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Q9KV30 (RPOB_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit beta

Short name=RNAP subunit beta
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta
Gene names
Name:rpoB
Ordered Locus Names:VC_0328
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length1341 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP-Rule MF_01321

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_01321

Subunit structure

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.

Sequence similarities

Belongs to the RNA polymerase beta chain family.

Sequence caution

The sequence AAF93501.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13411341DNA-directed RNA polymerase subunit beta HAMAP-Rule MF_01321
PRO_0000047992

Secondary structure

................... 1341
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9KV30 [UniParc].

Last modified January 16, 2004. Version 2.
Checksum: 9C6C824A923295A9

FASTA1,341149,455
        10         20         30         40         50         60 
MVYSYTEKKR IRKDFGTRPQ VLDIPYLLSI QLDSFEKFIE QDPEGQYGLE AAFRSVFPIQ 

        70         80         90        100        110        120 
SYNGNSELQY VSYRLGEPVF DVKECQIRGV TYSKPLRVKL RLVIFDKDAP AGTVKDIKEQ 

       130        140        150        160        170        180 
EVYMGEIPLM TENGTFVING TERVIVSQLH RSPGVFFDSD KGKTHSSGKV LYNARIIPYR 

       190        200        210        220        230        240 
GSWLDFEFDP KDNLYVRIDR RRKLPASIIL RALGKTSAEI LDIFFEKVNF EVKDQTLMME 

       250        260        270        280        290        300 
LVPERLRGET ATFDIEADGK VYVEKGRRVT ARHIRQLEKD GVNFIEVPVE YIVGKVSAKD 

       310        320        330        340        350        360 
YVNEATGELI ITANQEISLE ALANLSQAGY KKLEVLFTND LDHGPFMSET LRVDSTTDRI 

       370        380        390        400        410        420 
SALVEIYRMM RPGEPPTKEA AESLFESLFF SAERYDLSTV GRMKFNSSIG REDAEEQGTL 

       430        440        450        460        470        480 
DEVDIIEVMK KLISIRNGKG EVDDIDHLGN RRIRSVGEMA ENQFRVGLVR VERAVKERLS 

       490        500        510        520        530        540 
LGDLDNVMPQ DLINAKPISA AVKEFFGSSQ LSQFMDQNNP LSEVTHKRRI SALGPGGLTR 

       550        560        570        580        590        600 
ERAGFEVRDV HVTHYGRLCP IETPEGPNIG LINSLSAFAR CNEYGFLETP YRRVVNGVVT 

       610        620        630        640        650        660 
DEVDYLSAIE EGQFVIAQAN AKLTEEGGFA DELVTARQKG ESGLHPREHV DYMDVATNQV 

       670        680        690        700        710        720 
VSIAASLIPF LEHDDANRAL MGANMQRQAV PTLRSEKPLV GTGIERNVAV DSGVTAVAKR 

       730        740        750        760        770        780 
GGVIQSVDAS RIVVKVNEEE LIPGEAGIDI YNLTKYTRSN QNTCINQRPC VMPGEPVARG 

       790        800        810        820        830        840 
DVLADGPSTD LGELALGQNM RIAFMPWNGY NFEDSILVSE RVVQDDRFTT IHIQELSCVA 

       850        860        870        880        890        900 
RDTKLGAEEI TADIPNVGEA ALSKLDESGI VYIGAEVKGG DILVGKVTPK GETQLTPEEK 

       910        920        930        940        950        960 
LLRAIFGEKA SDVKDTSLRV PNSVAGTVID VQVFTRDGVE KDKRALEIEQ MQLKEAKKDL 

       970        980        990       1000       1010       1020 
TEEFQILEGG LLARVRSVLL AGGYTEAKLG SIERKKWLEQ TLENEELQNQ LEQLAEQYDE 

      1030       1040       1050       1060       1070       1080 
LKADFDKKFE AKRRKITQGD DLAPGVLKIV KVYLAVKRRI QPGDKMAGRH GNKGVISKIN 

      1090       1100       1110       1120       1130       1140 
PVEDMPYDEN GQPVDIVLNP LGVPSRMNIG QILEVHLGLA AKGIGDKINQ MIKEQQELAK 

      1150       1160       1170       1180       1190       1200 
LREFLQKVYD LGDTRQRVDI SELSDEDVRT LAHNLRAGLP VATPVFDGAP ESSIKAMLEL 

      1210       1220       1230       1240       1250       1260 
ADLPASGQLT LFDGRTGDAF ERPVTVGYMY MLKLNHLVDD KMHARSTGSY SLVTQQPLGG 

      1270       1280       1290       1300       1310       1320 
KAQFGGQRFG EMEVWALEAY GAAYTLQEML TVKSDDVNGR TKMYKNIVDG NHAMEPGMPE 

      1330       1340 
SFNVLLKEIR SLGINIELED E 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE003852 Genomic DNA. Translation: AAF93501.1. Different initiation.
PIRF82336.
RefSeqNP_229982.2. NC_002505.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3E7HX-ray1.70A/B228-329[»]
ProteinModelPortalQ9KV30.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243277.VC0328.

Proteomic databases

PRIDEQ9KV30.

Protocols and materials databases

DNASU2615094.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF93501; AAF93501; VC_0328.
GeneID2615094.
KEGGvch:VC0328.
PATRIC20079713. VBIVibCho83274_0305.

Phylogenomic databases

eggNOGCOG0085.
KOK03043.
OMAICPANME.
OrthoDBEOG6M9DS6.

Family and domain databases

Gene3D2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 2 hits.
3.90.1110.10. 2 hits.
HAMAPMF_01321. RNApol_bact_RpoB.
InterProIPR010243. DNA-dir_RNA_pol_bsu.
IPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERPTHR20856. PTHR20856. 1 hit.
PfamPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 2 hits.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsTIGR02013. rpoB. 1 hit.
PROSITEPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9KV30.

Entry information

Entry nameRPOB_VIBCH
AccessionPrimary (citable) accession number: Q9KV30
Entry history
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: January 16, 2004
Last modified: June 11, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references