Q9KV29 (RPOC_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta'
Short name=RNAP subunit beta'
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'

Gene names

Name:	rpoC
Ordered Locus Names:	VC_0329

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	1401 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01322
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01322
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1401	1401	DNA-directed RNA polymerase subunit beta' HAMAP MF_01322		PRO_0000067827

Sequences

	Sequence		Length	Mass (Da)
	Q9KV29 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: DFDB0F2B5514504F	FASTA	1,401	155,023
	10 20 30 40 50 60 MKDLLNFLKA QHKTEEFDAI KIGLASPDMI RSWSFGEVKK PETINYRTFK PERDGLFCAR 70 80 90 100 110 120 IFGPVKDYEC LCGKYKRLKH RGVICEKCGV EVTQTKVRRD RMGHIELASP VAHIWFLKSL 130 140 150 160 170 180 PSRIGLLMDM PLRDIERVLY FEMYVVTEPG MTDLERGQML TEEEYLDRLE EWGDEFTAKM 190 200 210 220 230 240 GAEAIKDLLA SMDLPAEAEQ MREELDTTNS ETKRKKLTKR LKLVEAFVAS GNKPEWMILT 250 260 270 280 290 300 VLPVLPPDLR PLVPLDGGRF ATSDLNDLYR RVINRNNRLK RLLELAAPDI IVRNEKRMLQ 310 320 330 340 350 360 ESVDALLDNG RRGRAITGSN KRPLKSLADM IKGKQGRFRQ NLLGKRVDYS GRSVITVGPY 370 380 390 400 410 420 LRLHQCGLPK KMALELFKPF IYSKLETRGL ATTIKAAKKM VEREEAVVWD ILDEVIREHP 430 440 450 460 470 480 VLLNRAPTLH RLGIQAFEPV LIEGKAIQLH PLVCAAYNAD FDGDQMAVHV PLTLEAQLEA 490 500 510 520 530 540 RTLMMSTNNI LSPASGDPII VPSQDVVLGL YYMTREKINA KGEGMYLTGP AEAEKAYRTK 550 560 570 580 590 600 TAELHARVKV RITETIKHEN GKLTTETKMI DTTVGRAMLW QIVPKGLPYS LVNQKLGKKQ 610 620 630 640 650 660 ISNLLNEAYR KLGLKDTVIF ADQIMYTGFA YAALSGVSVG IDDMVVPAAK YTEIAEAEEE 670 680 690 700 710 720 VREIQEQFQS GLVTAGERYN KVIDIWASTN DRVAKAMMEN LSSEQVINRQ GEQEKQESFN 730 740 750 760 770 780 SIYMMADSGA RGSAAQIRQL AGMRGLMARP DGSIIETPIT ANFKEGLNVL QYFISTHGAR 790 800 810 820 830 840 KGLADTALKT ANSGYLTRRL VDVAQDVVVT EHDCGTLEGV VMTPHIEGGD VKVALTELAL 850 860 870 880 890 900 GRVVSEDILK PGTDEVLIPR NTLLDEKWCK VINDNSVDQI KVRSVVTCDS DFGCCAQCYG 910 920 930 940 950 960 RDLARGHLVN QGEAVGVIAA QSIGEPGTQL TMRTFHIGGA ASTAAAENSI QAKNNGSVKL 970 980 990 1000 1010 1020 HNAKFVTNKD GKLVITSRAS ELTIIDEFGR TKEKHKLPYG SMLSKADGDA VAAGETVANW 1030 1040 1050 1060 1070 1080 EAHTMPIITE VAGRVQFVDM IDGVTVSRQT DDLTGLSSSE VTEAAARPAA GKDMRPAIKL 1090 1100 1110 1120 1130 1140 VDANGKDVLI PGTDMPAQYF LPGKAIVNLD DGAEVNVGDT LARIPQKSGG NKDITGGLPR 1150 1160 1170 1180 1190 1200 VADLFEARKP KEPAILAEHS GTVSFGKETK GKRRLIITRD SGDTYEEMIP KHRQLNVFEG 1210 1220 1230 1240 1250 1260 ERIERGDVIA DGPESPHDIL RLRGIHAVTT YIANEVQEVY RLQGVKINDK HIETIVRQML 1270 1280 1290 1300 1310 1320 RKCTITFAGD SEFLPGETVE YSQVKIANRK LVEEGKEPAR FERELLGITK ASLATESFIS 1330 1340 1350 1360 1370 1380 AASFQETTRV LTEAAVSGKR DDLRGLKENV IVGRLIPAGT GFAYHQDRQA KRAQEQQGPS 1390 1400 AEQATDNLAA LLNAGFSSDD E

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF93502.1.
PIR	G82336.
RefSeq	NP_229983.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KV29.
SMR	Q9KV29. Positions 15-165, 908-942, 944-1128.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2615095.
GenomeReviews	Gene locus VC_0329 in contig AE003852_GR.
KEGG	vch:VC0329.
PATRIC	20079715. VBIVibCho83274_0306.
TIGR	VC_0329.
Phylogenomic databases
HOGENOM	HBG621785.
OMA	DINDKHI.
PhylomeDB	Q9KV29.
ProtClustDB	PRK00566.
Family and domain databases
HAMAP	MF_01322. RNApol_bact_RpoC. [Tree]
InterPro	IPR012754. DNA-dir_RpoC_beta_prime. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
KO	K03046.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02386. RpoC_TIGR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOC_VIBCH

Accession

Primary (citable) accession number: Q9KV29

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program