferaseAdd BLAST213

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243277.VC0211.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 14Combined sources12
Beta strandi17 – 24Combined sources8
Beta strandi26 – 28Combined sources3
Beta strandi30 – 35Combined sources6
Helixi37 – 39Combined sources3
Helixi43 – 60Combined sources18
Beta strandi65 – 69Combined sources5
Turni71 – 73Combined sources3
Helixi74 – 88Combined sources15
Beta strandi94 – 98Combined sources5
Beta strandi111 – 114Combined sources4
Beta strandi118 – 123Combined sources6
Beta strandi128 – 130Combined sources3
Helixi131 – 142Combined sources12
Beta strandi146 – 155Combined sources10
Beta strandi161 – 165Combined sources5
Helixi166 – 174Combined sources9
Beta strandi177 – 183Combined sources7
Helixi184 – 192Combined sources9
Helixi197 – 211Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N2LX-ray2.10A/B/C/D/E/F/G/H1-213[»]
ProteinModelPortaliQ9KVD5.
SMRiQ9KVD5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KVD5.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 35Orotate bindingUniRule annotation2
Regioni72 – 735-phosphoribose 1-diphosphate bindingUniRule annotation2
Regioni124 – 1325-phosphoribose 1-diphosphate bindingUniRule annotation9

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP2. Bacteria.
COG0461. LUCA.
KOiK00762.
OMAiMKAYQRQ.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KVD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAYQREFIE FALEKQVLKF GEFTLKSGRK SPYFFNAGLF NTGRDLARLG
60 70 80 90 100
RFYAAALVDS GIEFDVLFGP AYKGIPIATT TAVALADHHD VDTPYCFNRK
110 120 130 140 150
EAKNHGEGGN LVGSKLEGRV MLVDDVITAG TAIRESMELI QANKADLAGV
160 170 180 190 200
LVAIDRQEKG KGELSAIQEV ERDFGCAVIS IVSLTDLITY LEQQGNNTEH
210
LEAVKAYRAQ YGI
Length:213
Mass (Da):23,402
Last modified:March 29, 2005 - v2
Checksum:i71A3E2A3451BEE82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93387.1.
PIRiE82350.
RefSeqiNP_229868.2. NC_002505.1.
WP_001884129.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93387; AAF93387; VC_0211.
GeneIDi2614710.
KEGGivch:VC0211.
PATRICi20079466. VBIVibCho83274_0192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93387.1.
PIRiE82350.
RefSeqiNP_229868.2. NC_002505.1.
WP_001884129.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N2LX-ray2.10A/B/C/D/E/F/G/H1-213[»]
ProteinModelPortaliQ9KVD5.
SMRiQ9KVD5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0211.

Protocols and materials databases

DNASUi2614710.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93387; AAF93387; VC_0211.
GeneIDi2614710.
KEGGivch:VC0211.
PATRICi20079466. VBIVibCho83274_0192.

Phylogenomic databases

eggNOGiENOG4107QP2. Bacteria.
COG0461. LUCA.
KOiK00762.
OMAiMKAYQRQ.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.

Miscellaneous databases

EvolutionaryTraceiQ9KVD5.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRE_VIBCH
AccessioniPrimary (citable) accession number: Q9KVD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.