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Q9KTN2 (PUR4_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL
Ordered Locus Names:VC_0869
OrganismVibrio cholerae
Taxonomic identifier666 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length1297 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer By similarity. HAMAP MF_00419

Subcellular location

Cytoplasm By similarity HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12971297Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000100420

Regions

Domain1044 – 1297254Glutamine amidotransferase type-1
Nucleotide binding307 – 31812ATP Potential

Sites

Active site11371Nucleophile By similarity
Active site12621 By similarity
Active site12641 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KTN2 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: D24DA2875D392696

FASTA1,297141,421
        10         20         30         40         50         60 
MRILRGSPAL SEFRVNKLLT ACREQQLPVT GIYAEFMHFA DLKAELNPQE LEKLEKLLTY 

        70         80         90        100        110        120 
GPTIQEHEPQ GLLLLVTPRP GTISPWSSKA TDIAHNCGLH GIKRLERGTA YYVEAETALT 

       130        140        150        160        170        180 
AAQIATLEAL LHDRMMEVVF AELTDAQQLF SVAEPAPMSQ VDVLAGGRRA LEEANVSLGL 

       190        200        210        220        230        240 
ALAEDEIDYL VESFTKLGRN PNDIELMMFA QANSEHCRHK IFNADWTIDG VKQDKSLFKM 

       250        260        270        280        290        300 
IKNTFEQTPD YVLSAYKDNA AVMTGSTVGR FFPDPESRQY TYHHEDAHIL MKVETHNHPT 

       310        320        330        340        350        360 
AISPWPGAST GSGGEIRDEG ATGIGGKPKA GLVGFTTSNL RIPGFEQPWE SDFGKPSRIV 

       370        380        390        400        410        420 
NALDIMLEGP LGGAAFNNEF GRPNLLGYFR TYEEKVTSHA GEEVRGYHKP IMIAGGMGNI 

       430        440        450        460        470        480 
RAEHIQKKEI PVGAKLIVLG GPAMNIGLGG GAASSMASGQ SAEDLDFASV QRENPEMERR 

       490        500        510        520        530        540 
CQEVIDRCWQ LGDKNPIAFI HDVGAGGISN ALPELVNDGD RGGKFQLRNV PNDEPGMSPL 

       550        560        570        580        590        600 
EIWCNESQER YVLAVAAEDM PLFDAICQRE RAPYAVVGEA TEERHLTLED SHFANTPIDM 

       610        620        630        640        650        660 
PMDILLGKPP KMHREASTLK VSSPALERSG IELNEAVDRV LRLPAVAEKT FLITIGDRSV 

       670        680        690        700        710        720 
TGLVARDQMV GPWQVPVANC AVTAASFDSY HGEAMSMGER TPVALLDFGA SARLAVGEAI 

       730        740        750        760        770        780 
TNIAATDIGE LKRIKLSANW MSPAGHPGED AGLYEAVKAV GEELCPALGI TIPVGKDSMS 

       790        800        810        820        830        840 
MKTKWQENGE QKEVTSPLSL IITAFARVED IRKTVTPQLR TDLGETSLIL IDLGNGQNRL 

       850        860        870        880        890        900 
GATALAQVYK QLGDKPADVD NAAQLKGFFD AVQTLVRNDK LVAYHDKGDG GLLVTLAEMA 

       910        920        930        940        950        960 
FAGHCGIKAN IETLGDDALA ALFNEELGAV VQVKNDELNA VLATLAAHGL EACAHVIGEV 

       970        980        990       1000       1010       1020 
EASDRLLITC GEEVLIERSR TELRTIWAEM THKMQALRDN SACADQEFAA KQDNRDPGLN 

      1030       1040       1050       1060       1070       1080 
AKLTYDVQAD VAAPYIAKGV RPKMAILREQ GVNSHVEMAA AFDRAGFDAV DVHMSDILTG 

      1090       1100       1110       1120       1130       1140 
QTVLDAYQGL VACGGFSYGD VLGAGEGWAK SILFNAQARE QFEQFFQRKD TFSLGVCNGC 

      1150       1160       1170       1180       1190       1200 
QMLSNLRDLI PGAELWPRFV RNESDRFEAR FSLVEVQKSP SLFFSEMAGS RMPIAVSHGE 

      1210       1220       1230       1240       1250       1260 
GRVEVRDAQH LAAIEQSGTV AIRFVDNFGQ PTQAYPSNPN GSPNAITGLT TQDGRVTIMM 

      1270       1280       1290 
PHPERVFRTV ANSWHPDNWG ENGAWMRMFQ NARKYFG

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE003852 Genomic DNA. Translation: AAF94031.1.
PIRA82272.
RefSeqNP_230516.1. NC_002505.1.

3D structure databases

ProteinModelPortalQ9KTN2.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2614536.
GenomeReviewsGene locus VC_0869 in contig AE003852_GR.
KEGGvch:VC0869.
PATRIC20080842. VBIVibCho83274_0829.
TIGRVC_0869.

Phylogenomic databases

HOGENOMHBG335309.
OMAVKAVGME.
PhylomeDBQ9KTN2.
ProtClustDBPRK05297.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_VIBCH
AccessionPrimary (citable) accession number: Q9KTN2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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