Q9KV80 (PUR9_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
   EC=2.1.2.3
   Alternative name(s):
   AICAR transformylase
2. IMP cyclohydrolase
   EC=3.5.4.10
   Alternative name(s):
   ATIC
   IMP synthase
   Inosinicase

Gene names

Name:	purH
Ordered Locus Names:	VC_0276

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139
Sequence similarities	Belongs to the PurH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine ribonucleotide biosynthetic process Inferred from sequence or structural similarity Ref.1. Source: TIGR
Molecular_function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from sequence or structural similarity Ref.1. Source: TIGR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139		PRO_0000192144

Sequences

	Sequence		Length	Mass (Da)
	Q9KV80 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: BE64A6D4C42FA617	FASTA	530	57,327
	10 20 30 40 50 60 MNNARPIHRA LLSVSDKTGI VEFAKALAER GVELLSTGGT ARLLAEQGLT VTEVSDYTGF 70 80 90 100 110 120 PEMMDGRVKT LHPKVHGGIL GRRGQDDAVM NTHGIQPIDM VVVNLYPFAQ TVANPNCTLA 130 140 150 160 170 180 DAVENIDIGG PTMVRSAAKN HKDVAIVVNA HDYDRVIREM DANHNSLTLA TRFDLAIAAF 190 200 210 220 230 240 EHTAAYDGMI ANYFGTLVPS YGDNKEGDEE SKFPRTFNAQ FIKKQDMRYG ENSHQAAAFY 250 260 270 280 290 300 VEANPQEASV ATARQIQGKA LSYNNIADTD AALECVKEFS EPACVIVKHA NPCGVALGDD 310 320 330 340 350 360 LLQAYNRAYQ TDPTSAFGGI IAFNRELDGE TARAIIERQF VEVIIAPKVS QAAIDIVAAK 370 380 390 400 410 420 QNVRLLECGE WQGQTTGFDL KRVNGGLLVQ DRDQGMVAQD DLQVVSTRQP SDAELKDALF 430 440 450 460 470 480 CWKVAKYVKS NAIVYAKGDM TIGIGAGQMS RVYSAKIAGI KAADEGLEVA GSVMASDAFF 490 500 510 520 530 PFRDGIDAAA EAGITCVIQP GGSMRDQEVI DAANEHGMAM IFTGMRHFRH

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF93451.1.
PIR	C82344.
RefSeq	NP_229932.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KV80.
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VC0276.
Protocols and materials databases
DNASU	2614999.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF93451; AAF93451; VC_0276.
GeneID	2614999.
KEGG	vch:VC0276.
PATRIC	20079595. VBIVibCho83274_0256.
Phylogenomic databases
eggNOG	COG0138.
KO	K00602.
OMA	DLLFAWK.
ProtClustDB	PRK00881.
Enzyme and pathway databases
UniPathway	UPA00074; UER00133. UPA00074; UER00135.
Family and domain databases
Gene3D	3.40.140.20. 2 hits. 3.40.50.1380. 1 hit.
HAMAP	MF_00139. PurH.
InterPro	IPR024051. AICAR_Tfase_dom. IPR002695. AICARFT_IMPCHas. IPR016193. Cytidine_deaminase-like. IPR011607. MGS-like_dom. [Graphical view]
PANTHER	PTHR11692. PTHR11692. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit. SSF52335. MGS-like_dom. 1 hit.
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_VIBCH

Accession

Primary (citable) accession number: Q9KV80

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program