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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

VC_0767

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Pathwayi: XMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes XMP from IMP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Inosine-5'-monophosphate dehydrogenase (VC_0767)
This subpathway is part of the pathway XMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from IMP, the pathway XMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei228 – 2281NAD; via amide nitrogenCombined sources
Binding sitei279 – 2791NADCombined sources
Active sitei307 – 3071Thioimidate intermediateUniRule annotation
Binding sitei442 – 4421NADCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi25 – 262NADCombined sources
Nucleotide bindingi250 – 2567NADCombined sources
Nucleotide bindingi300 – 3023NADCombined sources

GO - Molecular functioni

  • IMP dehydrogenase activity Source: TIGR
  • metal ion binding Source: UniProtKB-KW
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • GMP biosynthetic process Source: UniProtKB-KW
  • purine ribonucleotide biosynthetic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

GMP biosynthesisUniRule annotation, Purine biosynthesis

Keywords - Ligandi

Metal-bindingUniRule annotation, NADUniRule annotationCombined sources, Nucleotide-bindingCombined sources, PotassiumUniRule annotation

Enzyme and pathway databases

BioCyciVCHO:VC0767-MONOMER.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Gene namesi
Ordered Locus Names:VC_0767Imported
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)Imported
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584 Componenti: Chromosome 1

Interactioni

Protein-protein interaction databases

STRINGi243277.VC0767.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FEZX-ray2.16A/B1-92[»]
A/B200-489[»]
4FO4X-ray2.03A/B1-90[»]
A/B220-489[»]
4FXSX-ray2.24A1-489[»]
4IX2X-ray2.15A/B/C/D1-90[»]
A/B/C/D220-489[»]
4QNEX-ray2.32A/B1-90[»]
A/B220-489[»]
4QQ3X-ray1.72A1-90[»]
A220-489[»]
4X3ZX-ray1.62A/B1-90[»]
A/B220-489[»]
ProteinModelPortaliQ9KTW3.
SMRiQ9KTW3. Positions 5-487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini95 – 15460CBSInterPro annotationAdd
BLAST
Domaini155 – 21662CBSInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0516. LUCA.
COG0517. LUCA.
ENOG4105CP4. Bacteria.
KOiK00088.
OMAiSSMGYCG.
OrthoDBiEOG6GTZPV.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 2 hits.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KTW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHMLRIAKEA LTFDDVLLVP AHSTVLPNTA DLRTRLTKNI ALNIPMVSAS
60 70 80 90 100
MDTVTEARLA IALAQEGGIG FIHKNMSIEQ QAAQVHQVKI FEAGVVTHPV
110 120 130 140 150
TVRPEQTIAD VMELTHYHGF AGFPVVTENN ELVGIITGRD VRFVTDLTKS
160 170 180 190 200
VAAVMTPKER LATVKEGATG AEVQEKMHKA RVEKILVVND EFQLKGMITA
210 220 230 240 250
KDFHKAESKP NACKDEQGRL RVGAAVGAAP GNEERVKALV EAGVDVLLID
260 270 280 290 300
SSHGHSEGVL QRIRETRAAY PHLEIIGGNV ATAEGARALI EAGVSAVKVG
310 320 330 340 350
IGPGSICTTR IVTGVGVPQI TAIADAAGVA NEYGIPVIAD GGIRFSGDIS
360 370 380 390 400
KAIAAGASCV MVGSMFAGTE EAPGEVILYQ GRSYKAYRGM GSLGAMSKGS
410 420 430 440 450
SDRYFQTDNA ADKLVPEGIE GRIAYKGHLK EIIHQQMGGL RSCMGLTGSA
460 470 480
TVEDLRTKAQ FVRISGAGMK ESHVHDVQIT KEAPNYRLG
Length:489
Mass (Da):51,943
Last modified:October 1, 2000 - v1
Checksum:iB38133B52F8661C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93932.1.
PIRiC82282.
RefSeqiNP_230416.1. NC_002505.1.
WP_001880568.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93932; AAF93932; VC_0767.
GeneIDi2615310.
KEGGivch:VC0767.
PATRICi20080645. VBIVibCho83274_0731.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93932.1.
PIRiC82282.
RefSeqiNP_230416.1. NC_002505.1.
WP_001880568.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FEZX-ray2.16A/B1-92[»]
A/B200-489[»]
4FO4X-ray2.03A/B1-90[»]
A/B220-489[»]
4FXSX-ray2.24A1-489[»]
4IX2X-ray2.15A/B/C/D1-90[»]
A/B/C/D220-489[»]
4QNEX-ray2.32A/B1-90[»]
A/B220-489[»]
4QQ3X-ray1.72A1-90[»]
A220-489[»]
4X3ZX-ray1.62A/B1-90[»]
A/B220-489[»]
ProteinModelPortaliQ9KTW3.
SMRiQ9KTW3. Positions 5-487.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0767.

Protocols and materials databases

DNASUi2615310.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93932; AAF93932; VC_0767.
GeneIDi2615310.
KEGGivch:VC0767.
PATRICi20080645. VBIVibCho83274_0731.

Phylogenomic databases

eggNOGiCOG0516. LUCA.
COG0517. LUCA.
ENOG4105CP4. Bacteria.
KOiK00088.
OMAiSSMGYCG.
OrthoDBiEOG6GTZPV.

Enzyme and pathway databases

UniPathwayiUPA00601; UER00295.
BioCyciVCHO:VC0767-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 2 hits.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Inosine 5'-monophosphate dehydrogenase from Vibrio cholerae, deletion mutant."
    Osipiuk J., Maltseva N., Makowska-Grzyska M., Gu M., Anderson W.F., Joachimiak A.
    Submitted (MAY-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.16 ANGSTROMS) OF 1-92 AND 200-489.
  2. "Inosine 5'-monophosphate dehydrogenase from Vibrio cholerae, deletion mutant, complexed with IMP and mycophenolic acid."
    Osipiuk J., Maltseva N., Makowska-Grzyska M., Gu M., Anderson W.F., Joachimiak A.
    Submitted (JUN-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.03 ANGSTROMS) OF 1-90 AND 220-489.
  3. "Inosine 5'-monophosphate dehydrogenase from Vibrio cholerae complexed with IMP and mycophenolic acid."
    Osipiuk J., Maltseva N., Makowska-Grzyska M., Jedrzejczak R., Anderson W.F., Joachimiak A.
    Submitted (JUL-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.24 ANGSTROMS).
  4. "Inosine 5'-monophosphate dehydrogenase from Vibrio cholerae, deletion mutant, complexed with IMP."
    Osipiuk J., Maltseva N., Makowska-Grzyska M., Gu M., Anderson W.F., Joachimiak A.
    Submitted (JAN-2013) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 1-90 AND 220-489.
  5. "Inosine 5'-monophosphate dehydrogenase from Vibrio cholerae, deletion mutant, in complex with NAD and IMP."
    Osipiuk J., Maltseva N., Makowska-Grzyska M., Gu M., Anderson W.F., Joachimiak A.
    Submitted (JUN-2014) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.32 ANGSTROMS) OF 1-90 AND 220-489 IN COMPLEX WITH NAD.
  6. "Inosine 5'-monophosphate dehydrogenase from vibrio cholerae, deletion mutant, in complex with xmp."
    Osipiuk J., Maltseva N., Makowska-Grzyska M., Gu M., Anderson W.F., Joachimiak A.
    Submitted (JUN-2014) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.72 ANGSTROMS) OF 1-90 AND 220-489.
  7. "Inosine 5'-monophosphate dehydrogenase from Vibrio cholerae, deletion mutant, in complex with XMP and NAD."
    Center for Structural Genomics of Infectious Diseases (CSGID)
    Osipiuk J., MALTSEVA N., KIM Y., Mulligan R., MAKOWSKA-GRZYSKA M., Gu M., Anderson W.F., Joachimiak A.
    Submitted (DEC-2014) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.62 ANGSTROMS) OF 1-90 AND 220-489 IN COMPLEX WITH NAD.

Entry informationi

Entry nameiQ9KTW3_VIBCH
AccessioniPrimary (citable) accession number: Q9KTW3
Entry historyi
Integrated into UniProtKB/TrEMBL: October 1, 2000
Last sequence update: October 1, 2000
Last modified: December 9, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.