Reviewed, UniProtKB/Swiss-Prot Q9KUQ0 (SYY1_VIBCH)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase 1
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase 1
      Short name=TyrRS 1

Gene names

Name:	tyrS1
Ordered Locus Names:	VC_0465

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Tyrosyl-tRNA synthetase 1 HAMAP MF_02006		PRO_0000234808
Regions
	Domain	357 – 421	65	S4 RNA-binding
	Motif	41 – 50	10	"HIGH" region HAMAP MF_02006
	Motif	235 – 239	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	36	1	Tyrosine By similarity
	Binding site	175	1	Tyrosine By similarity
	Binding site	179	1	Tyrosine By similarity
	Binding site	238	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KUQ0-1 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 7338212186058F4B	FASTA	426	47,119
	10 20 30 40 50 60 MNSQQLFDDL QQRGLIAQST PLEALVERFA TPQVLYCGFD PTAGSLHIGH LVPLLMLKRF 70 80 90 100 110 120 QNAGHQAIAL IGGATGMIGD PSFKANERVL NSAQTVQQWV SDLSQQITQL MTSQGENCAS 130 140 150 160 170 180 LMAVNNADWI GKINLIDFFR DVGKHFSVNA MINRESVKQR LARPDQGISF TEFSYSLLQS 190 200 210 220 230 240 YDFAHLNREF GCALQIGGND QWGNIVSGID LTRRLNGVEV CGLTLPLITK SDGTKFGKTE 250 260 270 280 290 300 SGAVWLESSK TSPYRFYQFW LNSDDADVYR FLAYYTFLST AEIEEIRQAD ALRAGKPQAQ 310 320 330 340 350 360 QILAEHVTRF VHGEEGLAAA QRITQALFSG DVSYLSLSEL AQLELDGLPC VAASRTDSLL 370 380 390 400 410 420 DLLLSSGLAS SKRQAREWLE NGAIRVNGER VQQEGGLSGF ALFDRYYVLQ RGKKQFALIK LEGLAE

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003852 Genomic DNA. Translation: AAF93638.1. Different initiation.
PIR	F82318.
RefSeq	NP_230119.1.
3D structure databases
HSSP	HSSP built from PDB template 2TS1 based on UniProtKB P00952.
ModBase	Search...
Genome annotation databases
GeneID	2615127.
GenomeReviews	Gene locus VC_0465 in contig AE003852_GR.
KEGG	vch:VC0465.
TIGR	VC_0465.
Phylogenomic databases
HOGENOM	Q9KUQ0.
Enzyme and pathway databases
BRENDA	6.1.1.1. 19019.
Family and domain databases
HAMAP	MF_02006. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY1_VIBCH

Accession

Primary (citable) accession number: Q9KUQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)