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Protein

Malate dehydrogenase

Gene

mdh

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADBy similarity
Binding sitei81 – 811SubstrateBy similarity
Binding sitei87 – 871SubstrateBy similarity
Binding sitei94 – 941NADBy similarity
Binding sitei119 – 1191SubstrateBy similarity
Binding sitei153 – 1531SubstrateBy similarity
Active sitei177 – 1771Proton acceptorBy similarity
Binding sitei227 – 2271NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 137NADBy similarity
Nucleotide bindingi117 – 1193NADBy similarity

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: UniProtKB-HAMAP
  • malic enzyme activity Source: TIGR

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • malate metabolic process Source: InterPro
  • tricarboxylic acid cycle Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
Ordered Locus Names:VC_0432
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Malate dehydrogenasePRO_0000113328Add
BLAST

Proteomic databases

PRIDEiQ9KUT3.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9KUT3.
SMRiQ9KUT3. Positions 1-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Phylogenomic databases

KOiK00024.
OMAiTECTYFS.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01516. Malate_dehydrog_1.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KUT3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVAVIGAAG GIGQALALLL KNRLPAGSDL ALYDIAPVTP GVAADLSHIP
60 70 80 90 100
TPVTIKGYAG EDPTPALEGA DVVLVSAGVA RKPGMDRADL FNVNAGIVKA
110 120 130 140 150
LAEKIAVVCP KACVGIITNP VNTTVPIAAE VLKKAGVYDK RKLFGVTTLD
160 170 180 190 200
VIRSETFVAA LKDKDPGQVR VPVIGGHSGV TILPLLSQVE GVSFTDEEVA
210 220 230 240 250
ALTKRIQNAG TEVVEAKAGG GSATLSMGQA ACRFGLALVK ALQGESDVVE
260 270 280 290 300
YAYVEGEGEY APFFAQPIKL GKNGVEALLD IGKLSAYEQA ALDGMLDTLK
310
GDIQIGVEFV K
Length:311
Mass (Da):32,015
Last modified:June 1, 2001 - v2
Checksum:i5AD037C0E548BE9C
GO

Sequence cautioni

The sequence AAF93605.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti63 – 631P → T in strain: M551.
Natural varianti171 – 1711V → L in strain: M553, M535, CO391, CO407 and 653/36.
Natural varianti204 – 2041K → N in strain: M548.
Natural varianti223 – 2231A → T in strain: 569B.
Natural varianti277 – 2771A → E in strain: M550, M551, M554, M557, M558 and GD98200.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117859 Genomic DNA. Translation: AAD23488.1.
AF117860 Genomic DNA. Translation: AAD23489.1.
AF117861 Genomic DNA. Translation: AAD23490.1.
AF117862 Genomic DNA. Translation: AAD23491.1.
AF117863 Genomic DNA. Translation: AAD23492.1.
AF117864 Genomic DNA. Translation: AAD23493.1.
AF117865 Genomic DNA. Translation: AAD23494.1.
AF117866 Genomic DNA. Translation: AAD23495.1.
AF117867 Genomic DNA. Translation: AAD23496.1.
AF117868 Genomic DNA. Translation: AAD23497.1.
AF117869 Genomic DNA. Translation: AAD23498.1.
AF117870 Genomic DNA. Translation: AAD23499.1.
AF117871 Genomic DNA. Translation: AAD23500.1.
AF117872 Genomic DNA. Translation: AAD23501.1.
AF117873 Genomic DNA. Translation: AAD23502.1.
AF117874 Genomic DNA. Translation: AAD23503.1.
AF117875 Genomic DNA. Translation: AAD23504.1.
AF117876 Genomic DNA. Translation: AAD23505.1.
AF117877 Genomic DNA. Translation: AAD23506.1.
AE003852 Genomic DNA. Translation: AAF93605.1. Different initiation.
AF238329 Genomic DNA. Translation: AAK15054.1.
AF238330 Genomic DNA. Translation: AAK15055.1.
AF238331 Genomic DNA. Translation: AAK15056.1.
AF238332 Genomic DNA. Translation: AAK15057.1.
AF238333 Genomic DNA. Translation: AAK15058.1.
AF238334 Genomic DNA. Translation: AAK15059.1.
AF343280 Genomic DNA. Translation: AAK30532.1.
AF343281 Genomic DNA. Translation: AAK30533.1.
AF343282 Genomic DNA. Translation: AAK30534.1.
AF343283 Genomic DNA. Translation: AAK30535.1.
AF343284 Genomic DNA. Translation: AAK30536.1.
AF343285 Genomic DNA. Translation: AAK30537.1.
AF343286 Genomic DNA. Translation: AAK30538.1.
AF343287 Genomic DNA. Translation: AAK30539.1.
AF343288 Genomic DNA. Translation: AAK30540.1.
AF343289 Genomic DNA. Translation: AAK30541.1.
AF343290 Genomic DNA. Translation: AAK30542.1.
AF343291 Genomic DNA. Translation: AAK30543.1.
AF343292 Genomic DNA. Translation: AAK30544.1.
AF343293 Genomic DNA. Translation: AAK30545.1.
AF343294 Genomic DNA. Translation: AAK30546.1.
AF343295 Genomic DNA. Translation: AAK30547.1.
AF343296 Genomic DNA. Translation: AAK30548.1.
AF343297 Genomic DNA. Translation: AAK30549.1.
AF343298 Genomic DNA. Translation: AAK30550.1.
AF343299 Genomic DNA. Translation: AAK30551.1.
AF343300 Genomic DNA. Translation: AAK30552.1.
AF343301 Genomic DNA. Translation: AAK30553.1.
AF343302 Genomic DNA. Translation: AAK30554.1.
AF343303 Genomic DNA. Translation: AAK30555.1.
AF343304 Genomic DNA. Translation: AAK30556.1.
AF343305 Genomic DNA. Translation: AAK30557.1.
AF343306 Genomic DNA. Translation: AAK30558.1.
AF343307 Genomic DNA. Translation: AAK30559.1.
AF343308 Genomic DNA. Translation: AAK30560.1.
AF343309 Genomic DNA. Translation: AAK30561.1.
AF343310 Genomic DNA. Translation: AAK30562.1.
AF540657 Genomic DNA. Translation: AAN23136.1.
AF540658 Genomic DNA. Translation: AAN23137.1.
AF540659 Genomic DNA. Translation: AAN23138.1.
AF540660 Genomic DNA. Translation: AAN23139.1.
PIRiG82324.
RefSeqiNP_230086.2. NC_002505.1.
WP_000861563.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93605; AAF93605; VC_0432.
GeneIDi2615693.
KEGGivch:VC0432.
PATRICi20079943. VBIVibCho83274_0406.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117859 Genomic DNA. Translation: AAD23488.1.
AF117860 Genomic DNA. Translation: AAD23489.1.
AF117861 Genomic DNA. Translation: AAD23490.1.
AF117862 Genomic DNA. Translation: AAD23491.1.
AF117863 Genomic DNA. Translation: AAD23492.1.
AF117864 Genomic DNA. Translation: AAD23493.1.
AF117865 Genomic DNA. Translation: AAD23494.1.
AF117866 Genomic DNA. Translation: AAD23495.1.
AF117867 Genomic DNA. Translation: AAD23496.1.
AF117868 Genomic DNA. Translation: AAD23497.1.
AF117869 Genomic DNA. Translation: AAD23498.1.
AF117870 Genomic DNA. Translation: AAD23499.1.
AF117871 Genomic DNA. Translation: AAD23500.1.
AF117872 Genomic DNA. Translation: AAD23501.1.
AF117873 Genomic DNA. Translation: AAD23502.1.
AF117874 Genomic DNA. Translation: AAD23503.1.
AF117875 Genomic DNA. Translation: AAD23504.1.
AF117876 Genomic DNA. Translation: AAD23505.1.
AF117877 Genomic DNA. Translation: AAD23506.1.
AE003852 Genomic DNA. Translation: AAF93605.1. Different initiation.
AF238329 Genomic DNA. Translation: AAK15054.1.
AF238330 Genomic DNA. Translation: AAK15055.1.
AF238331 Genomic DNA. Translation: AAK15056.1.
AF238332 Genomic DNA. Translation: AAK15057.1.
AF238333 Genomic DNA. Translation: AAK15058.1.
AF238334 Genomic DNA. Translation: AAK15059.1.
AF343280 Genomic DNA. Translation: AAK30532.1.
AF343281 Genomic DNA. Translation: AAK30533.1.
AF343282 Genomic DNA. Translation: AAK30534.1.
AF343283 Genomic DNA. Translation: AAK30535.1.
AF343284 Genomic DNA. Translation: AAK30536.1.
AF343285 Genomic DNA. Translation: AAK30537.1.
AF343286 Genomic DNA. Translation: AAK30538.1.
AF343287 Genomic DNA. Translation: AAK30539.1.
AF343288 Genomic DNA. Translation: AAK30540.1.
AF343289 Genomic DNA. Translation: AAK30541.1.
AF343290 Genomic DNA. Translation: AAK30542.1.
AF343291 Genomic DNA. Translation: AAK30543.1.
AF343292 Genomic DNA. Translation: AAK30544.1.
AF343293 Genomic DNA. Translation: AAK30545.1.
AF343294 Genomic DNA. Translation: AAK30546.1.
AF343295 Genomic DNA. Translation: AAK30547.1.
AF343296 Genomic DNA. Translation: AAK30548.1.
AF343297 Genomic DNA. Translation: AAK30549.1.
AF343298 Genomic DNA. Translation: AAK30550.1.
AF343299 Genomic DNA. Translation: AAK30551.1.
AF343300 Genomic DNA. Translation: AAK30552.1.
AF343301 Genomic DNA. Translation: AAK30553.1.
AF343302 Genomic DNA. Translation: AAK30554.1.
AF343303 Genomic DNA. Translation: AAK30555.1.
AF343304 Genomic DNA. Translation: AAK30556.1.
AF343305 Genomic DNA. Translation: AAK30557.1.
AF343306 Genomic DNA. Translation: AAK30558.1.
AF343307 Genomic DNA. Translation: AAK30559.1.
AF343308 Genomic DNA. Translation: AAK30560.1.
AF343309 Genomic DNA. Translation: AAK30561.1.
AF343310 Genomic DNA. Translation: AAK30562.1.
AF540657 Genomic DNA. Translation: AAN23136.1.
AF540658 Genomic DNA. Translation: AAN23137.1.
AF540659 Genomic DNA. Translation: AAN23138.1.
AF540660 Genomic DNA. Translation: AAN23139.1.
PIRiG82324.
RefSeqiNP_230086.2. NC_002505.1.
WP_000861563.1. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KUT3.
SMRiQ9KUT3. Positions 1-310.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9KUT3.

Protocols and materials databases

DNASUi2615693.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93605; AAF93605; VC_0432.
GeneIDi2615693.
KEGGivch:VC0432.
PATRICi20079943. VBIVibCho83274_0406.

Phylogenomic databases

KOiK00024.
OMAiTECTYFS.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01516. Malate_dehydrog_1.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evolutionary relationships of pathogenic clones of Vibrio cholerae by sequence analysis of four housekeeping genes."
    Byun R., Elbourne L.D., Lan R., Reeves P.R.
    Infect. Immun. 67:1116-1124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: M535, M536, M548, M549, M550, M551, M553, M554, M555, M556, M557, M558, M559, M560, M561, M562, M563, M645 and M793.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.
  3. "Molecular analyses of a putative CTXphi precursor and evidence for independent acquisition of distinct CTXphis by toxigenic Vibrio cholerae."
    Boyd E.F., Heilpern A.J., Waldor M.K.
    J. Bacteriol. 182:5530-5538(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-248.
    Strain: 151 / Serotype O37, 208 / Serotype O11, ATCC 25870 / Classical Inaba 569B / Serotype O1, CO130 / Serotype O37, V46 / Serotype O141 and V52 / Serotype O37.
  4. "Allelic diversity and population structure in Vibrio cholerae O139 Bengal based on nucleotide sequence analysis."
    Farfan M., Minana-Galbis D., Fuste M.C., Loren J.G.
    J. Bacteriol. 184:1304-1313(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-201.
    Strain: 25872, 329 / Serotype O139, 653/36 / Serotype O139, BO1 / Serotype O139, BO2 / Serotype O139, BO4 / Serotype O139, CO391 / Serotype O139, CO396 / Serotype O139, CO402 / Serotype O139, CO403 / Serotype O139, CO404 / Serotype O139, CO406 / Serotype O139, CO407 / Serotype O139, CO414 / Serotype O139, CO415 / Serotype O139, CO418 / Serotype O139, CO487 / Serotype O1, MDO90 / Serotype O139, MOD084 / Serotype O139, NPO388 / Serotype O139, NPO390 / Serotype O139, NT329 / Serotype O139, NT330 / Serotype O139, NT638 / Serotype O139, NT642 / Serotype O139, NT648 / Serotype O139, SG24 / Serotype O139, SO19 / Serotype O139, SO29 / Serotype O139, SO30 / Serotype O139 and VO6 / Serotype O139.
  5. "Housekeeping genes of strains of Vibrio cholerae isolated from Guangdong province."
    Yongyu R., Biao K., Shouyi G.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 111-310.
    Strain: GD64313 / Serotype O1, GD9512 / Serotype O139, GD98200 / Serotype O139 and GD98224 / Serotype O1.

Entry informationi

Entry nameiMDH_VIBCH
AccessioniPrimary (citable) accession number: Q9KUT3
Secondary accession number(s): Q8GN36
, Q8GN37, Q8GN38, Q8GN39, Q99PZ5, Q99Q77, Q99QN5, Q9AG49, Q9AG50, Q9AIU2, Q9R3N1, Q9R3N2, Q9R3N3, Q9S4R7, Q9XCZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: May 27, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.