Q9KU08 (PPX_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exopolyphosphatase
Short name=ExopolyPase
EC=3.6.1.11

Alternative name(s):
Metaphosphatase

Gene names

Name:	ppx
Ordered Locus Names:	VC_0722

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate By similarity.
Catalytic activity	(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the GppA/Ppx family.
Sequence caution	The sequence AAF93887.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphorus metabolic process Inferred from sequence or structural similarity Ref.2. Source: TIGR polyphosphate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	exopolyphosphatase activity Inferred from sequence or structural similarity Ref.2. Source: TIGR magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Exopolyphosphatase		PRO_0000194306
Experimental info
	Sequence conflict	456	1	T → P in AAC32884. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9KU08 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 8AD7BE0C9CB380C1	FASTA	500	56,445
	10 20 30 40 50 60 MTTVVSNARE IAAIDLGSNS FHMVVAKVVD QDLQLISRHK QRVRLAAGLD EQKNLDEESI 70 80 90 100 110 120 QRGLECLAMF AERLQGFEPR NVRIAATHTL RQARNANLFI QRALDVLPFP IEIIPGSEEA 130 140 150 160 170 180 RLIYLGVAHT QPQADSMLVV DIGGGSTEMI IGKGFEAELL NSKQMGCVNF TERYFANGKL 190 200 210 220 230 240 SRKNFAQAIV ASEQKLESIA SKYRKKGWQM AFGSSGTIKA IHEVLIGQGH EDGLITFERL 250 260 270 280 290 300 SKLIEKLCEW DSIDDLQLPG LTDDRKPVFA AGVAILSAIF HGLNIKEMHF SDGALREGLL 310 320 330 340 350 360 YEMEDRFKYS DIRLRTTENL AAKHLVDLEH AAKVKGHARE FLAQVANELG LPEGSELCDL 370 380 390 400 410 420 LEWGALLHEV GLSINLQGFH RHSAYILRHN NMAGFNSEQQ LVLSNLARFQ RKSLKLNELD 430 440 450 460 470 480 DFSLFKKKHI IGLIRVLRLA IVVNGQRNDD PLPPLTLSAK DDEWRLECEQ PDWLENNKLL 490 500 HADLQTEQEY WREVGWQLLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The polyphosphate kinase and exopolyphosphatase genes of Vibrio cholerae." Ogawa N., Fraley C., Kornberg A. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: El Tor Inaba 92A1552.
[2]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF083928 Genomic DNA. Translation: AAC32884.1. AE003852 Genomic DNA. Translation: AAF93887.1. Different initiation.
PIR	C82289.
RefSeq	NP_230371.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KU08.
SMR	Q9KU08. Positions 10-500.
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VC0722.
Protocols and materials databases
DNASU	2615731.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF93887; AAF93887; VC_0722.
GeneID	2615731.
KEGG	vch:VC0722.
PATRIC	20080563. VBIVibCho83274_0690.
Phylogenomic databases
eggNOG	COG0248.
KO	K01524.
OMA	IIGQRFE.
ProtClustDB	CLSK874085.
Family and domain databases
InterPro	IPR022371. Exopolyphosphatase. IPR003695. Ppx_GppA. [Graphical view]
Pfam	PF02541. Ppx-GppA. 1 hit. [Graphical view]
TIGRFAMs	TIGR03706. exo_poly_only. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPX_VIBCH

Accession

Primary (citable) accession number: Q9KU08
Secondary accession number(s): O86074

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	May 1, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program