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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K+UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi: XMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes XMP from IMP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Inosine-5'-monophosphate dehydrogenase (guaB)
This subpathway is part of the pathway XMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from IMP, the pathway XMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei228NAD; via amide nitrogenCombined sources1
Binding sitei279NADCombined sources1
Metal bindingi302Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi304Potassium; via carbonyl oxygenUniRule annotation1
Binding sitei305IMPUniRule annotation1
Active sitei307Thioimidate intermediateUniRule annotation1
Metal bindingi307Potassium; via carbonyl oxygenUniRule annotation1
Active sitei403Proton acceptorUniRule annotation1
Binding sitei417IMPUniRule annotation1
Binding sitei442NADCombined sources1
Metal bindingi471Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi472Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi473Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 26NADCombined sources2
Nucleotide bindingi250 – 256NADCombined sources7
Nucleotide bindingi250 – 252NADUniRule annotation3
Nucleotide bindingi300 – 302NADUniRule annotationCombined sources3

GO - Molecular functioni

  • IMP dehydrogenase activity Source: TIGR
  • metal ion binding Source: UniProtKB-HAMAP
  • nucleotide binding Source: UniProtKB-HAMAP

GO - Biological processi

  • GMP biosynthetic process Source: UniProtKB-HAMAP
  • GTP biosynthetic process Source: GO_Central
  • purine ribonucleotide biosynthetic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

GMP biosynthesisUniRule annotation, Purine biosynthesis

Keywords - Ligandi

Metal-bindingUniRule annotation, NADUniRule annotationCombined sources, Nucleotide-bindingCombined sources, PotassiumUniRule annotation

Enzyme and pathway databases

BioCyciVCHO:VC0767-MONOMER.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenaseUniRule annotation
Short name:
IMPDUniRule annotation
Short name:
IMPDHUniRule annotation
Gene namesi
Name:guaBUniRule annotation
Ordered Locus Names:VC_0767Imported
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)Imported
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi243277.VC0767.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FEZX-ray2.16A/B1-92[»]
A/B200-489[»]
4FO4X-ray2.03A/B1-90[»]
A/B220-489[»]
4FXSX-ray2.24A1-489[»]
4IX2X-ray2.15A/B/C/D1-90[»]
A/B/C/D220-489[»]
4QNEX-ray2.32A/B1-90[»]
A/B220-489[»]
4QQ3X-ray1.72A1-90[»]
A220-489[»]
4X3ZX-ray1.62A/B1-90[»]
A/B220-489[»]
ProteinModelPortaliQ9KTW3.
SMRiQ9KTW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini95 – 154CBSInterPro annotationAdd BLAST60
Domaini155 – 216CBSInterPro annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni340 – 342IMP bindingUniRule annotation3
Regioni363 – 364IMP bindingUniRule annotation2
Regioni387 – 391IMP bindingUniRule annotation5

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation
Contains 2 CBS domains.UniRule annotation

Keywords - Domaini

CBS domainUniRule annotation, RepeatUniRule annotation

Phylogenomic databases

eggNOGiENOG4105CP4. Bacteria.
COG0516. LUCA.
COG0517. LUCA.
KOiK00088.
OMAiSSMGYCG.

Family and domain databases

CDDicd00381. IMPDH. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 2 hits.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KTW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHMLRIAKEA LTFDDVLLVP AHSTVLPNTA DLRTRLTKNI ALNIPMVSAS
60 70 80 90 100
MDTVTEARLA IALAQEGGIG FIHKNMSIEQ QAAQVHQVKI FEAGVVTHPV
110 120 130 140 150
TVRPEQTIAD VMELTHYHGF AGFPVVTENN ELVGIITGRD VRFVTDLTKS
160 170 180 190 200
VAAVMTPKER LATVKEGATG AEVQEKMHKA RVEKILVVND EFQLKGMITA
210 220 230 240 250
KDFHKAESKP NACKDEQGRL RVGAAVGAAP GNEERVKALV EAGVDVLLID
260 270 280 290 300
SSHGHSEGVL QRIRETRAAY PHLEIIGGNV ATAEGARALI EAGVSAVKVG
310 320 330 340 350
IGPGSICTTR IVTGVGVPQI TAIADAAGVA NEYGIPVIAD GGIRFSGDIS
360 370 380 390 400
KAIAAGASCV MVGSMFAGTE EAPGEVILYQ GRSYKAYRGM GSLGAMSKGS
410 420 430 440 450
SDRYFQTDNA ADKLVPEGIE GRIAYKGHLK EIIHQQMGGL RSCMGLTGSA
460 470 480
TVEDLRTKAQ FVRISGAGMK ESHVHDVQIT KEAPNYRLG
Length:489
Mass (Da):51,943
Last modified:October 1, 2000 - v1
Checksum:iB38133B52F8661C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93932.1.
PIRiC82282.
RefSeqiNP_230416.1. NC_002505.1.
WP_001880568.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93932; AAF93932; VC_0767.
GeneIDi2615310.
KEGGivch:VC0767.
PATRICi20080645. VBIVibCho83274_0731.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF93932.1.
PIRiC82282.
RefSeqiNP_230416.1. NC_002505.1.
WP_001880568.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FEZX-ray2.16A/B1-92[»]
A/B200-489[»]
4FO4X-ray2.03A/B1-90[»]
A/B220-489[»]
4FXSX-ray2.24A1-489[»]
4IX2X-ray2.15A/B/C/D1-90[»]
A/B/C/D220-489[»]
4QNEX-ray2.32A/B1-90[»]
A/B220-489[»]
4QQ3X-ray1.72A1-90[»]
A220-489[»]
4X3ZX-ray1.62A/B1-90[»]
A/B220-489[»]
ProteinModelPortaliQ9KTW3.
SMRiQ9KTW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0767.

Protocols and materials databases

DNASUi2615310.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93932; AAF93932; VC_0767.
GeneIDi2615310.
KEGGivch:VC0767.
PATRICi20080645. VBIVibCho83274_0731.

Phylogenomic databases

eggNOGiENOG4105CP4. Bacteria.
COG0516. LUCA.
COG0517. LUCA.
KOiK00088.
OMAiSSMGYCG.

Enzyme and pathway databases

UniPathwayiUPA00601; UER00295.
BioCyciVCHO:VC0767-MONOMER.

Family and domain databases

CDDicd00381. IMPDH. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 2 hits.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ9KTW3_VIBCH
AccessioniPrimary (citable) accession number: Q9KTW3
Entry historyi
Integrated into UniProtKB/TrEMBL: October 1, 2000
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.