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Q9KUP7 (GSHB_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:VC_0468
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197491

Regions

Domain124 – 310187ATP-grasp
Nucleotide binding150 – 20758ATP By similarity

Sites

Metal binding2811Magnesium or manganese By similarity
Metal binding2831Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KUP7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 47C50BAC0E2693AF

FASTA31835,408
        10         20         30         40         50         60 
MIKLGIVMDP IASINIKKDS SFAMMLEAQR RGYEIHYMEM NDLHLEQGVA LADTKVVELK 

        70         80         90        100        110        120 
EDPSGWYQFK SEQTIALSEL DAILMRKDPP FDTEYIYATY ILERAEDEGV LVVNKPQSLR 

       130        140        150        160        170        180 
DCNEKLFTAW FPELTPITMV TRKAEKIKAF REQHGDVILK PLDGMGGASI FRVKEGDPNL 

       190        200        210        220        230        240 
SVIIETLTNH GQNYCMAQTF VPDISNGDKR ILVVDGEPMP YCLARIPAKG ETRGNLAAGG 

       250        260        270        280        290        300 
RGEPRPLSET DKKIALAVAP TLKEKGLLFV GLDVIGDKLT EINVTSPTCI REIEAAYDIS 

       310 
ITGKLMDAIE RRLKATAM 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE003852 Genomic DNA. Translation: AAF93641.1.
PIRA82319.
RefSeqNP_230122.1. NC_002505.1.

3D structure databases

ProteinModelPortalQ9KUP7.
SMRQ9KUP7. Positions 1-314.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243277.VC0468.

Proteomic databases

PRIDEQ9KUP7.

Protocols and materials databases

DNASU2615130.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF93641; AAF93641; VC_0468.
GeneID2615130.
KEGGvch:VC0468.
PATRIC20080031. VBIVibCho83274_0441.

Phylogenomic databases

eggNOGCOG0189.
KOK01920.
OMADWKIARA.
OrthoDBEOG6WMHWB.

Enzyme and pathway databases

BioCycVCHO:VC0468-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_VIBCH
AccessionPrimary (citable) accession number: Q9KUP7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways