Q9KUY6 (ALR1_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase 1
EC=5.1.1.1

Gene names

Name:	alr1
Ordered Locus Names:	VC_0372

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.
Sequence caution	The sequence AAF93545.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Alanine racemase 1 HAMAP MF_01201		PRO_0000114591
Sites
	Active site	34	1	Proton acceptor; specific for D-alanine By similarity
	Active site	254	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	34	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KUY6 [UniParc]. Last modified March 5, 2002. Version 2. Checksum: EBD185735204A735	FASTA	361	39,349
	10 20 30 40 50 60 MKAATAYINL EALQHNLQRV KQQAPESKIM AVVKANGYGH GLRHIARHAL GADAFGVARI 70 80 90 100 110 120 EEALQLRASG VVKPILLLEG FYSPGDLPVL VTNNIQTVVH CEEQLQALEQ AQLETPVMVW 130 140 150 160 170 180 LKVDSGMHRL GVRPEQYQDF VARLHQCENV AKPLRYMSHF GCADELDKST TVEQTELFLS 190 200 210 220 230 240 LTQGCQGERS LAASAGLLAW PQSQLEWVRP GIIMYGVSPF VEKSAVQLGY QPVMTLKSHL 250 260 270 280 290 300 IAVREVKAGE SVGYGGTWTS QRDTKIGVIA IGYGDGYPRT APNGTPVVVN GRRVPIAGRV 310 320 330 340 350 360 SMDMLTVDLG PDACDRVGDE AMLWGNELPV EEVAAHIGTI GYELVTKLTS RVEMSYYGAG V

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF93545.1. Different initiation.
PIR	H82329.
RefSeq	NP_230026.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KUY6.
SMR	Q9KUY6. Positions 3-356.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2615051.
GenomeReviews	Gene locus VC_0372 in contig AE003852_GR.
KEGG	vch:VC0372.
PATRIC	20079815. VBIVibCho83274_0348.
TIGR	VC_0372.
Phylogenomic databases
HOGENOM	HBG712172.
OMA	EGTPVWV.
PhylomeDB	Q9KUY6.
ProtClustDB	PRK00053.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR1_VIBCH

Accession

Primary (citable) accession number: Q9KUY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	January 25, 2012
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program