Q9KUR1 (GLSA_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaminase
EC=3.5.1.2

Gene names

Name:	glsA
Ordered Locus Names:	VC_0454

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_00313
Sequence similarities	Belongs to the glutaminase family.
Sequence caution	The sequence AAF93627.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	catalytic activity Inferred from sequence or structural similarity Ref.1. Source: TIGR glutaminase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Glutaminase HAMAP-Rule MF_00313		PRO_0000110629
Sites
	Binding site	64	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	159	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity
	Binding site	242	1	Substrate By similarity
	Binding site	260	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KUR1 [UniParc]. Last modified June 16, 2003. Version 2. Checksum: 24FAE9E570A002B3	FASTA	306	32,765
	10 20 30 40 50 60 MKPTADILAS IIEEVRPLTS KGIVADYIPA LAKVPSDKLG IAVFTNQGEV ITAGDAQEGF 70 80 90 100 110 120 SIQSISKVLS LTLAMGLYQP DELWSRVGKE PSGQAFNSLI QLEMEQGIPR NPFINAGAIV 130 140 150 160 170 180 VCDMLQSRLS APRQRLLEFV RQLSGEPQIA YDKVVAASEM MHSDRNAAIA YLMRSFGNFH 190 200 210 220 230 240 NEVIPVLHNY FHACALKMSC VELAKTFSYL ANKGVSVVTG ETVITPTQSK QTNALLATCG 250 260 270 280 290 300 LYDGAGEFAY RVGMPGKSGV GGGIIAVVPG EMTIAVWSPA LDQSGNSLAG TRALELLAQR IGRSIF

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF93627.1. Different initiation.
PIR	F82320.
RefSeq	NP_230108.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KUR1.
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VC0454.
Protocols and materials databases
DNASU	2615116.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF93627; AAF93627; VC_0454.
GeneID	2615116.
KEGG	vch:VC0454.
PATRIC	20080003. VBIVibCho83274_0427.
Phylogenomic databases
eggNOG	COG2066.
KO	K01425.
OMA	EPSGNPF.
ProtClustDB	PRK00971.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
HAMAP	MF_00313. Glutaminase.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR015868. Glutaminase. [Graphical view]
PANTHER	PTHR12544. PTHR12544. 1 hit.
Pfam	PF04960. Glutaminase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
TIGRFAMs	TIGR03814. Gln_ase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLSA_VIBCH

Accession

Primary (citable) accession number: Q9KUR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	June 16, 2003
Last modified:	May 1, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program