Beta-hexosaminidase - Vibrio cholerae

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase
    Beta-N-acetylhexosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	VC_0692

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

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Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_00364
Subunit structure	Monomer Potential.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

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Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 330

330

Beta-hexosaminidase HAMAP MF_00364

PRO_0000210800

Sites

Active site

242

1

By similarity

Secondary structure

1

.

330

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9KU37-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: A737BD82C149D3A2
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FASTA

330

36,466

        10         20         30         40         50         60 
MGPLWLDVAG YELSAEDREI LQHPTVGGVI LFGRNYHDNQ QLLALNKAIR QAAKRPILIG 

        70         80         90        100        110        120 
VDQEGGRVQR FREGFSRIPP AQYYARAENG VELAEQGGWL MAAELIAHDV DLSFAPVLDM 

       130        140        150        160        170        180 
GFACKAIGNR AFGEDVQTVL KHSSAFLRGM KAVGMATTGK HFPGHGAVIA DSHLETPYDE 

       190        200        210        220        230        240 
RETIAQDMAI FRAQIEAGVL DAMMPAHVVY PHYDAQPASG SSYWLKQVLR EELGFKGIVF 

       250        260        270        280        290        300 
SDDLSMEGAA VMGGPVERSH QALVAGCDMI LICNKREAAV EVLDNLPIME VPQAEALLKK 

       310        320        330 
QQFSYSELKR LERWQQASAN MQRLIEQFSE

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References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae."
Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S.

Fraser C.M.
Nature 406:477-483(2000) [PubMed: 10952301] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

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Cross-references

Sequence databases

AE003852 Genomic DNA. Translation: AAF93857.1.

PIR

A82292.

RefSeq

NP_230341.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TR9	X-ray	1.80	A	2-330	[»]
1Y65	X-ray	1.85	A	2-330	[»]
2OXN	X-ray	1.70	A	1-330	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH3. Glycoside Hydrolase Family 3.

Genome annotation databases

GeneID

2615481.

GenomeReviews

Gene locus VC_0692 in contig AE003852_GR.

KEGG

vch:VC0692.

TIGR

VC_0692.

Phylogenomic databases

HOGENOM

Q9KU37.

OMA

Q9KU37. FGRNIGE.

Enzyme and pathway databases

BRENDA

3.2.1.52. 19019.

Family and domain databases

HAMAP

MF_00364.
[Tree]

InterPro

IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
[Graphical view]

Gene3D

G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.

Pfam

PF00933. Glyco_hydro_3. 1 hit.
[Graphical view]

PROSITE

PS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

NAGZ_VIBCH

Accession

Primary (citable) accession number: Q9KU37

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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