Q9KV81 (PUR2_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	VC_0275

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro purine ribonucleotide biosynthetic process Inferred from sequence or structural similarity Ref.1. Source: TIGR
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from sequence or structural similarity Ref.1. Source: TIGR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138		PRO_0000151497
Regions
	Domain	109 – 316	208	ATP-grasp
	Nucleotide binding	135 – 196	62	ATP By similarity
Sites
	Metal binding	286	1	Magnesium or manganese By similarity
	Metal binding	288	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KV81 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8801E6FE43DD3851	FASTA	429	45,308
	10 20 30 40 50 60 MQVLIIGSGG REHALAWKVA QNPQVDTIYV APGNAGTALE HKVQNVNIGI TDIPALVAFA 70 80 90 100 110 120 QDKAIELTIV GPEAPLVIGV VDAFRAAGLP IFGPTQGAAQ LEGSKAFTKD FLARHNIPTA 130 140 150 160 170 180 AYANFTEIEP ALAYVREKGA PIVVKADGLA AGKGVIVAMT LQEAEDAIQD MLAGNAFGSA 190 200 210 220 230 240 GSRVVVEEFL DGEEASFIVM VDGENVLPMA TSQDHKRVGD ADTGPNTGGM GAYSPAPVVT 250 260 270 280 290 300 QDVHDRVMRE VIDPTVRGMA AEGNTYTGFL YAGLMIDSTG APKVIEYNCR FGDPETQPIM 310 320 330 340 350 360 MRLQSDLVEL CQAAIAGKLD QVESKWDPRA SIGVVLAAGG YPGDYAKGEV ISGLPTQESA 370 380 390 400 410 420 GQKVFHAGTE TQGDQVVTNG GRVLCATALG NTVLEAQQRA YQLADQIHWN GMFCRRDIGY RAIAREQAK

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF93450.1.
PIR	B82344.
RefSeq	NP_229931.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KV81.
SMR	Q9KV81. Positions 1-425.
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VC0275.
Protocols and materials databases
DNASU	2614998.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF93450; AAF93450; VC_0275.
GeneID	2614998.
KEGG	vch:VC0275.
PATRIC	20079593. VBIVibCho83274_0255.
Phylogenomic databases
eggNOG	COG0151.
KO	K01945.
OMA	MGAYTPL.
ProtClustDB	PRK00885.
Enzyme and pathway databases
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_VIBCH

Accession

Primary (citable) accession number: Q9KV81

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program