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Q9KV30

- RPOB_VIBCH

UniProt

Q9KV30 - RPOB_VIBCH

Protein

DNA-directed RNA polymerase subunit beta

Gene

rpoB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (16 Jan 2004)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-HAMAP
    2. DNA-directed RNA polymerase activity Source: TIGR
    3. ribonucleoside binding Source: InterPro

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Transcription

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase subunit betaUniRule annotation (EC:2.7.7.6UniRule annotation)
    Short name:
    RNAP subunit betaUniRule annotation
    Alternative name(s):
    RNA polymerase subunit betaUniRule annotation
    Transcriptase subunit betaUniRule annotation
    Gene namesi
    Name:rpoBUniRule annotation
    Ordered Locus Names:VC_0328
    OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
    Taxonomic identifieri243277 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000000584: Chromosome 1

    Subcellular locationi

    Keywords - Cellular componenti

    DNA-directed RNA polymerase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13411341DNA-directed RNA polymerase subunit betaPRO_0000047992Add
    BLAST

    Proteomic databases

    PRIDEiQ9KV30.

    Interactioni

    Subunit structurei

    The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

    Protein-protein interaction databases

    STRINGi243277.VC0328.

    Structurei

    Secondary structure

    1
    1341
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi228 – 24114
    Helixi243 – 2464
    Beta strandi255 – 2573
    Beta strandi260 – 2634
    Helixi271 – 2799
    Beta strandi284 – 2874
    Helixi289 – 2935
    Beta strandi308 – 31710
    Helixi319 – 3279

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3E7HX-ray1.70A/B228-329[»]
    ProteinModelPortaliQ9KV30.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9KV30.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNA polymerase beta chain family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0085.
    KOiK03043.
    OMAiICPANME.
    OrthoDBiEOG6M9DS6.

    Family and domain databases

    Gene3Di2.30.150.10. 1 hit.
    2.40.270.10. 2 hits.
    2.40.50.150. 2 hits.
    3.90.1110.10. 2 hits.
    HAMAPiMF_01321. RNApol_bact_RpoB.
    InterProiIPR010243. DNA-dir_RNA_pol_bsu.
    IPR019462. DNA-dir_RNA_pol_bsu_external_1.
    IPR015712. DNA-dir_RNA_pol_su2.
    IPR007120. DNA-dir_RNA_pol_su2_6.
    IPR007121. RNA_pol_bsu_CS.
    IPR007644. RNA_pol_bsu_protrusion.
    IPR007642. RNA_pol_Rpb2_2.
    IPR007645. RNA_pol_Rpb2_3.
    IPR007641. RNA_pol_Rpb2_7.
    IPR014724. RNA_pol_RPB2_OB-fold.
    [Graphical view]
    PANTHERiPTHR20856. PTHR20856. 1 hit.
    PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
    PF04561. RNA_pol_Rpb2_2. 2 hits.
    PF04565. RNA_pol_Rpb2_3. 1 hit.
    PF10385. RNA_pol_Rpb2_45. 1 hit.
    PF00562. RNA_pol_Rpb2_6. 1 hit.
    PF04560. RNA_pol_Rpb2_7. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02013. rpoB. 1 hit.
    PROSITEiPS01166. RNA_POL_BETA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9KV30-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVYSYTEKKR IRKDFGTRPQ VLDIPYLLSI QLDSFEKFIE QDPEGQYGLE     50
    AAFRSVFPIQ SYNGNSELQY VSYRLGEPVF DVKECQIRGV TYSKPLRVKL 100
    RLVIFDKDAP AGTVKDIKEQ EVYMGEIPLM TENGTFVING TERVIVSQLH 150
    RSPGVFFDSD KGKTHSSGKV LYNARIIPYR GSWLDFEFDP KDNLYVRIDR 200
    RRKLPASIIL RALGKTSAEI LDIFFEKVNF EVKDQTLMME LVPERLRGET 250
    ATFDIEADGK VYVEKGRRVT ARHIRQLEKD GVNFIEVPVE YIVGKVSAKD 300
    YVNEATGELI ITANQEISLE ALANLSQAGY KKLEVLFTND LDHGPFMSET 350
    LRVDSTTDRI SALVEIYRMM RPGEPPTKEA AESLFESLFF SAERYDLSTV 400
    GRMKFNSSIG REDAEEQGTL DEVDIIEVMK KLISIRNGKG EVDDIDHLGN 450
    RRIRSVGEMA ENQFRVGLVR VERAVKERLS LGDLDNVMPQ DLINAKPISA 500
    AVKEFFGSSQ LSQFMDQNNP LSEVTHKRRI SALGPGGLTR ERAGFEVRDV 550
    HVTHYGRLCP IETPEGPNIG LINSLSAFAR CNEYGFLETP YRRVVNGVVT 600
    DEVDYLSAIE EGQFVIAQAN AKLTEEGGFA DELVTARQKG ESGLHPREHV 650
    DYMDVATNQV VSIAASLIPF LEHDDANRAL MGANMQRQAV PTLRSEKPLV 700
    GTGIERNVAV DSGVTAVAKR GGVIQSVDAS RIVVKVNEEE LIPGEAGIDI 750
    YNLTKYTRSN QNTCINQRPC VMPGEPVARG DVLADGPSTD LGELALGQNM 800
    RIAFMPWNGY NFEDSILVSE RVVQDDRFTT IHIQELSCVA RDTKLGAEEI 850
    TADIPNVGEA ALSKLDESGI VYIGAEVKGG DILVGKVTPK GETQLTPEEK 900
    LLRAIFGEKA SDVKDTSLRV PNSVAGTVID VQVFTRDGVE KDKRALEIEQ 950
    MQLKEAKKDL TEEFQILEGG LLARVRSVLL AGGYTEAKLG SIERKKWLEQ 1000
    TLENEELQNQ LEQLAEQYDE LKADFDKKFE AKRRKITQGD DLAPGVLKIV 1050
    KVYLAVKRRI QPGDKMAGRH GNKGVISKIN PVEDMPYDEN GQPVDIVLNP 1100
    LGVPSRMNIG QILEVHLGLA AKGIGDKINQ MIKEQQELAK LREFLQKVYD 1150
    LGDTRQRVDI SELSDEDVRT LAHNLRAGLP VATPVFDGAP ESSIKAMLEL 1200
    ADLPASGQLT LFDGRTGDAF ERPVTVGYMY MLKLNHLVDD KMHARSTGSY 1250
    SLVTQQPLGG KAQFGGQRFG EMEVWALEAY GAAYTLQEML TVKSDDVNGR 1300
    TKMYKNIVDG NHAMEPGMPE SFNVLLKEIR SLGINIELED E 1341
    Length:1,341
    Mass (Da):149,455
    Last modified:January 16, 2004 - v2
    Checksum:i9C6C824A923295A9
    GO

    Sequence cautioni

    The sequence AAF93501.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE003852 Genomic DNA. Translation: AAF93501.1. Different initiation.
    PIRiF82336.
    RefSeqiNP_229982.2. NC_002505.1.

    Genome annotation databases

    EnsemblBacteriaiAAF93501; AAF93501; VC_0328.
    GeneIDi2615094.
    KEGGivch:VC0328.
    PATRICi20079713. VBIVibCho83274_0305.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE003852 Genomic DNA. Translation: AAF93501.1 . Different initiation.
    PIRi F82336.
    RefSeqi NP_229982.2. NC_002505.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3E7H X-ray 1.70 A/B 228-329 [» ]
    ProteinModelPortali Q9KV30.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243277.VC0328.

    Proteomic databases

    PRIDEi Q9KV30.

    Protocols and materials databases

    DNASUi 2615094.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF93501 ; AAF93501 ; VC_0328 .
    GeneIDi 2615094.
    KEGGi vch:VC0328.
    PATRICi 20079713. VBIVibCho83274_0305.

    Phylogenomic databases

    eggNOGi COG0085.
    KOi K03043.
    OMAi ICPANME.
    OrthoDBi EOG6M9DS6.

    Miscellaneous databases

    EvolutionaryTracei Q9KV30.

    Family and domain databases

    Gene3Di 2.30.150.10. 1 hit.
    2.40.270.10. 2 hits.
    2.40.50.150. 2 hits.
    3.90.1110.10. 2 hits.
    HAMAPi MF_01321. RNApol_bact_RpoB.
    InterProi IPR010243. DNA-dir_RNA_pol_bsu.
    IPR019462. DNA-dir_RNA_pol_bsu_external_1.
    IPR015712. DNA-dir_RNA_pol_su2.
    IPR007120. DNA-dir_RNA_pol_su2_6.
    IPR007121. RNA_pol_bsu_CS.
    IPR007644. RNA_pol_bsu_protrusion.
    IPR007642. RNA_pol_Rpb2_2.
    IPR007645. RNA_pol_Rpb2_3.
    IPR007641. RNA_pol_Rpb2_7.
    IPR014724. RNA_pol_RPB2_OB-fold.
    [Graphical view ]
    PANTHERi PTHR20856. PTHR20856. 1 hit.
    Pfami PF04563. RNA_pol_Rpb2_1. 1 hit.
    PF04561. RNA_pol_Rpb2_2. 2 hits.
    PF04565. RNA_pol_Rpb2_3. 1 hit.
    PF10385. RNA_pol_Rpb2_45. 1 hit.
    PF00562. RNA_pol_Rpb2_6. 1 hit.
    PF04560. RNA_pol_Rpb2_7. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02013. rpoB. 1 hit.
    PROSITEi PS01166. RNA_POL_BETA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39315 / El Tor Inaba N16961.

    Entry informationi

    Entry nameiRPOB_VIBCH
    AccessioniPrimary (citable) accession number: Q9KV30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2001
    Last sequence update: January 16, 2004
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3