Q9KTW2 (GUAA_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	guaA
Ordered Locus Names:	VC_0768

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_0000140203
Regions
	Domain	9 – 199	191	Glutamine amidotransferase type-1
	Domain	200 – 392	193	GMPS ATP-PPase
	Nucleotide binding	227 – 233	7	ATP By similarity
Sites
	Active site	86	1	Nucleophile By similarity
	Active site	173	1	By similarity
	Active site	175	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KTW2 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 80AC2194E1172CA5	FASTA	517	57,764
	10 20 30 40 50 60 MTKNIHDQRI LILDFGSQYT QLVARRVREI GVYCELWSWD VEEADIREFN PDGIILSGGP 70 80 90 100 110 120 ESVTEANSPR APQYVFDSGV PVFGVCYGMQ TMAEQLGGRV ATSDEREFGY AQVKISGESA 130 140 150 160 170 180 LFKDLDLTQD VWMSHGDKVV EIPADFVKIG ETDTCPYAAM ANEEKKYYGV QFHPEVTHTK 190 200 210 220 230 240 NGLQMLENFV LGVCGCERLW TSESIIEDAV ARIKEQVGND EVILGLSGGV DSSVVAMLVH 250 260 270 280 290 300 RAIGSKLTCV FVDNGLLRLN EGEQVMEMFG DKFGLNIIKV DAEERFLKAL EGIDEPEAKR 310 320 330 340 350 360 KTIGRVFVEV FDEESKKLSN AKWLAQGTIY PDVIESAASK TGKAHVIKSH HNVGGLPDDM 370 380 390 400 410 420 KMGLVEPLRE LFKDEVRKIG LELGLPYNML YRHPFPGPGL GVRVLGEVKK EYCDLLRRAD 430 440 450 460 470 480 AIFIEELHAA DLYNKVSQAF TVFLPVRSVG VMGDGRKYDW VVSLRAVETI DFMTAHWAHL 490 500 510 PYEFLGKVSN RIINEVNGIS RVVYDISGKP PATIEWE

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF93933.1.
PIR	D82282.
RefSeq	NP_230417.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q9KTW2.
SMR	Q9KTW2. Positions 3-517.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2615311.
GenomeReviews	Gene locus VC_0768 in contig AE003852_GR.
KEGG	vch:VC0768.
PATRIC	20080647. VBIVibCho83274_0732.
TIGR	VC_0768.
Phylogenomic databases
HOGENOM	HBG391775.
OMA	DGRTYEY.
PhylomeDB	Q9KTW2.
ProtClustDB	PRK00074.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_VIBCH

Accession

Primary (citable) accession number: Q9KTW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program