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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 2615-phosphoribose 1-diphosphateUniRule annotation
Binding sitei99 – 9915-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei100 – 10015-phosphoribose 1-diphosphateUniRule annotation
Binding sitei103 – 10315-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei105 – 10515-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei128 – 1281OrotateUniRule annotation
Binding sitei156 – 1561OrotateUniRule annotation

GO - Molecular functioni

  • magnesium ion binding Source: UniProtKB-HAMAP
  • orotate phosphoribosyltransferase activity Source: TIGR

GO - Biological processi

  • 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  • pyrimidine ribonucleotide biosynthetic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:VC_0211
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Orotate phosphoribosyltransferasePRO_0000110765Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243277.VC0211.

Structurei

Secondary structure

1
213
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412Combined sources
Beta strandi17 – 248Combined sources
Beta strandi26 – 283Combined sources
Beta strandi30 – 356Combined sources
Helixi37 – 393Combined sources
Helixi43 – 6018Combined sources
Beta strandi65 – 695Combined sources
Turni71 – 733Combined sources
Helixi74 – 8815Combined sources
Beta strandi94 – 985Combined sources
Beta strandi111 – 1144Combined sources
Beta strandi118 – 1236Combined sources
Beta strandi128 – 1303Combined sources
Helixi131 – 14212Combined sources
Beta strandi146 – 15510Combined sources
Beta strandi161 – 1655Combined sources
Helixi166 – 1749Combined sources
Beta strandi177 – 1837Combined sources
Helixi184 – 1929Combined sources
Helixi197 – 21115Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3N2LX-ray2.10A/B/C/D/E/F/G/H1-213[»]
ProteinModelPortaliQ9KVD5.
SMRiQ9KVD5. Positions 1-213.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KVD5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 352Orotate bindingUniRule annotation
Regioni72 – 7325-phosphoribose 1-diphosphate bindingUniRule annotation
Regioni124 – 13295-phosphoribose 1-diphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP2. Bacteria.
COG0461. LUCA.
KOiK00762.
OMAiMKAYQRQ.
OrthoDBiEOG6091H8.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KVD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAYQREFIE FALEKQVLKF GEFTLKSGRK SPYFFNAGLF NTGRDLARLG
60 70 80 90 100
RFYAAALVDS GIEFDVLFGP AYKGIPIATT TAVALADHHD VDTPYCFNRK
110 120 130 140 150
EAKNHGEGGN LVGSKLEGRV MLVDDVITAG TAIRESMELI QANKADLAGV
160 170 180 190 200
LVAIDRQEKG KGELSAIQEV ERDFGCAVIS IVSLTDLITY LEQQGNNTEH
210
LEAVKAYRAQ YGI
Length:213
Mass (Da):23,402
Last modified:March 29, 2005 - v2
Checksum:i71A3E2A3451BEE82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93387.1.
PIRiE82350.
RefSeqiNP_229868.2. NC_002505.1.
WP_001884129.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93387; AAF93387; VC_0211.
GeneIDi2614710.
KEGGivch:VC0211.
PATRICi20079466. VBIVibCho83274_0192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93387.1.
PIRiE82350.
RefSeqiNP_229868.2. NC_002505.1.
WP_001884129.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3N2LX-ray2.10A/B/C/D/E/F/G/H1-213[»]
ProteinModelPortaliQ9KVD5.
SMRiQ9KVD5. Positions 1-213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0211.

Protocols and materials databases

DNASUi2614710.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93387; AAF93387; VC_0211.
GeneIDi2614710.
KEGGivch:VC0211.
PATRICi20079466. VBIVibCho83274_0192.

Phylogenomic databases

eggNOGiENOG4107QP2. Bacteria.
COG0461. LUCA.
KOiK00762.
OMAiMKAYQRQ.
OrthoDBiEOG6091H8.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.

Miscellaneous databases

EvolutionaryTraceiQ9KVD5.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiPYRE_VIBCH
AccessioniPrimary (citable) accession number: Q9KVD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: March 29, 2005
Last modified: November 11, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.