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Q9KUX4 (CYSJ_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sulfite reductase [NADPH] flavoprotein alpha-component
Short name=SiR-FP
EC=1.8.1.2
Gene names
Name:cysJ
Ordered Locus Names:VC_0384
OrganismVibrio cholerae
Taxonomic identifier666 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length614 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component By similarity. HAMAP MF_01541

Catalytic activity

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01541

Cofactor

Binds 1 FAD per subunit By similarity. HAMAP MF_01541

Binds 1 FMN per subunit By similarity. HAMAP MF_01541

Pathway

Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01541

Subunit structure

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.

Sequence similarities

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 614614Sulfite reductase [NADPH] flavoprotein alpha-component HAMAP MF_01541
PRO_0000199939

Regions

Domain79 – 217139Flavodoxin-like
Domain249 – 463215FAD-binding FR-type
Nucleotide binding85 – 895FMN By similarity
Nucleotide binding132 – 1376FMN By similarity
Nucleotide binding165 – 19632FMN By similarity
Nucleotide binding401 – 4044FAD By similarity
Nucleotide binding435 – 4373FAD By similarity
Nucleotide binding534 – 5429NADP By similarity

Sites

Binding site5041NADP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KUX4 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 3081C655722FF44D

FASTA61467,702
        10         20         30         40         50         60 
MNREVVTMST GNTLPPALAA LASPLNDAQL NQLQQTVTQL NAQQLAWVSG YFWGLSQSNA 

        70         80         90        100        110        120 
LSVPHISAGQ TASAASGKLT IIFASQTGNA KGVAQALLKE AQAAGIQAQL FDASDYKGKD 

       130        140        150        160        170        180 
LAKETHVIFV ASTNGEGEAP DNALALHEFL KSKKAPKLPN LKYGVLGLGD SSYQFFCQTG 

       190        200        210        220        230        240 
KDFDQFLENL GAQRLVERLD ADVDYQAAAT EWRKQVLSIL KDELTGAAAV TSVATFAVSQ 

       250        260        270        280        290        300 
TAESHYSKEQ PYTASLSTSQ KITGRDSGKD VRHIEIDLAD SGITYQPGDA LGVWYENRPQ 

       310        320        330        340        350        360 
LVNALLDSVG LSGHEEVQVD GETLSLHSAL THHYEITAAN PQLVAQFAEL AQSEKLTSLA 

       370        380        390        400        410        420 
QDKEALREYA TRTQVIDVLR EEKVTLSAIQ LLSLLRRLTP RLYSIASSQS EVGEEVHLTV 

       430        440        450        460        470        480 
GVVEYEYEGE QRLGGASSFL AHQLEEGAPV KVFVEHNNNF KLPSDDNAPL IMVGPGTGIA 

       490        500        510        520        530        540 
PFRSFIQERE NRGAAGKNWL LFGDRTFTQD FLYQVEWQKY LKSGVLNRLD VAFSRDQHEK 

       550        560        570        580        590        600 
VYVQHRLLEQ AELVWQWLQE GAYFYVCGDA SRMAKDVHQA LITVVEQQGG LNREQAEEYV 

       610 
SELRKAKRYQ RDVY

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE003852 Genomic DNA. Translation: AAF93557.1.
PIRB82329.
RefSeqNP_230038.1. NC_002505.1.

3D structure databases

ProteinModelPortalQ9KUX4.
SMRQ9KUX4. Positions 245-614.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2615027.
GenomeReviewsGene locus VC_0384 in contig AE003852_GR.
KEGGvch:VC0384.
PATRIC20079839. VBIVibCho83274_0360.
TIGRVC_0384.

Phylogenomic databases

HOGENOMHBG736048.
OMAESADEYL.
PhylomeDBQ9KUX4.
ProtClustDBCLSK873955.

Family and domain databases

HAMAPMF_01541. CysJ.
[Tree]
InterProIPR010199. CysJ.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
Gene3DG3DSA:1.20.990.10. NADPH_Cyt_P450_Rdtase_dom3. 1 hit.
KOK00380.
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFPIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
TIGRFAMsTIGR01931. CysJ. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSJ_VIBCH
AccessionPrimary (citable) accession number: Q9KUX4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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