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Q9KU47 (SYI_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:VC_0682
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length949 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 949949Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098497

Regions

Motif58 – 6811"HIGH" region HAMAP-Rule MF_02002
Motif608 – 6125"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding9121Zinc By similarity
Metal binding9151Zinc By similarity
Metal binding9321Zinc By similarity
Metal binding9351Zinc By similarity
Binding site5671Aminoacyl-adenylate By similarity
Binding site6111ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KU47 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: F520EDBE6A91DDE5

FASTA949105,399
        10         20         30         40         50         60 
MSEYKDTLNL PETGFPMRGD LAKREPEMLQ RWYQEDLYGA IRQAKKGKKS FVLHDGPPYA 

        70         80         90        100        110        120 
NGDIHIGHAL NKILKDVIIK SKTLSGFDAP YIPGWDCHGL PIELMVEKKV GKPGQKVTAA 

       130        140        150        160        170        180 
EFREKCREYA AGQVEGQKES FKRLGILGEW DKPYRTMDFV TEANIIRALG KIADNGHLLK 

       190        200        210        220        230        240 
GFKPVHWCTD CGSALAEAEV EYKNKVSPSI DVRFKAADEA AVLAKFGLAA GHEGKGDVSI 

       250        260        270        280        290        300 
VIWTTTPWTL PANRAVCLRA DLEYVLIQVE GEQPERIIVA SELAKSVMDR AGIEHFHNLG 

       310        320        330        340        350        360 
FATGADLELV QFQHPFYSFT VPAILGDHVT TDSGTGVVHT APGHGQEDFA VGQQYGLEVA 

       370        380        390        400        410        420 
NPVGSNGVYL PDTELFAGQH VFKANDSVLE VLKEKGALLH HHAYEHSYPH CWRHKTPIIF 

       430        440        450        460        470        480 
RATPQWFVSM EQAGLREQAL TAIKGVHWMP DWGQSRIEGM VAGRPEWCIS RQRTWGVPIA 

       490        500        510        520        530        540 
LFVHKETAEL HPNSADLIEK VAQLVEQKGI QAWWDLDTAE LLGAEDAANY EKVLDTLDVW 

       550        560        570        580        590        600 
FDSGVTHSAV VDARQEFNGA EADMYLEGSD QHRGWFQSSL ISSVAMKGKA PYKEVLTHGF 

       610        620        630        640        650        660 
VVDGQGRKMS KSIGNVVAPQ DVTNKLGADI LRLWVASTDY TGEVAVSDEI LKRSADAYRR 

       670        680        690        700        710        720 
IRNTARFFLA NLNGFNPTTD IIPVEDMVAL DRWAVGRALA AQQEIIQAYQ DYNLHAVVQR 

       730        740        750        760        770        780 
LMNFCSIEMG SFYLDVIKDR QYTAKRGGHA QRSCQTALFF IVEALVRWMA PIMSFTADEI 

       790        800        810        820        830        840 
WNAMPAQQAD GSARDKFVFT TEWFDGLFGL AEGEELNNAF WNDIQKVRGS VNKLLENARN 

       850        860        870        880        890        900 
EKLIGGSLQA ELVLFADDSL ASKLAKLGDE LRFVLLTSKA VVKPLAEKSE AAQATDIDGL 

       910        920        930        940 
FVQVNKTEAE KCDRCWHHTP DVGTIAGHTT ICGRCVSNVE GEGEVRKFA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE003852 Genomic DNA. Translation: AAF93847.1.
PIRD82293.
RefSeqNP_230331.1. NC_002505.1.

3D structure databases

ProteinModelPortalQ9KU47.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243277.VC0682.

Protocols and materials databases

DNASU2615471.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF93847; AAF93847; VC_0682.
GeneID2615471.
KEGGvch:VC0682.
PATRIC20080481. VBIVibCho83274_0654.

Phylogenomic databases

eggNOGCOG0060.
KOK01870.
OMAVLGDWDN.
OrthoDBEOG644ZM1.
ProtClustDBPRK05743.

Enzyme and pathway databases

BioCycVCHO:VC0682-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_VIBCH
AccessionPrimary (citable) accession number: Q9KU47
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries