Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosine--tRNA ligase 1

Gene

tyrS1

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36TyrosineUniRule annotation1
Binding sitei175TyrosineUniRule annotation1
Binding sitei179TyrosineUniRule annotation1
Binding sitei238ATPUniRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • RNA binding Source: UniProtKB-KW
  • tyrosine-tRNA ligase activity Source: TIGR

GO - Biological processi

  • tRNA aminoacylation Source: GO_Central
  • tyrosyl-tRNA aminoacylation Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligase 1UniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetase 1UniRule annotation
Short name:
TyrRS 1UniRule annotation
Gene namesi
Name:tyrS1UniRule annotation
Ordered Locus Names:VC_0465
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002348081 – 426Tyrosine--tRNA ligase 1Add BLAST426

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243277.VC0465.

Structurei

3D structure databases

ProteinModelPortaliQ9KUQ0.
SMRiQ9KUQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini357 – 421S4 RNA-bindingUniRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 50"HIGH" region10
Motifi235 – 239"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily.UniRule annotation
Contains 1 S4 RNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DA0. Bacteria.
COG0162. LUCA.
KOiK01866.
OMAiGKHFPVN.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KUQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSQQLFDDL QQRGLIAQST PLEALVERFA TPQVLYCGFD PTAGSLHIGH
60 70 80 90 100
LVPLLMLKRF QNAGHQAIAL IGGATGMIGD PSFKANERVL NSAQTVQQWV
110 120 130 140 150
SDLSQQITQL MTSQGENCAS LMAVNNADWI GKINLIDFFR DVGKHFSVNA
160 170 180 190 200
MINRESVKQR LARPDQGISF TEFSYSLLQS YDFAHLNREF GCALQIGGND
210 220 230 240 250
QWGNIVSGID LTRRLNGVEV CGLTLPLITK SDGTKFGKTE SGAVWLESSK
260 270 280 290 300
TSPYRFYQFW LNSDDADVYR FLAYYTFLST AEIEEIRQAD ALRAGKPQAQ
310 320 330 340 350
QILAEHVTRF VHGEEGLAAA QRITQALFSG DVSYLSLSEL AQLELDGLPC
360 370 380 390 400
VAASRTDSLL DLLLSSGLAS SKRQAREWLE NGAIRVNGER VQQEGGLSGF
410 420
ALFDRYYVLQ RGKKQFALIK LEGLAE
Length:426
Mass (Da):47,119
Last modified:May 16, 2006 - v2
Checksum:i7338212186058F4B
GO

Sequence cautioni

The sequence AAF93638 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93638.1. Different initiation.
PIRiF82318.
RefSeqiNP_230119.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93638; AAF93638; VC_0465.
GeneIDi2615127.
KEGGivch:VC0465.
PATRICi20080025. VBIVibCho83274_0438.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93638.1. Different initiation.
PIRiF82318.
RefSeqiNP_230119.1. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KUQ0.
SMRiQ9KUQ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0465.

Protocols and materials databases

DNASUi2615127.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93638; AAF93638; VC_0465.
GeneIDi2615127.
KEGGivch:VC0465.
PATRICi20080025. VBIVibCho83274_0438.

Phylogenomic databases

eggNOGiENOG4105DA0. Bacteria.
COG0162. LUCA.
KOiK01866.
OMAiGKHFPVN.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYY1_VIBCH
AccessioniPrimary (citable) accession number: Q9KUQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 16, 2006
Last modified: November 30, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.