Q9KUQ9 (NTPA_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

VC_0456

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178260
Regions
	Region	8 – 13	6	Substrate binding By similarity
	Region	69 – 70	2	Substrate binding By similarity
Sites
	Metal binding	69	1	Magnesium or manganese By similarity
	Binding site	157	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KUQ9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 139C41F236AEC3D4	FASTA	200	21,936
	10 20 30 40 50 60 MKKIVLATGN QGKVREMADL LSDFGFDVVA QSEFNVPEAA ETGTTFIENA IIKARHAAQI 70 80 90 100 110 120 TGLPTIADDS GLEVDYLNGA PGIYSARYAG EHASDGDNLN KLLMAMQDVP DDQRSARFHC 130 140 150 160 170 180 VLVLMRHADD PTPIVCHGKW EGKILTAPHG SNGFGYDPIF WVPEENCASA ELEPVRKKQL 190 200 SHRGKALQKL FKAIEEQRTC

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF93629.1.
PIR	H82320.
RefSeq	NP_230110.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KUQ9.
SMR	Q9KUQ9. Positions 1-194.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2615118.
GenomeReviews	Gene locus VC_0456 in contig AE003852_GR.
KEGG	vch:VC0456.
PATRIC	20080007. VBIVibCho83274_0429.
TIGR	VC_0456.
Phylogenomic databases
HOGENOM	HBG697237.
OMA	YSKRYDQ.
PhylomeDB	Q9KUQ9.
ProtClustDB	PRK00120.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_VIBCH

Accession

Primary (citable) accession number: Q9KUQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program