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Protein

UDP-N-acetylenolpyruvoylglucosamine reductase

Gene

murB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

UDP-N-acetyl-alpha-D-muramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH.UniRule annotation

Cofactori

FADUniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei163UniRule annotation1
Active sitei232Proton donorUniRule annotation1
Active sitei327UniRule annotation1

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: InterPro
  • UDP-N-acetylmuramate dehydrogenase activity Source: TIGR

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: TIGR
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductaseUniRule annotation (EC:1.3.1.98UniRule annotation)
Alternative name(s):
UDP-N-acetylmuramate dehydrogenaseUniRule annotation
Gene namesi
Name:murBUniRule annotation
Ordered Locus Names:VC_0318
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001792851 – 347UDP-N-acetylenolpyruvoylglucosamine reductaseAdd BLAST347

Interactioni

Protein-protein interaction databases

STRINGi243277.VC0318.

Structurei

Secondary structure

1347
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Helixi10 – 12Combined sources3
Beta strandi20 – 27Combined sources8
Helixi30 – 37Combined sources8
Beta strandi38 – 40Combined sources3
Turni41 – 44Combined sources4
Beta strandi47 – 52Combined sources6
Beta strandi56 – 58Combined sources3
Beta strandi62 – 69Combined sources8
Beta strandi74 – 78Combined sources5
Beta strandi80 – 88Combined sources9
Helixi93 – 102Combined sources10
Helixi109 – 111Combined sources3
Helixi118 – 120Combined sources3
Turni121 – 125Combined sources5
Helixi133 – 135Combined sources3
Beta strandi137 – 144Combined sources8
Turni145 – 147Combined sources3
Beta strandi148 – 154Combined sources7
Helixi156 – 158Combined sources3
Helixi166 – 168Combined sources3
Turni169 – 174Combined sources6
Beta strandi175 – 187Combined sources13
Helixi195 – 199Combined sources5
Helixi206 – 220Combined sources15
Turni224 – 226Combined sources3
Beta strandi229 – 234Combined sources6
Helixi241 – 250Combined sources10
Beta strandi260 – 265Combined sources6
Helixi267 – 273Combined sources7
Beta strandi284 – 286Combined sources3
Beta strandi293 – 296Combined sources4
Helixi302 – 320Combined sources19
Beta strandi328 – 331Combined sources4
Beta strandi333 – 336Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I99X-ray2.20A1-347[»]
ProteinModelPortaliQ9KV40.
SMRiQ9KV40.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KV40.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 187FAD-binding PCMH-typeUniRule annotationAdd BLAST171

Sequence similaritiesi

Belongs to the MurB family.UniRule annotation
Contains 1 FAD-binding PCMH-type domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D4A. Bacteria.
COG0812. LUCA.
KOiK00075.
OMAiMQNIGAY.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.78.10. 1 hit.
HAMAPiMF_00037. MurB. 1 hit.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR003170. MurB.
IPR011601. MurB_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR21071. PTHR21071. 1 hit.
PfamiPF01565. FAD_binding_4. 1 hit.
PF02873. MurB_C. 1 hit.
[Graphical view]
SUPFAMiSSF56176. SSF56176. 1 hit.
SSF56194. SSF56194. 1 hit.
TIGRFAMsiTIGR00179. murB. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KV40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIQLGANLK PYHTFGIEQL AAQLVVAESI DDLKALYCSA EWASLPKLII
60 70 80 90 100
GKGSNMLFTC HYTGMIVVNR LNGIEHQQDD DYHRLHVAGG EDWPSLVSWC
110 120 130 140 150
VEQGIGGLEN LALIPGCAGS APIQNIGAYG VEFKDVCDYV EYLCLETGTV
160 170 180 190 200
KRLTMEECQF GYRDSIFKHQ LYQKAVVTAV GLKFAKAWQP IIQYGPLKDL
210 220 230 240 250
SSDCAIHDVY QRVCATRMEK LPDPAVMGNA GSFFKNPVIS QQAFARLQIE
260 270 280 290 300
HPDVVAYPAE QGVKVAAGWL IDQAGLKGHQ IGGAKVHPKQ ALVIVNTGDA
310 320 330 340
SAQDVLMLAA DIQQRVFNCY GIELEHEVRF IGESEETNLK QWMSEQA
Length:347
Mass (Da):38,271
Last modified:April 30, 2003 - v2
Checksum:iC4758B7E14A31CD6
GO

Sequence cautioni

The sequence AAF93491 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93491.1. Different initiation.
PIRiD82337.
RefSeqiNP_229972.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93491; AAF93491; VC_0318.
GeneIDi2615109.
KEGGivch:VC0318.
PATRICi20079685. VBIVibCho83274_0295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93491.1. Different initiation.
PIRiD82337.
RefSeqiNP_229972.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I99X-ray2.20A1-347[»]
ProteinModelPortaliQ9KV40.
SMRiQ9KV40.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0318.

Protocols and materials databases

DNASUi2615109.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93491; AAF93491; VC_0318.
GeneIDi2615109.
KEGGivch:VC0318.
PATRICi20079685. VBIVibCho83274_0295.

Phylogenomic databases

eggNOGiENOG4105D4A. Bacteria.
COG0812. LUCA.
KOiK00075.
OMAiMQNIGAY.

Enzyme and pathway databases

UniPathwayiUPA00219.

Miscellaneous databases

EvolutionaryTraceiQ9KV40.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.78.10. 1 hit.
HAMAPiMF_00037. MurB. 1 hit.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR003170. MurB.
IPR011601. MurB_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR21071. PTHR21071. 1 hit.
PfamiPF01565. FAD_binding_4. 1 hit.
PF02873. MurB_C. 1 hit.
[Graphical view]
SUPFAMiSSF56176. SSF56176. 1 hit.
SSF56194. SSF56194. 1 hit.
TIGRFAMsiTIGR00179. murB. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMURB_VIBCH
AccessioniPrimary (citable) accession number: Q9KV40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: April 30, 2003
Last modified: November 2, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.