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Protein

Penicillin-binding protein 1B

Gene

mrcB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (By similarity).By similarity

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei200Proton donor; for transglycosylase activityBy similarity1
Active sitei476Acyl-ester intermediate; for transpeptidase activityBy similarity1

GO - Molecular functioni

  • catalytic activity Source: TIGR
  • penicillin binding Source: InterPro
  • peptidase activity Source: InterPro
  • peptidoglycan glycosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciVCHO:VC0602-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1B
Short name:
PBP-1b
Short name:
PBP1b
Alternative name(s):
Murein polymerase
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.1.129)
Alternative name(s):
Peptidoglycan TGase
Peptidoglycan glycosyltransferase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:mrcB
Synonyms:ponB
Ordered Locus Names:VC_0602
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30CytoplasmicSequence analysisAdd BLAST30
Transmembranei31 – 52Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST22
Topological domaini53 – 777PeriplasmicSequence analysisAdd BLAST725

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000831871 – 777Penicillin-binding protein 1BAdd BLAST777

Interactioni

Protein-protein interaction databases

STRINGi243277.VC0602.

Structurei

3D structure databases

ProteinModelPortaliQ9KUC0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni162 – 334TransglycosylaseAdd BLAST173
Regioni415 – 709TranspeptidaseAdd BLAST295

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ4. Bacteria.
COG0744. LUCA.
KOiK05365.
OMAiPHLESAM.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011813. PBP_1b.
IPR001460. PCN-bd_Tpept.
IPR028166. UB2H.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
PF14814. UB2H. 1 hit.
[Graphical view]
PIRSFiPIRSF002799. PBP_1b. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
TIGRFAMsiTIGR02071. PBP_1b. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KUC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRKSSNRSR GRKARSGKSA SSSKLQIWLG RIWSIGWKLA LTLAAVLVFI
60 70 80 90 100
GIYLDSMIKQ RFEGQLFDLP TVVYARILTL EPGNGLSLQE LRNELDVLNY
110 120 130 140 150
RKVAQPRFPG EYASSSSRIE LIRRPFEFAD GPEPDRRVML TFDGSGLNKI
160 170 180 190 200
ESLEQKRELG YLRLEPKLMG MLEKDSPEQR LFLRRDQFPE VLVDALLVTE
210 220 230 240 250
DRDFYQHDGV SPLAIGRAMV VNLKAGRTVQ GGSTLTQQLA KNIFLSSDRT
260 270 280 290 300
LWRKLREAYM ALIIDYRYSK DRILEAYLNE VYLGQSGADA IHGFGLASRL
310 320 330 340 350
YFGQPLQELR IDQLALLVGM VKGPSYYNPM RYAERARERR DLVLKLMMEH
360 370 380 390 400
DILTAPEYQQ AVTRPLDVQK TAQIASRQPA YFQQVSIELK EKLGDKFKAD
410 420 430 440 450
SGLRVFTSLD PVSQSKLEQA IHDQIPQLAK TAGKDLEAAA IAVDRHSGEI
460 470 480 490 500
RAMVGGKRTG YDGFNRVLNA SRQIGSLVKP AVYLTALAHP DQYNLATTLE
510 520 530 540 550
DKPLTLKGSE GSAWTPRNYD RQYRGEVPLY LALAQSLNVP TVALGMKLGI
560 570 580 590 600
DQVSATLGKL GVNRDEIRPV PSMLLGSFSL TPYQVAQMYQ TLTNSGKKAP
610 620 630 640 650
LSALRSVLDL EGNVLYESLP RVSQAVDQQA AWLTTYAMKQ GVQEGTGRYL
660 670 680 690 700
NAQFSSAALA GKTGTTNDNR DSWFVGVDGR EVTTIWLGRD DNQPTKLTGA
710 720 730 740 750
SGALRVYAQY LKYRIPEKLQ LPWPEGITTF GFAKQTQGGL KLDCDNAFKL
760 770
PIWDNQQQLK QQCENRPTEW IKKLFEW
Length:777
Mass (Da):87,324
Last modified:October 1, 2000 - v1
Checksum:iBCFC2676EA2AFF1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93769.1.
PIRiA82303.
RefSeqiNP_230252.1. NC_002505.1.
WP_000197283.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93769; AAF93769; VC_0602.
GeneIDi2615390.
KEGGivch:VC0602.
PATRICi20080312. VBIVibCho83274_0572.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93769.1.
PIRiA82303.
RefSeqiNP_230252.1. NC_002505.1.
WP_000197283.1. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KUC0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0602.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Protocols and materials databases

DNASUi2615390.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93769; AAF93769; VC_0602.
GeneIDi2615390.
KEGGivch:VC0602.
PATRICi20080312. VBIVibCho83274_0572.

Phylogenomic databases

eggNOGiENOG4105BZ4. Bacteria.
COG0744. LUCA.
KOiK05365.
OMAiPHLESAM.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciVCHO:VC0602-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011813. PBP_1b.
IPR001460. PCN-bd_Tpept.
IPR028166. UB2H.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
PF14814. UB2H. 1 hit.
[Graphical view]
PIRSFiPIRSF002799. PBP_1b. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
TIGRFAMsiTIGR02071. PBP_1b. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPBPB_VIBCH
AccessioniPrimary (citable) accession number: Q9KUC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.