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Protein

Membrane-bound lytic murein transglycosylase F

Gene

mltF

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.UniRule annotation

Catalytic activityi

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei324UniRule annotation1

GO - Molecular functioni

  • catalytic activity Source: TIGR
  • exo-oligoalginate lyase activity Source: UniProtKB-EC
  • gellan lyase activity Source: UniProtKB-EC
  • lytic transglycosylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  • cell wall macromolecule catabolic process Source: UniProtKB-HAMAP
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan catabolic process Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciVCHO:VC0866-MONOMER.

Protein family/group databases

CAZyiGH23. Glycoside Hydrolase Family 23.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound lytic murein transglycosylase FUniRule annotation (EC:4.2.2.n1UniRule annotation)
Alternative name(s):
Murein lyase FUniRule annotation
Gene namesi
Name:mltFUniRule annotation
Ordered Locus Names:VC_0866
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cell outer membrane; Peripheral membrane protein

  • Note: Attached to the inner leaflet of the outer membrane.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27UniRule annotationAdd BLAST27
ChainiPRO_000035399028 – 530Membrane-bound lytic murein transglycosylase FAdd BLAST503

Interactioni

Protein-protein interaction databases

STRINGi243277.VC0866.

Structurei

3D structure databases

ProteinModelPortaliQ9KTN5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni28 – 279Non-LT domainUniRule annotationAdd BLAST252
Regioni280 – 530LT domainUniRule annotationAdd BLAST251

Domaini

The N-terminal domain does not have lytic activity and probably modulates enzymatic activity. The C-terminal domain is the catalytic active domain.UniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the bacterial solute-binding protein 3 family.UniRule annotation
In the C-terminal section; belongs to the transglycosylase Slt family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CHQ. Bacteria.
COG4623. LUCA.
KOiK18691.
OMAiKYGYARG.

Family and domain databases

HAMAPiMF_02016. MltF. 1 hit.
InterProiIPR023346. Lysozyme-like_dom.
IPR023703. MltF.
IPR001638. Solute-binding_3/MltF_N.
IPR000189. Transglyc_AS.
IPR008258. Transglycosylase_SLT_dom_1.
[Graphical view]
PANTHERiPTHR21666:SF215. PTHR21666:SF215. 1 hit.
PfamiPF00497. SBP_bac_3. 1 hit.
PF01464. SLT. 1 hit.
[Graphical view]
SMARTiSM00062. PBPb. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 2 hits.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS00922. TRANSGLYCOSYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9KTN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPFAYKLPI RALWLGLLSL LLVGCQIDSE PKSELEKIRE RGVLRVGTLN
60 70 80 90 100
NPLSYYIGPD GPTGLDYELA REFAKELGVK LEMKPAYRLS SLFPALKNGE
110 120 130 140 150
VDIIAAGLSQ SEERLKDFRA GPAYYYVSQQ VVYKKGDWRP RSFKQLVERQ
160 170 180 190 200
QTLLKDNPEL AFFSVVDDSH FEHTLLAKQQ KYPDFQFHVD SNSDVNDLLK
210 220 230 240 250
KVSQGELLFT MADSVEVSLS QRIYPELAAA FELTEDQPIS WFIRRSDDES
260 270 280 290 300
LYALMIEFFG NLKQSGYLAS LEEKYIGHIG AFDYVDTRAF IRALDTRLPR
310 320 330 340 350
WTPLFQKYSA EFDWRLVAAL AYQESHWNPY AKSPTGVRGL MMLTLPTARS
360 370 380 390 400
VGVSDRLDPE QSIRGGVEYL RRMMERVPDS ISEHEKIWFA LASYNIGYGH
410 420 430 440 450
MMDARRLTKS QGADPDSWAD VKDRLPQLQQ KKYFTQTRYG YARGDEARNY
460 470 480 490 500
VENIRRYYQS IIGHLEQRQL ATGDESIEDL TVIALDEEFF NEEANREILD
510 520 530
EEAEALESES LENSESSAEP SAKPSTESKN
Length:530
Mass (Da):60,767
Last modified:October 1, 2000 - v1
Checksum:iDAE469E9C6A332AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94028.1.
PIRiF82271.
RefSeqiNP_230513.1. NC_002505.1.
WP_000187388.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF94028; AAF94028; VC_0866.
GeneIDi2614533.
KEGGivch:VC0866.
PATRICi20080836. VBIVibCho83274_0826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA. Translation: AAF94028.1.
PIRiF82271.
RefSeqiNP_230513.1. NC_002505.1.
WP_000187388.1. NC_002505.1.

3D structure databases

ProteinModelPortaliQ9KTN5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0866.

Protein family/group databases

CAZyiGH23. Glycoside Hydrolase Family 23.

Protocols and materials databases

DNASUi2614533.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF94028; AAF94028; VC_0866.
GeneIDi2614533.
KEGGivch:VC0866.
PATRICi20080836. VBIVibCho83274_0826.

Phylogenomic databases

eggNOGiENOG4105CHQ. Bacteria.
COG4623. LUCA.
KOiK18691.
OMAiKYGYARG.

Enzyme and pathway databases

BioCyciVCHO:VC0866-MONOMER.

Family and domain databases

HAMAPiMF_02016. MltF. 1 hit.
InterProiIPR023346. Lysozyme-like_dom.
IPR023703. MltF.
IPR001638. Solute-binding_3/MltF_N.
IPR000189. Transglyc_AS.
IPR008258. Transglycosylase_SLT_dom_1.
[Graphical view]
PANTHERiPTHR21666:SF215. PTHR21666:SF215. 1 hit.
PfamiPF00497. SBP_bac_3. 1 hit.
PF01464. SLT. 1 hit.
[Graphical view]
SMARTiSM00062. PBPb. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 2 hits.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS00922. TRANSGLYCOSYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLTF_VIBCH
AccessioniPrimary (citable) accession number: Q9KTN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.