Q9KU92 (SYY2_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase 2
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase 2
Short name=TyrRS 2

Gene names

Name:	tyrS2
Ordered Locus Names:	VC_0631

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02007
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity. HAMAP MF_02007
Subcellular location	Cytoplasm By similarity HAMAP MF_02007.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Tyrosine--tRNA ligase 2 HAMAP MF_02007		PRO_0000236776
Regions
	Domain	334 – 394	61	S4 RNA-binding
	Motif	42 – 51	10	"HIGH" region HAMAP MF_02007
	Motif	226 – 230	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	229	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KU92 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E53F44FD84FADD43	FASTA	395	44,075
	10 20 30 40 50 60 MASIEAALAE IKRGVEELIP EEELIAKLKE NRPLRIKLGA DPTAPDIHLG HTVILNKLRT 70 80 90 100 110 120 FQDLGHDVTF LIGDFTGMVG DPTGKNTTRP PLTREDVLRN AETYKQQVFK ILDPAKTKIQ 130 140 150 160 170 180 FNSEWLSKLG AEGMIRLASN QTVARMLERD DFKKRYNNGQ PIAIHEFMYP LLQGYDSVAM 190 200 210 220 230 240 ETDVELGGTD QKFNLLMGRE LQKANGQKPQ VVLMMPLLVG LDGEKKMSKS AHNYIGVSEA 250 260 270 280 290 300 PSEMFGKIMS ISDDLMWSYY ELLSFRPLEE VAQFKAEVAN GANPRDIKIL LAKEIIARFH 310 320 330 340 350 360 SQADADAAEQ EFINRFQKGA MPEEMPEFEF ESGIAISNLL KEAGLVASTS DALRMIKQGA 370 380 390 VKLDGEKLED AKLIPACGTS VYQVGKRKFA RVTIK

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF93797.1.
PIR	G82298.
RefSeq	NP_230280.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KU92.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2615419.
GenomeReviews	Gene locus VC_0631 in contig AE003852_GR.
KEGG	vch:VC0631.
PATRIC	20080371. VBIVibCho83274_0601.
TIGR	VC_0631.
Phylogenomic databases
HOGENOM	HBG288125.
OMA	YVVQVGK.
PhylomeDB	Q9KU92.
ProtClustDB	PRK05912.
Family and domain databases
HAMAP	MF_02007. Tyr_tRNA_synth_type2. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024108. Tyr-tRNA-synth_bac_2. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY2_VIBCH

Accession

Primary (citable) accession number: Q9KU92

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program