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Q9KUS1 (PDXA_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxythreonine-4-phosphate dehydrogenase

EC=1.1.1.262
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenase
Gene names
Name:pdxA
Ordered Locus Names:VC_0444
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP) By similarity. HAMAP-Rule MF_00536

Catalytic activity

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH. HAMAP-Rule MF_00536

Cofactor

Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt By similarity.

Pathway

Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5. HAMAP-Rule MF_00536

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00536

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00536.

Miscellaneous

The active site is located at the dimer interface By similarity.

Sequence similarities

Belongs to the PdxA family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3303304-hydroxythreonine-4-phosphate dehydrogenase HAMAP-Rule MF_00536
PRO_0000188833

Sites

Metal binding1651Divalent metal cation; shared with dimeric partner By similarity
Metal binding2101Divalent metal cation; shared with dimeric partner By similarity
Metal binding2661Divalent metal cation; shared with dimeric partner By similarity
Binding site1351Substrate By similarity
Binding site1361Substrate By similarity
Binding site2741Substrate By similarity
Binding site2831Substrate By similarity
Binding site2921Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KUS1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: EDA785D5D5513995

FASTA33035,605
        10         20         30         40         50         60 
MSSKRIIVTA GEPAGIGPDL VLALSAQDWP HQLVVCADKA LLAQRATQLG IQVKLLDYQR 

        70         80         90        100        110        120 
DNPVQAQQAG TLLVEHIPLA EPVVAGQLNP ANGHYVLKTL ERAAKGCMNG EFDAIVTGPV 

       130        140        150        160        170        180 
HKGVINRAGV AFSGHTEFFA EQSKTPLVVM MLATEGLRTA LVTTHLPLAE VPQAITCERL 

       190        200        210        220        230        240 
EQIVHILHKD LVEKFAIAEP KIYVCGLNPH AGEDGVLGMD EIETITPTLQ RLREQYGMQL 

       250        260        270        280        290        300 
VGPLPADTIF SEKYLQQADA VLGMYHDQVL PVLKYKGFGR SVNITLGLPF IRTSVDHGTA 

       310        320        330 
LDLAGTGQAD AGSFWTALAY AIELVDKKAQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE003852 Genomic DNA. Translation: AAF93617.1.
PIRC82323.
RefSeqNP_230098.1. NC_002505.1.

3D structure databases

ProteinModelPortalQ9KUS1.
SMRQ9KUS1. Positions 1-325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243277.VC0444.

Protocols and materials databases

DNASU2615776.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF93617; AAF93617; VC_0444.
GeneID2615776.
KEGGvch:VC0444.
PATRIC20079967. VBIVibCho83274_0418.

Phylogenomic databases

eggNOGCOG1995.
KOK00097.
OMADTLFQDK.
OrthoDBEOG6GN6ZC.

Enzyme and pathway databases

BioCycVCHO:VC0444-MONOMER.
UniPathwayUPA00244; UER00312.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
HAMAPMF_00536. PdxA.
InterProIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00557. pdxA. 1 hit.
ProtoNetSearch...

Entry information

Entry namePDXA_VIBCH
AccessionPrimary (citable) accession number: Q9KUS1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways