Reviewed, UniProtKB/Swiss-Prot Q9KUX2 (CYSH_VIBCH)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	VC_0386

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_0000100652

Sequences

	Sequence		Length	Mass (Da)
	Q9KUX2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A92EF4BEE08A9FFD	FASTA	253	29,249
	10 20 30 40 50 60 MPNRTVPTLE ELLTLNKVQQ TLRLTEVNQH LESLTAQERV VWGLENLQGN HALSSSFGIQ 70 80 90 100 110 120 AAVMLHLLTS VKSDIPVVLT DTGYLFPETY QFIDELTERL NLNLKVYSAP VSAAWQEARY 130 140 150 160 170 180 GKLWEQGVEG IERYNQINKV EPMRRALDEL NIGTWFSGLR REQSQSRASL PILSVQNGVF 190 200 210 220 230 240 KFLPVIDWTN KEVHYYLKDN DLPYHPLWEQ GYLSVGDTHT TQKWQPGMNE EQTRFFGLKR 250 ECGLHEDHND THQ

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003852 Genomic DNA. Translation: AAF93559.1.
PIR	D82329.
RefSeq	NP_230040.1.
3D structure databases
HSSP	HSSP built from PDB template 1SUR based on UniProtKB P17854.
ModBase	Search...
Genome annotation databases
GeneID	2615029.
GenomeReviews	Gene locus VC_0386 in contig AE003852_GR.
KEGG	vch:VC0386.
TIGR	VC_0386.
Phylogenomic databases
HOGENOM	Q9KUX2.
OMA	Q9KUX2. TRFNGLK.
Enzyme and pathway databases
BRENDA	1.8.4.8. 19019.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR004511. PAdo_PSO4_Rdtase_CysH. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_VIBCH

Accession

Primary (citable) accession number: Q9KUX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)