Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9KUT3 (MDH_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:VC_0432
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01516

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01516

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01516

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Sequence caution

The sequence AAF93605.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311Malate dehydrogenase HAMAP-Rule MF_01516
PRO_0000113328

Regions

Nucleotide binding7 – 137NAD By similarity
Nucleotide binding117 – 1193NAD By similarity

Sites

Active site1771Proton acceptor By similarity
Binding site341NAD By similarity
Binding site811Substrate By similarity
Binding site871Substrate By similarity
Binding site941NAD By similarity
Binding site1191Substrate By similarity
Binding site1531Substrate By similarity
Binding site2271NAD By similarity

Natural variations

Natural variant631P → T in strain: M551.
Natural variant1711V → L in strain: M553, M535, CO391, CO407 and 653/36.
Natural variant2041K → N in strain: M548.
Natural variant2231A → T in strain: 569B.
Natural variant2771A → E in strain: M550, M551, M554, M557, M558 and GD98200.

Sequences

Sequence LengthMass (Da)Tools
Q9KUT3 [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: 5AD037C0E548BE9C

FASTA31132,015
        10         20         30         40         50         60 
MKVAVIGAAG GIGQALALLL KNRLPAGSDL ALYDIAPVTP GVAADLSHIP TPVTIKGYAG 

        70         80         90        100        110        120 
EDPTPALEGA DVVLVSAGVA RKPGMDRADL FNVNAGIVKA LAEKIAVVCP KACVGIITNP 

       130        140        150        160        170        180 
VNTTVPIAAE VLKKAGVYDK RKLFGVTTLD VIRSETFVAA LKDKDPGQVR VPVIGGHSGV 

       190        200        210        220        230        240 
TILPLLSQVE GVSFTDEEVA ALTKRIQNAG TEVVEAKAGG GSATLSMGQA ACRFGLALVK 

       250        260        270        280        290        300 
ALQGESDVVE YAYVEGEGEY APFFAQPIKL GKNGVEALLD IGKLSAYEQA ALDGMLDTLK 

       310 
GDIQIGVEFV K 

« Hide

References

« Hide 'large scale' references
[1]"Evolutionary relationships of pathogenic clones of Vibrio cholerae by sequence analysis of four housekeeping genes."
Byun R., Elbourne L.D., Lan R., Reeves P.R.
Infect. Immun. 67:1116-1124(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: M535, M536, M548, M549, M550, M551, M553, M554, M555, M556, M557, M558, M559, M560, M561, M562, M563, M645 and M793.
[2]"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae."
Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. expand/collapse author list , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.
Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 39315 / El Tor Inaba N16961.
[3]"Molecular analyses of a putative CTXphi precursor and evidence for independent acquisition of distinct CTXphis by toxigenic Vibrio cholerae."
Boyd E.F., Heilpern A.J., Waldor M.K.
J. Bacteriol. 182:5530-5538(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-248.
Strain: 151 / Serotype O37, 208 / Serotype O11, ATCC 25870 / Classical Inaba 569B / Serotype O1, CO130 / Serotype O37, V46 / Serotype O141 and V52 / Serotype O37.
[4]"Allelic diversity and population structure in Vibrio cholerae O139 Bengal based on nucleotide sequence analysis."
Farfan M., Minana-Galbis D., Fuste M.C., Loren J.G.
J. Bacteriol. 184:1304-1313(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-201.
Strain: 25872, 329 / Serotype O139, 653/36 / Serotype O139, BO1 / Serotype O139, BO2 / Serotype O139, BO4 / Serotype O139, CO391 / Serotype O139, CO396 / Serotype O139, CO402 / Serotype O139, CO403 / Serotype O139, CO404 / Serotype O139, CO406 / Serotype O139, CO407 / Serotype O139, CO414 / Serotype O139, CO415 / Serotype O139, CO418 / Serotype O139, CO487 / Serotype O1, MDO90 / Serotype O139, MOD084 / Serotype O139, NPO388 / Serotype O139, NPO390 / Serotype O139, NT329 / Serotype O139, NT330 / Serotype O139, NT638 / Serotype O139, NT642 / Serotype O139, NT648 / Serotype O139, SG24 / Serotype O139, SO19 / Serotype O139, SO29 / Serotype O139, SO30 / Serotype O139 and VO6 / Serotype O139.
[5]"Housekeeping genes of strains of Vibrio cholerae isolated from Guangdong province."
Yongyu R., Biao K., Shouyi G.
Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 111-310.
Strain: GD64313 / Serotype O1, GD9512 / Serotype O139, GD98200 / Serotype O139 and GD98224 / Serotype O1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF117859 Genomic DNA. Translation: AAD23488.1.
AF117860 Genomic DNA. Translation: AAD23489.1.
AF117861 Genomic DNA. Translation: AAD23490.1.
AF117862 Genomic DNA. Translation: AAD23491.1.
AF117863 Genomic DNA. Translation: AAD23492.1.
AF117864 Genomic DNA. Translation: AAD23493.1.
AF117865 Genomic DNA. Translation: AAD23494.1.
AF117866 Genomic DNA. Translation: AAD23495.1.
AF117867 Genomic DNA. Translation: AAD23496.1.
AF117868 Genomic DNA. Translation: AAD23497.1.
AF117869 Genomic DNA. Translation: AAD23498.1.
AF117870 Genomic DNA. Translation: AAD23499.1.
AF117871 Genomic DNA. Translation: AAD23500.1.
AF117872 Genomic DNA. Translation: AAD23501.1.
AF117873 Genomic DNA. Translation: AAD23502.1.
AF117874 Genomic DNA. Translation: AAD23503.1.
AF117875 Genomic DNA. Translation: AAD23504.1.
AF117876 Genomic DNA. Translation: AAD23505.1.
AF117877 Genomic DNA. Translation: AAD23506.1.
AE003852 Genomic DNA. Translation: AAF93605.1. Different initiation.
AF238329 Genomic DNA. Translation: AAK15054.1.
AF238330 Genomic DNA. Translation: AAK15055.1.
AF238331 Genomic DNA. Translation: AAK15056.1.
AF238332 Genomic DNA. Translation: AAK15057.1.
AF238333 Genomic DNA. Translation: AAK15058.1.
AF238334 Genomic DNA. Translation: AAK15059.1.
AF343280 Genomic DNA. Translation: AAK30532.1.
AF343281 Genomic DNA. Translation: AAK30533.1.
AF343282 Genomic DNA. Translation: AAK30534.1.
AF343283 Genomic DNA. Translation: AAK30535.1.
AF343284 Genomic DNA. Translation: AAK30536.1.
AF343285 Genomic DNA. Translation: AAK30537.1.
AF343286 Genomic DNA. Translation: AAK30538.1.
AF343287 Genomic DNA. Translation: AAK30539.1.
AF343288 Genomic DNA. Translation: AAK30540.1.
AF343289 Genomic DNA. Translation: AAK30541.1.
AF343290 Genomic DNA. Translation: AAK30542.1.
AF343291 Genomic DNA. Translation: AAK30543.1.
AF343292 Genomic DNA. Translation: AAK30544.1.
AF343293 Genomic DNA. Translation: AAK30545.1.
AF343294 Genomic DNA. Translation: AAK30546.1.
AF343295 Genomic DNA. Translation: AAK30547.1.
AF343296 Genomic DNA. Translation: AAK30548.1.
AF343297 Genomic DNA. Translation: AAK30549.1.
AF343298 Genomic DNA. Translation: AAK30550.1.
AF343299 Genomic DNA. Translation: AAK30551.1.
AF343300 Genomic DNA. Translation: AAK30552.1.
AF343301 Genomic DNA. Translation: AAK30553.1.
AF343302 Genomic DNA. Translation: AAK30554.1.
AF343303 Genomic DNA. Translation: AAK30555.1.
AF343304 Genomic DNA. Translation: AAK30556.1.
AF343305 Genomic DNA. Translation: AAK30557.1.
AF343306 Genomic DNA. Translation: AAK30558.1.
AF343307 Genomic DNA. Translation: AAK30559.1.
AF343308 Genomic DNA. Translation: AAK30560.1.
AF343309 Genomic DNA. Translation: AAK30561.1.
AF343310 Genomic DNA. Translation: AAK30562.1.
AF540657 Genomic DNA. Translation: AAN23136.1.
AF540658 Genomic DNA. Translation: AAN23137.1.
AF540659 Genomic DNA. Translation: AAN23138.1.
AF540660 Genomic DNA. Translation: AAN23139.1.
PIRG82324.
RefSeqNP_230086.2. NC_002505.1.

3D structure databases

ProteinModelPortalQ9KUT3.
SMRQ9KUT3. Positions 1-310.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9KUT3.

Protocols and materials databases

DNASU2615693.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF93605; AAF93605; VC_0432.
GeneID2615693.
KEGGvch:VC0432.
PATRIC20079943. VBIVibCho83274_0406.

Phylogenomic databases

KOK00024.
OMAVEVKGFA.
OrthoDBEOG6091FG.
ProtClustDBPRK05086.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_01516. Malate_dehydrog_1.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDH_VIBCH
AccessionPrimary (citable) accession number: Q9KUT3
Secondary accession number(s): Q8GN36 expand/collapse secondary AC list , Q8GN37, Q8GN38, Q8GN39, Q99PZ5, Q99Q77, Q99QN5, Q9AG49, Q9AG50, Q9AIU2, Q9R3N1, Q9R3N2, Q9R3N3, Q9S4R7, Q9XCZ8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: February 19, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families