Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9KUT3

- MDH_VIBCH

UniProt

Q9KUT3 - MDH_VIBCH

Protein

Malate dehydrogenase

Gene

mdh

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341NADBy similarity
    Binding sitei81 – 811SubstrateBy similarity
    Binding sitei87 – 871SubstrateBy similarity
    Binding sitei94 – 941NADBy similarity
    Binding sitei119 – 1191SubstrateBy similarity
    Binding sitei153 – 1531SubstrateBy similarity
    Active sitei177 – 1771Proton acceptorBy similarity
    Binding sitei227 – 2271NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 137NADBy similarity
    Nucleotide bindingi117 – 1193NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP
    2. malic enzyme activity Source: TIGR

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: TIGR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase (EC:1.1.1.37)
    Gene namesi
    Name:mdh
    Ordered Locus Names:VC_0432
    OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
    Taxonomic identifieri243277 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000000584: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 311311Malate dehydrogenasePRO_0000113328Add
    BLAST

    Proteomic databases

    PRIDEiQ9KUT3.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9KUT3.
    SMRiQ9KUT3. Positions 1-310.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Phylogenomic databases

    KOiK00024.
    OMAiVEVKGFA.
    OrthoDBiEOG6091FG.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01516. Malate_dehydrog_1.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR023958. Malate_DH_type1_bac.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9KUT3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVAVIGAAG GIGQALALLL KNRLPAGSDL ALYDIAPVTP GVAADLSHIP    50
    TPVTIKGYAG EDPTPALEGA DVVLVSAGVA RKPGMDRADL FNVNAGIVKA 100
    LAEKIAVVCP KACVGIITNP VNTTVPIAAE VLKKAGVYDK RKLFGVTTLD 150
    VIRSETFVAA LKDKDPGQVR VPVIGGHSGV TILPLLSQVE GVSFTDEEVA 200
    ALTKRIQNAG TEVVEAKAGG GSATLSMGQA ACRFGLALVK ALQGESDVVE 250
    YAYVEGEGEY APFFAQPIKL GKNGVEALLD IGKLSAYEQA ALDGMLDTLK 300
    GDIQIGVEFV K 311
    Length:311
    Mass (Da):32,015
    Last modified:June 1, 2001 - v2
    Checksum:i5AD037C0E548BE9C
    GO

    Sequence cautioni

    The sequence AAF93605.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti63 – 631P → T in strain: M551.
    Natural varianti171 – 1711V → L in strain: M553, M535, CO391, CO407 and 653/36.
    Natural varianti204 – 2041K → N in strain: M548.
    Natural varianti223 – 2231A → T in strain: 569B.
    Natural varianti277 – 2771A → E in strain: M550, M551, M554, M557, M558 and GD98200.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF117859 Genomic DNA. Translation: AAD23488.1.
    AF117860 Genomic DNA. Translation: AAD23489.1.
    AF117861 Genomic DNA. Translation: AAD23490.1.
    AF117862 Genomic DNA. Translation: AAD23491.1.
    AF117863 Genomic DNA. Translation: AAD23492.1.
    AF117864 Genomic DNA. Translation: AAD23493.1.
    AF117865 Genomic DNA. Translation: AAD23494.1.
    AF117866 Genomic DNA. Translation: AAD23495.1.
    AF117867 Genomic DNA. Translation: AAD23496.1.
    AF117868 Genomic DNA. Translation: AAD23497.1.
    AF117869 Genomic DNA. Translation: AAD23498.1.
    AF117870 Genomic DNA. Translation: AAD23499.1.
    AF117871 Genomic DNA. Translation: AAD23500.1.
    AF117872 Genomic DNA. Translation: AAD23501.1.
    AF117873 Genomic DNA. Translation: AAD23502.1.
    AF117874 Genomic DNA. Translation: AAD23503.1.
    AF117875 Genomic DNA. Translation: AAD23504.1.
    AF117876 Genomic DNA. Translation: AAD23505.1.
    AF117877 Genomic DNA. Translation: AAD23506.1.
    AE003852 Genomic DNA. Translation: AAF93605.1. Different initiation.
    AF238329 Genomic DNA. Translation: AAK15054.1.
    AF238330 Genomic DNA. Translation: AAK15055.1.
    AF238331 Genomic DNA. Translation: AAK15056.1.
    AF238332 Genomic DNA. Translation: AAK15057.1.
    AF238333 Genomic DNA. Translation: AAK15058.1.
    AF238334 Genomic DNA. Translation: AAK15059.1.
    AF343280 Genomic DNA. Translation: AAK30532.1.
    AF343281 Genomic DNA. Translation: AAK30533.1.
    AF343282 Genomic DNA. Translation: AAK30534.1.
    AF343283 Genomic DNA. Translation: AAK30535.1.
    AF343284 Genomic DNA. Translation: AAK30536.1.
    AF343285 Genomic DNA. Translation: AAK30537.1.
    AF343286 Genomic DNA. Translation: AAK30538.1.
    AF343287 Genomic DNA. Translation: AAK30539.1.
    AF343288 Genomic DNA. Translation: AAK30540.1.
    AF343289 Genomic DNA. Translation: AAK30541.1.
    AF343290 Genomic DNA. Translation: AAK30542.1.
    AF343291 Genomic DNA. Translation: AAK30543.1.
    AF343292 Genomic DNA. Translation: AAK30544.1.
    AF343293 Genomic DNA. Translation: AAK30545.1.
    AF343294 Genomic DNA. Translation: AAK30546.1.
    AF343295 Genomic DNA. Translation: AAK30547.1.
    AF343296 Genomic DNA. Translation: AAK30548.1.
    AF343297 Genomic DNA. Translation: AAK30549.1.
    AF343298 Genomic DNA. Translation: AAK30550.1.
    AF343299 Genomic DNA. Translation: AAK30551.1.
    AF343300 Genomic DNA. Translation: AAK30552.1.
    AF343301 Genomic DNA. Translation: AAK30553.1.
    AF343302 Genomic DNA. Translation: AAK30554.1.
    AF343303 Genomic DNA. Translation: AAK30555.1.
    AF343304 Genomic DNA. Translation: AAK30556.1.
    AF343305 Genomic DNA. Translation: AAK30557.1.
    AF343306 Genomic DNA. Translation: AAK30558.1.
    AF343307 Genomic DNA. Translation: AAK30559.1.
    AF343308 Genomic DNA. Translation: AAK30560.1.
    AF343309 Genomic DNA. Translation: AAK30561.1.
    AF343310 Genomic DNA. Translation: AAK30562.1.
    AF540657 Genomic DNA. Translation: AAN23136.1.
    AF540658 Genomic DNA. Translation: AAN23137.1.
    AF540659 Genomic DNA. Translation: AAN23138.1.
    AF540660 Genomic DNA. Translation: AAN23139.1.
    PIRiG82324.
    RefSeqiNP_230086.2. NC_002505.1.

    Genome annotation databases

    EnsemblBacteriaiAAF93605; AAF93605; VC_0432.
    GeneIDi2615693.
    KEGGivch:VC0432.
    PATRICi20079943. VBIVibCho83274_0406.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF117859 Genomic DNA. Translation: AAD23488.1 .
    AF117860 Genomic DNA. Translation: AAD23489.1 .
    AF117861 Genomic DNA. Translation: AAD23490.1 .
    AF117862 Genomic DNA. Translation: AAD23491.1 .
    AF117863 Genomic DNA. Translation: AAD23492.1 .
    AF117864 Genomic DNA. Translation: AAD23493.1 .
    AF117865 Genomic DNA. Translation: AAD23494.1 .
    AF117866 Genomic DNA. Translation: AAD23495.1 .
    AF117867 Genomic DNA. Translation: AAD23496.1 .
    AF117868 Genomic DNA. Translation: AAD23497.1 .
    AF117869 Genomic DNA. Translation: AAD23498.1 .
    AF117870 Genomic DNA. Translation: AAD23499.1 .
    AF117871 Genomic DNA. Translation: AAD23500.1 .
    AF117872 Genomic DNA. Translation: AAD23501.1 .
    AF117873 Genomic DNA. Translation: AAD23502.1 .
    AF117874 Genomic DNA. Translation: AAD23503.1 .
    AF117875 Genomic DNA. Translation: AAD23504.1 .
    AF117876 Genomic DNA. Translation: AAD23505.1 .
    AF117877 Genomic DNA. Translation: AAD23506.1 .
    AE003852 Genomic DNA. Translation: AAF93605.1 . Different initiation.
    AF238329 Genomic DNA. Translation: AAK15054.1 .
    AF238330 Genomic DNA. Translation: AAK15055.1 .
    AF238331 Genomic DNA. Translation: AAK15056.1 .
    AF238332 Genomic DNA. Translation: AAK15057.1 .
    AF238333 Genomic DNA. Translation: AAK15058.1 .
    AF238334 Genomic DNA. Translation: AAK15059.1 .
    AF343280 Genomic DNA. Translation: AAK30532.1 .
    AF343281 Genomic DNA. Translation: AAK30533.1 .
    AF343282 Genomic DNA. Translation: AAK30534.1 .
    AF343283 Genomic DNA. Translation: AAK30535.1 .
    AF343284 Genomic DNA. Translation: AAK30536.1 .
    AF343285 Genomic DNA. Translation: AAK30537.1 .
    AF343286 Genomic DNA. Translation: AAK30538.1 .
    AF343287 Genomic DNA. Translation: AAK30539.1 .
    AF343288 Genomic DNA. Translation: AAK30540.1 .
    AF343289 Genomic DNA. Translation: AAK30541.1 .
    AF343290 Genomic DNA. Translation: AAK30542.1 .
    AF343291 Genomic DNA. Translation: AAK30543.1 .
    AF343292 Genomic DNA. Translation: AAK30544.1 .
    AF343293 Genomic DNA. Translation: AAK30545.1 .
    AF343294 Genomic DNA. Translation: AAK30546.1 .
    AF343295 Genomic DNA. Translation: AAK30547.1 .
    AF343296 Genomic DNA. Translation: AAK30548.1 .
    AF343297 Genomic DNA. Translation: AAK30549.1 .
    AF343298 Genomic DNA. Translation: AAK30550.1 .
    AF343299 Genomic DNA. Translation: AAK30551.1 .
    AF343300 Genomic DNA. Translation: AAK30552.1 .
    AF343301 Genomic DNA. Translation: AAK30553.1 .
    AF343302 Genomic DNA. Translation: AAK30554.1 .
    AF343303 Genomic DNA. Translation: AAK30555.1 .
    AF343304 Genomic DNA. Translation: AAK30556.1 .
    AF343305 Genomic DNA. Translation: AAK30557.1 .
    AF343306 Genomic DNA. Translation: AAK30558.1 .
    AF343307 Genomic DNA. Translation: AAK30559.1 .
    AF343308 Genomic DNA. Translation: AAK30560.1 .
    AF343309 Genomic DNA. Translation: AAK30561.1 .
    AF343310 Genomic DNA. Translation: AAK30562.1 .
    AF540657 Genomic DNA. Translation: AAN23136.1 .
    AF540658 Genomic DNA. Translation: AAN23137.1 .
    AF540659 Genomic DNA. Translation: AAN23138.1 .
    AF540660 Genomic DNA. Translation: AAN23139.1 .
    PIRi G82324.
    RefSeqi NP_230086.2. NC_002505.1.

    3D structure databases

    ProteinModelPortali Q9KUT3.
    SMRi Q9KUT3. Positions 1-310.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9KUT3.

    Protocols and materials databases

    DNASUi 2615693.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF93605 ; AAF93605 ; VC_0432 .
    GeneIDi 2615693.
    KEGGi vch:VC0432.
    PATRICi 20079943. VBIVibCho83274_0406.

    Phylogenomic databases

    KOi K00024.
    OMAi VEVKGFA.
    OrthoDBi EOG6091FG.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_01516. Malate_dehydrog_1.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR023958. Malate_DH_type1_bac.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolutionary relationships of pathogenic clones of Vibrio cholerae by sequence analysis of four housekeeping genes."
      Byun R., Elbourne L.D., Lan R., Reeves P.R.
      Infect. Immun. 67:1116-1124(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: M535, M536, M548, M549, M550, M551, M553, M554, M555, M556, M557, M558, M559, M560, M561, M562, M563, M645 and M793.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39315 / El Tor Inaba N16961.
    3. "Molecular analyses of a putative CTXphi precursor and evidence for independent acquisition of distinct CTXphis by toxigenic Vibrio cholerae."
      Boyd E.F., Heilpern A.J., Waldor M.K.
      J. Bacteriol. 182:5530-5538(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-248.
      Strain: 151 / Serotype O37, 208 / Serotype O11, ATCC 25870 / Classical Inaba 569B / Serotype O1, CO130 / Serotype O37, V46 / Serotype O141 and V52 / Serotype O37.
    4. "Allelic diversity and population structure in Vibrio cholerae O139 Bengal based on nucleotide sequence analysis."
      Farfan M., Minana-Galbis D., Fuste M.C., Loren J.G.
      J. Bacteriol. 184:1304-1313(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-201.
      Strain: 25872, 329 / Serotype O139, 653/36 / Serotype O139, BO1 / Serotype O139, BO2 / Serotype O139, BO4 / Serotype O139, CO391 / Serotype O139, CO396 / Serotype O139, CO402 / Serotype O139, CO403 / Serotype O139, CO404 / Serotype O139, CO406 / Serotype O139, CO407 / Serotype O139, CO414 / Serotype O139, CO415 / Serotype O139, CO418 / Serotype O139, CO487 / Serotype O1, MDO90 / Serotype O139, MOD084 / Serotype O139, NPO388 / Serotype O139, NPO390 / Serotype O139, NT329 / Serotype O139, NT330 / Serotype O139, NT638 / Serotype O139, NT642 / Serotype O139, NT648 / Serotype O139, SG24 / Serotype O139, SO19 / Serotype O139, SO29 / Serotype O139, SO30 / Serotype O139 and VO6 / Serotype O139.
    5. "Housekeeping genes of strains of Vibrio cholerae isolated from Guangdong province."
      Yongyu R., Biao K., Shouyi G.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 111-310.
      Strain: GD64313 / Serotype O1, GD9512 / Serotype O139, GD98200 / Serotype O139 and GD98224 / Serotype O1.

    Entry informationi

    Entry nameiMDH_VIBCH
    AccessioniPrimary (citable) accession number: Q9KUT3
    Secondary accession number(s): Q8GN36
    , Q8GN37, Q8GN38, Q8GN39, Q99PZ5, Q99Q77, Q99QN5, Q9AG49, Q9AG50, Q9AIU2, Q9R3N1, Q9R3N2, Q9R3N3, Q9S4R7, Q9XCZ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3