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Q9KV81 (PUR2_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:VC_0275
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151497

Regions

Domain109 – 316208ATP-grasp
Nucleotide binding135 – 19662ATP By similarity

Sites

Metal binding2861Magnesium or manganese By similarity
Metal binding2881Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KV81 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 8801E6FE43DD3851

FASTA42945,308
        10         20         30         40         50         60 
MQVLIIGSGG REHALAWKVA QNPQVDTIYV APGNAGTALE HKVQNVNIGI TDIPALVAFA 

        70         80         90        100        110        120 
QDKAIELTIV GPEAPLVIGV VDAFRAAGLP IFGPTQGAAQ LEGSKAFTKD FLARHNIPTA 

       130        140        150        160        170        180 
AYANFTEIEP ALAYVREKGA PIVVKADGLA AGKGVIVAMT LQEAEDAIQD MLAGNAFGSA 

       190        200        210        220        230        240 
GSRVVVEEFL DGEEASFIVM VDGENVLPMA TSQDHKRVGD ADTGPNTGGM GAYSPAPVVT 

       250        260        270        280        290        300 
QDVHDRVMRE VIDPTVRGMA AEGNTYTGFL YAGLMIDSTG APKVIEYNCR FGDPETQPIM 

       310        320        330        340        350        360 
MRLQSDLVEL CQAAIAGKLD QVESKWDPRA SIGVVLAAGG YPGDYAKGEV ISGLPTQESA 

       370        380        390        400        410        420 
GQKVFHAGTE TQGDQVVTNG GRVLCATALG NTVLEAQQRA YQLADQIHWN GMFCRRDIGY 


RAIAREQAK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE003852 Genomic DNA. Translation: AAF93450.1.
PIRB82344.
RefSeqNP_229931.1. NC_002505.1.

3D structure databases

ProteinModelPortalQ9KV81.
SMRQ9KV81. Positions 1-425.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243277.VC0275.

Protocols and materials databases

DNASU2614998.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF93450; AAF93450; VC_0275.
GeneID2614998.
KEGGvch:VC0275.
PATRIC20079593. VBIVibCho83274_0255.

Phylogenomic databases

eggNOGCOG0151.
KOK01945.
OMAYKGFLYA.
OrthoDBEOG69SKD1.

Enzyme and pathway databases

BioCycVCHO:VC0275-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_VIBCH
AccessionPrimary (citable) accession number: Q9KV81
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways