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Q9KUS1

- PDXA_VIBCH

UniProt

Q9KUS1 - PDXA_VIBCH

Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

    Catalytic activityi

    4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

    Cofactori

    Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei135 – 1351SubstrateUniRule annotation
    Binding sitei136 – 1361SubstrateUniRule annotation
    Metal bindingi165 – 1651Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi210 – 2101Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi266 – 2661Divalent metal cation; shared with dimeric partnerUniRule annotation
    Binding sitei274 – 2741SubstrateUniRule annotation
    Binding sitei283 – 2831SubstrateUniRule annotation
    Binding sitei292 – 2921SubstrateUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
    2. catalytic activity Source: TIGR
    3. cobalt ion binding Source: UniProtKB-HAMAP
    4. magnesium ion binding Source: UniProtKB-HAMAP
    5. NAD binding Source: InterPro
    6. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
    2. pyridoxine biosynthetic process Source: TIGR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

    Enzyme and pathway databases

    BioCyciVCHO:VC0444-MONOMER.
    UniPathwayiUPA00244; UER00312.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
    Alternative name(s):
    4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
    Gene namesi
    Name:pdxAUniRule annotation
    Ordered Locus Names:VC_0444
    OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
    Taxonomic identifieri243277 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000000584: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3303304-hydroxythreonine-4-phosphate dehydrogenasePRO_0000188833Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243277.VC0444.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9KUS1.
    SMRiQ9KUS1. Positions 1-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PdxA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1995.
    KOiK00097.
    OMAiDTLFQDK.
    OrthoDBiEOG6GN6ZC.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    HAMAPiMF_00536. PdxA.
    InterProiIPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view]
    PfamiPF04166. PdxA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00557. pdxA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9KUS1-1 [UniParc]FASTAAdd to Basket

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    MSSKRIIVTA GEPAGIGPDL VLALSAQDWP HQLVVCADKA LLAQRATQLG    50
    IQVKLLDYQR DNPVQAQQAG TLLVEHIPLA EPVVAGQLNP ANGHYVLKTL 100
    ERAAKGCMNG EFDAIVTGPV HKGVINRAGV AFSGHTEFFA EQSKTPLVVM 150
    MLATEGLRTA LVTTHLPLAE VPQAITCERL EQIVHILHKD LVEKFAIAEP 200
    KIYVCGLNPH AGEDGVLGMD EIETITPTLQ RLREQYGMQL VGPLPADTIF 250
    SEKYLQQADA VLGMYHDQVL PVLKYKGFGR SVNITLGLPF IRTSVDHGTA 300
    LDLAGTGQAD AGSFWTALAY AIELVDKKAQ 330
    Length:330
    Mass (Da):35,605
    Last modified:October 1, 2000 - v1
    Checksum:iEDA785D5D5513995
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE003852 Genomic DNA. Translation: AAF93617.1.
    PIRiC82323.
    RefSeqiNP_230098.1. NC_002505.1.

    Genome annotation databases

    EnsemblBacteriaiAAF93617; AAF93617; VC_0444.
    GeneIDi2615776.
    KEGGivch:VC0444.
    PATRICi20079967. VBIVibCho83274_0418.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE003852 Genomic DNA. Translation: AAF93617.1 .
    PIRi C82323.
    RefSeqi NP_230098.1. NC_002505.1.

    3D structure databases

    ProteinModelPortali Q9KUS1.
    SMRi Q9KUS1. Positions 1-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243277.VC0444.

    Protocols and materials databases

    DNASUi 2615776.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF93617 ; AAF93617 ; VC_0444 .
    GeneIDi 2615776.
    KEGGi vch:VC0444.
    PATRICi 20079967. VBIVibCho83274_0418.

    Phylogenomic databases

    eggNOGi COG1995.
    KOi K00097.
    OMAi DTLFQDK.
    OrthoDBi EOG6GN6ZC.

    Enzyme and pathway databases

    UniPathwayi UPA00244 ; UER00312 .
    BioCyci VCHO:VC0444-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    HAMAPi MF_00536. PdxA.
    InterProi IPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view ]
    Pfami PF04166. PdxA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00557. pdxA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39315 / El Tor Inaba N16961.

    Entry informationi

    Entry nameiPDXA_VIBCH
    AccessioniPrimary (citable) accession number: Q9KUS1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is located at the dimer interface.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3