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Q9KV30

- RPOB_VIBCH

UniProt

Q9KV30 - RPOB_VIBCH

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Protein

DNA-directed RNA polymerase subunit beta

Gene

rpoB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. DNA-directed RNA polymerase activity Source: TIGR
  3. ribonucleoside binding Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit betaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit betaUniRule annotation
Alternative name(s):
RNA polymerase subunit betaUniRule annotation
Transcriptase subunit betaUniRule annotation
Gene namesi
Name:rpoBUniRule annotation
Ordered Locus Names:VC_0328
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584: Chromosome 1

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13411341DNA-directed RNA polymerase subunit betaPRO_0000047992Add
BLAST

Proteomic databases

PRIDEiQ9KV30.

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

Protein-protein interaction databases

STRINGi243277.VC0328.

Structurei

Secondary structure

1
1341
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi228 – 24114
Helixi243 – 2464
Beta strandi255 – 2573
Beta strandi260 – 2634
Helixi271 – 2799
Beta strandi284 – 2874
Helixi289 – 2935
Beta strandi308 – 31710
Helixi319 – 3279

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3E7HX-ray1.70A/B228-329[»]
ProteinModelPortaliQ9KV30.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KV30.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0085.
KOiK03043.
OMAiICPANME.
OrthoDBiEOG6M9DS6.

Family and domain databases

Gene3Di2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 2 hits.
3.90.1110.10. 2 hits.
HAMAPiMF_01321. RNApol_bact_RpoB.
InterProiIPR010243. DNA-dir_RNA_pol_bsu.
IPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 2 hits.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02013. rpoB. 1 hit.
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KV30-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVYSYTEKKR IRKDFGTRPQ VLDIPYLLSI QLDSFEKFIE QDPEGQYGLE
60 70 80 90 100
AAFRSVFPIQ SYNGNSELQY VSYRLGEPVF DVKECQIRGV TYSKPLRVKL
110 120 130 140 150
RLVIFDKDAP AGTVKDIKEQ EVYMGEIPLM TENGTFVING TERVIVSQLH
160 170 180 190 200
RSPGVFFDSD KGKTHSSGKV LYNARIIPYR GSWLDFEFDP KDNLYVRIDR
210 220 230 240 250
RRKLPASIIL RALGKTSAEI LDIFFEKVNF EVKDQTLMME LVPERLRGET
260 270 280 290 300
ATFDIEADGK VYVEKGRRVT ARHIRQLEKD GVNFIEVPVE YIVGKVSAKD
310 320 330 340 350
YVNEATGELI ITANQEISLE ALANLSQAGY KKLEVLFTND LDHGPFMSET
360 370 380 390 400
LRVDSTTDRI SALVEIYRMM RPGEPPTKEA AESLFESLFF SAERYDLSTV
410 420 430 440 450
GRMKFNSSIG REDAEEQGTL DEVDIIEVMK KLISIRNGKG EVDDIDHLGN
460 470 480 490 500
RRIRSVGEMA ENQFRVGLVR VERAVKERLS LGDLDNVMPQ DLINAKPISA
510 520 530 540 550
AVKEFFGSSQ LSQFMDQNNP LSEVTHKRRI SALGPGGLTR ERAGFEVRDV
560 570 580 590 600
HVTHYGRLCP IETPEGPNIG LINSLSAFAR CNEYGFLETP YRRVVNGVVT
610 620 630 640 650
DEVDYLSAIE EGQFVIAQAN AKLTEEGGFA DELVTARQKG ESGLHPREHV
660 670 680 690 700
DYMDVATNQV VSIAASLIPF LEHDDANRAL MGANMQRQAV PTLRSEKPLV
710 720 730 740 750
GTGIERNVAV DSGVTAVAKR GGVIQSVDAS RIVVKVNEEE LIPGEAGIDI
760 770 780 790 800
YNLTKYTRSN QNTCINQRPC VMPGEPVARG DVLADGPSTD LGELALGQNM
810 820 830 840 850
RIAFMPWNGY NFEDSILVSE RVVQDDRFTT IHIQELSCVA RDTKLGAEEI
860 870 880 890 900
TADIPNVGEA ALSKLDESGI VYIGAEVKGG DILVGKVTPK GETQLTPEEK
910 920 930 940 950
LLRAIFGEKA SDVKDTSLRV PNSVAGTVID VQVFTRDGVE KDKRALEIEQ
960 970 980 990 1000
MQLKEAKKDL TEEFQILEGG LLARVRSVLL AGGYTEAKLG SIERKKWLEQ
1010 1020 1030 1040 1050
TLENEELQNQ LEQLAEQYDE LKADFDKKFE AKRRKITQGD DLAPGVLKIV
1060 1070 1080 1090 1100
KVYLAVKRRI QPGDKMAGRH GNKGVISKIN PVEDMPYDEN GQPVDIVLNP
1110 1120 1130 1140 1150
LGVPSRMNIG QILEVHLGLA AKGIGDKINQ MIKEQQELAK LREFLQKVYD
1160 1170 1180 1190 1200
LGDTRQRVDI SELSDEDVRT LAHNLRAGLP VATPVFDGAP ESSIKAMLEL
1210 1220 1230 1240 1250
ADLPASGQLT LFDGRTGDAF ERPVTVGYMY MLKLNHLVDD KMHARSTGSY
1260 1270 1280 1290 1300
SLVTQQPLGG KAQFGGQRFG EMEVWALEAY GAAYTLQEML TVKSDDVNGR
1310 1320 1330 1340
TKMYKNIVDG NHAMEPGMPE SFNVLLKEIR SLGINIELED E
Length:1,341
Mass (Da):149,455
Last modified:January 16, 2004 - v2
Checksum:i9C6C824A923295A9
GO

Sequence cautioni

The sequence AAF93501.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE003852 Genomic DNA. Translation: AAF93501.1. Different initiation.
PIRiF82336.
RefSeqiNP_229982.2. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93501; AAF93501; VC_0328.
GeneIDi2615094.
KEGGivch:VC0328.
PATRICi20079713. VBIVibCho83274_0305.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE003852 Genomic DNA. Translation: AAF93501.1 . Different initiation.
PIRi F82336.
RefSeqi NP_229982.2. NC_002505.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3E7H X-ray 1.70 A/B 228-329 [» ]
ProteinModelPortali Q9KV30.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243277.VC0328.

Proteomic databases

PRIDEi Q9KV30.

Protocols and materials databases

DNASUi 2615094.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF93501 ; AAF93501 ; VC_0328 .
GeneIDi 2615094.
KEGGi vch:VC0328.
PATRICi 20079713. VBIVibCho83274_0305.

Phylogenomic databases

eggNOGi COG0085.
KOi K03043.
OMAi ICPANME.
OrthoDBi EOG6M9DS6.

Miscellaneous databases

EvolutionaryTracei Q9KV30.

Family and domain databases

Gene3Di 2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 2 hits.
3.90.1110.10. 2 hits.
HAMAPi MF_01321. RNApol_bact_RpoB.
InterProi IPR010243. DNA-dir_RNA_pol_bsu.
IPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view ]
PANTHERi PTHR20856. PTHR20856. 1 hit.
Pfami PF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 2 hits.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02013. rpoB. 1 hit.
PROSITEi PS01166. RNA_POL_BETA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiRPOB_VIBCH
AccessioniPrimary (citable) accession number: Q9KV30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: January 16, 2004
Last modified: October 1, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3