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Protein

UDP-N-acetylenolpyruvoylglucosamine reductase

Gene

murB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

UDP-N-acetyl-alpha-D-muramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH.UniRule annotation

Cofactori

FADUniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei163 – 1631UniRule annotation
Active sitei232 – 2321Proton donorUniRule annotation
Active sitei327 – 3271UniRule annotation

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: InterPro
  • UDP-N-acetylmuramate dehydrogenase activity Source: TIGR

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: TIGR
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductaseUniRule annotation (EC:1.3.1.98UniRule annotation)
Alternative name(s):
UDP-N-acetylmuramate dehydrogenaseUniRule annotation
Gene namesi
Name:murBUniRule annotation
Ordered Locus Names:VC_0318
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347UDP-N-acetylenolpyruvoylglucosamine reductasePRO_0000179285Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243277.VC0318.

Structurei

Secondary structure

1
347
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96Combined sources
Helixi10 – 123Combined sources
Beta strandi20 – 278Combined sources
Helixi30 – 378Combined sources
Beta strandi38 – 403Combined sources
Turni41 – 444Combined sources
Beta strandi47 – 526Combined sources
Beta strandi56 – 583Combined sources
Beta strandi62 – 698Combined sources
Beta strandi74 – 785Combined sources
Beta strandi80 – 889Combined sources
Helixi93 – 10210Combined sources
Helixi109 – 1113Combined sources
Helixi118 – 1203Combined sources
Turni121 – 1255Combined sources
Helixi133 – 1353Combined sources
Beta strandi137 – 1448Combined sources
Turni145 – 1473Combined sources
Beta strandi148 – 1547Combined sources
Helixi156 – 1583Combined sources
Helixi166 – 1683Combined sources
Turni169 – 1746Combined sources
Beta strandi175 – 18713Combined sources
Helixi195 – 1995Combined sources
Helixi206 – 22015Combined sources
Turni224 – 2263Combined sources
Beta strandi229 – 2346Combined sources
Helixi241 – 25010Combined sources
Beta strandi260 – 2656Combined sources
Helixi267 – 2737Combined sources
Beta strandi284 – 2863Combined sources
Beta strandi293 – 2964Combined sources
Helixi302 – 32019Combined sources
Beta strandi328 – 3314Combined sources
Beta strandi333 – 3364Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3I99X-ray2.20A1-347[»]
ProteinModelPortaliQ9KV40.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KV40.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 187171FAD-binding PCMH-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MurB family.UniRule annotation
Contains 1 FAD-binding PCMH-type domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D4A. Bacteria.
COG0812. LUCA.
KOiK00075.
OMAiMQNIGAY.
OrthoDBiEOG60CWQ3.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.78.10. 1 hit.
HAMAPiMF_00037. MurB.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR003170. MurB.
IPR011601. MurB_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR21071. PTHR21071. 1 hit.
PfamiPF01565. FAD_binding_4. 1 hit.
PF02873. MurB_C. 1 hit.
[Graphical view]
SUPFAMiSSF56176. SSF56176. 1 hit.
SSF56194. SSF56194. 1 hit.
TIGRFAMsiTIGR00179. murB. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KV40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIQLGANLK PYHTFGIEQL AAQLVVAESI DDLKALYCSA EWASLPKLII
60 70 80 90 100
GKGSNMLFTC HYTGMIVVNR LNGIEHQQDD DYHRLHVAGG EDWPSLVSWC
110 120 130 140 150
VEQGIGGLEN LALIPGCAGS APIQNIGAYG VEFKDVCDYV EYLCLETGTV
160 170 180 190 200
KRLTMEECQF GYRDSIFKHQ LYQKAVVTAV GLKFAKAWQP IIQYGPLKDL
210 220 230 240 250
SSDCAIHDVY QRVCATRMEK LPDPAVMGNA GSFFKNPVIS QQAFARLQIE
260 270 280 290 300
HPDVVAYPAE QGVKVAAGWL IDQAGLKGHQ IGGAKVHPKQ ALVIVNTGDA
310 320 330 340
SAQDVLMLAA DIQQRVFNCY GIELEHEVRF IGESEETNLK QWMSEQA
Length:347
Mass (Da):38,271
Last modified:April 30, 2003 - v2
Checksum:iC4758B7E14A31CD6
GO

Sequence cautioni

The sequence AAF93491.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93491.1. Different initiation.
PIRiD82337.
RefSeqiNP_229972.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93491; AAF93491; VC_0318.
GeneIDi2615109.
KEGGivch:VC0318.
PATRICi20079685. VBIVibCho83274_0295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93491.1. Different initiation.
PIRiD82337.
RefSeqiNP_229972.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3I99X-ray2.20A1-347[»]
ProteinModelPortaliQ9KV40.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0318.

Protocols and materials databases

DNASUi2615109.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93491; AAF93491; VC_0318.
GeneIDi2615109.
KEGGivch:VC0318.
PATRICi20079685. VBIVibCho83274_0295.

Phylogenomic databases

eggNOGiENOG4105D4A. Bacteria.
COG0812. LUCA.
KOiK00075.
OMAiMQNIGAY.
OrthoDBiEOG60CWQ3.

Enzyme and pathway databases

UniPathwayiUPA00219.

Miscellaneous databases

EvolutionaryTraceiQ9KV40.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.78.10. 1 hit.
HAMAPiMF_00037. MurB.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR003170. MurB.
IPR011601. MurB_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR21071. PTHR21071. 1 hit.
PfamiPF01565. FAD_binding_4. 1 hit.
PF02873. MurB_C. 1 hit.
[Graphical view]
SUPFAMiSSF56176. SSF56176. 1 hit.
SSF56194. SSF56194. 1 hit.
TIGRFAMsiTIGR00179. murB. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiMURB_VIBCH
AccessioniPrimary (citable) accession number: Q9KV40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: April 30, 2003
Last modified: November 11, 2015
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.