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Q9KUG1 (Q9KUG1_VIBCH) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Signal recognition particle protein HAMAP-Rule MF_00306
Alternative name(s):
Fifty-four homolog HAMAP-Rule MF_00306
Gene names
Name:ffh HAMAP-Rule MF_00306
Ordered Locus Names:VC_0560
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio ›

Protein attributes

Sequence length461 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components By similarity. HAMAP-Rule MF_00306

Subunit structure

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule By similarity. HAMAP-Rule MF_00306

Subcellular location

Cytoplasm. Note: The SRP-RNC complex is targeted to the cytoplasmic membrane By similarity. HAMAP-Rule MF_00306

Domain

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC By similarity. HAMAP-Rule MF_00306

Sequence similarities

Belongs to the GTP-binding SRP family. SRP54 subfamily. HAMAP-Rule MF_00306

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding107 – 1148GTP By similarity HAMAP-Rule MF_00306
Nucleotide binding190 – 1945GTP By similarity HAMAP-Rule MF_00306
Nucleotide binding248 – 2514GTP By similarity HAMAP-Rule MF_00306

Sequences

Sequence LengthMass (Da)Tools
Q9KUG1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 616E9291972B3CDB

FASTA46150,374
        10         20         30         40         50         60 
MFENLTDRLS KTLKNISGKG RLTEDNIKDT LREVRMALLE ADVALPVVRD FVNRVKEGAV 

        70         80         90        100        110        120 
GVEVSKSLTP GQEFIKIVRQ QLEAVMGESN EALNLAAQPP AVILMAGLQG AGKTTSVGKL 

       130        140        150        160        170        180 
SKLLKERDKK KVLVVSADVY RPAAIKQLET LANDLGVDFF PSTPDQKPID IANAAIDHAK 

       190        200        210        220        230        240 
KKFYDVLIVD TAGRLAIDEQ MMAEIQALHK AINPVETLFV VDAMTGQDAA DTAKAFGDAL 

       250        260        270        280        290        300 
PLTGVILTKV DGDARGGAAL SVRHITGKPI KFIGVGEKTD ALEPFHPDRI ASRILGMGDV 

       310        320        330        340        350        360 
LSLIEDLQRN VDQEKAEKLA KKFKEKKGFD LEDFREQLGQ MKNMGGMMGM LDKLPGMSQL 

       370        380        390        400        410        420 
PADMKDKVDD RVFKQMEAII NSMTMKERQN PDLIKGSRKK RIAAGSGTQV QDVNRLLKQF 

       430        440        450        460 
TQMQKMMKKM QQGGMKGMMR NMQGLMGGGG RGGFGGGFFG R

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE003852 Genomic DNA. Translation: AAF93728.1.
PIRH82306.
RefSeqNP_230211.1. NC_002505.1.

3D structure databases

HSSPHSSP built from PDB template 1HQ1 based on UniProtKB P07019.
ProteinModelPortalQ9KUG1.
SMRQ9KUG1. Positions 2-431.
ModBaseSearch...

Protein-protein interaction databases

STRING243277.VC0560.

Protocols and materials databases

DNASU2615237.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF93728; AAF93728; VC_0560.
GeneID2615237.
KEGGvch:VC0560.
PATRIC20080238. VBIVibCho83274_0535.

Phylogenomic databases

KOK03106.
OMAGQQVVKI.
ProtClustDBPRK10867.

Family and domain databases

Gene3D1.10.260.30. 1 hit.
HAMAPMF_00306. SRP54.
InterProIPR003593. AAA+_ATPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
IPR004780. SRP_Ffh.
[Graphical view]
PANTHERPTHR11564:SF7. PTHR11564:SF7. 1 hit.
PfamPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMSSF47446. Signal_recog_particle_SRP54_M. 1 hit.
SSF47364. SRP54. 1 hit.
TIGRFAMsTIGR00959. ffh. 1 hit.
PROSITEPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ9KUG1_VIBCH
AccessionPrimary (citable) accession number: Q9KUG1
Entry history
Integrated into UniProtKB/TrEMBL: October 1, 2000
Last sequence update: October 1, 2000
Last modified: May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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